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Literature summary extracted from

  • Lyu, Z.; Shao, N.; Chou, C.W.; Shi, H.; Patel, R.; Duin, E.C.; Whitman, W.B.
    Posttranslational methylation of arginine in methyl coenzyme M reductase has a profound impact on both methanogenesis and growth of Methanococcus maripaludis (2020), J. Bacteriol., 202, e00654-19 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.8.4.1 biofuel production CH4 is an important biofuel as well as a potential feedstock for the chemical industry if it can be converted by Mcr to a liquid biofuel with a high energy density Methanococcus maripaludis
2.8.4.1 environmental protection metabolization of methane can positively influence the environment Methanococcus maripaludis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.379 G140A/G144A/G146A the mutant sustains a 15.8% loss in Arg275 methylation as compared to the wild type enzyme Methanococcus maripaludis
2.1.1.379 H375F the mutant sustains a 0.5% loss in Arg275 methylation as compared to the wild type enzyme Methanococcus maripaludis
2.1.1.379 H375F/DELTAN371 the mutant sustains a 42.5% loss in Arg275 methylation as compared to the wild type enzyme Methanococcus maripaludis
2.1.1.379 H375G the mutant sustains a 0.8% loss in Arg275 methylation as compared to the wild type enzyme Methanococcus maripaludis
2.1.1.379 N212A/D213A the mutant sustains a 35% loss in Arg275 methylation as compared to the wild type enzyme Methanococcus maripaludis
2.8.4.1 additional information markerless deletion of methanogenesis marker protein 10 (Mmp10), encoded by gene mmpX, can play an important role in methanogenesis. To systematically examine its physiological role, mmpX (locus tag MMP1554), the deletion of gene encoding Mmp10 in Methanococcus maripaludis results in the complete loss of the 5-C-(S)-methylarginine PTM of residue 275 in the McrA subunit. When the DELTAmmpX mutant is complemented with the wild-type gene expressed by either a strong or a weak promoter, methylation is fully restored. Compared to the parental strain, maximal rates of methane formation by whole cells are reduced by 40 to 60% in the DELTAmmpX mutant. The reduction in activity is fully reversed by the complement with the strong promoter. Site-directed mutagenesis of mmpX result in a differential loss of arginine methylation among the mutants in vivo. Construction of diverse mutant strains from parental strain S0001, and phenotypes, overview Methanococcus maripaludis

General Stability

EC Number General Stability Organism
2.8.4.1 over 90% of the activity of resting cells harboring enzyme Mcr is lost within 1 h, Mcr from Methanococcus maripaludis is very unstable even in cell extracts Methanococcus maripaludis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.379 2 S-adenosyl-L-methionine + [methyl coenzyme-M reductase subunit McrA]-L-arginine275 + reduced acceptor Methanococcus maripaludis
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + [methyl coenzyme-M reductase subunit McrA]-(5S)-C-methyl-L-arginine275 + acceptor
-
 ?
2.8.4.1 methyl-CoM + CoB Methanococcus maripaludis
-
 CoM-S-S-CoB + methane
-
 r
2.8.4.1 methyl-CoM + CoB Methanococcus maripaludis LL
-
 CoM-S-S-CoB + methane
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.379 Methanococcus maripaludis
-
-
-
2.8.4.1 Methanococcus maripaludis Q6LWZ5 AND Q6LWZ9 AND Q6LWZ6 encoding subunit A, B, and G
-
2.8.4.1 Methanococcus maripaludis LL Q6LWZ5 AND Q6LWZ9 AND Q6LWZ6 encoding subunit A, B, and G
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.8.4.1 alkylation the posttranslational methylation of arginine in methyl coenzyme M reductase has a profound impact on both methanogenesis and growth of Methanococcus maripaludis. Protein Mmp10 is necessary for arginine methylation. Replacement of the only conserved His residue in the C-terminal half of Mmp10 with either G or F results in a loss of less than 1% of Arg275 methylation Methanococcus maripaludis
2.8.4.1 additional information the McrA subunit possesses up to five highly conserved but not universal posttranslational modifications (PTMs) at its active site, including 1-N-methyl-His261, 5-C-(S)-methyl-Arg275, 2-(S)-methyl-Gln403, and thio-Gly448, which are all found within the McrA subunit Methanococcus maripaludis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.379 2 S-adenosyl-L-methionine + [methyl coenzyme-M reductase subunit McrA]-L-arginine275 + reduced acceptor
-
 Methanococcus maripaludis S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + [methyl coenzyme-M reductase subunit McrA]-(5S)-C-methyl-L-arginine275 + acceptor
-
 ?
2.8.4.1 methyl-CoM + CoB
-
 Methanococcus maripaludis CoM-S-S-CoB + methane
-
 r
2.8.4.1 methyl-CoM + CoB
-
 Methanococcus maripaludis LL CoM-S-S-CoB + methane
-
 r

Subunits

EC Number Subunits Comment Organism
2.8.4.1 heterotrimer
-
 Methanococcus maripaludis

Synonyms

EC Number Synonyms Comment Organism
2.1.1.379 methanogenesis marker protein 10
-
 Methanococcus maripaludis
2.1.1.379 MMP10
-
 Methanococcus maripaludis
2.1.1.379 mmpX
-
 Methanococcus maripaludis
2.8.4.1 MCR
-
 Methanococcus maripaludis
2.8.4.1 mcrA subunit A Methanococcus maripaludis
2.8.4.1 mcrB subunit B Methanococcus maripaludis
2.8.4.1 mcrG subunit G Methanococcus maripaludis
2.8.4.1 MMP1555
-
 Methanococcus maripaludis
2.8.4.1 MMP1558
-
 Methanococcus maripaludis
2.8.4.1 MMP1559
-
 Methanococcus maripaludis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.4.1 37
-
 assay at Methanococcus maripaludis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.379 S-adenosyl-L-methionine dependent on Methanococcus maripaludis
2.8.4.1 F-430 coenzyme F430, during enzymatic catalysis, the Ni(I) of coenzyme F430 seen to attack the sulfur atom of methyl coenzyme M, producing a methyl radical intermediate Methanococcus maripaludis

General Information

EC Number General Information Comment Organism
2.1.1.379 metabolism the enzyme plays an important role in methanogenesis Methanococcus maripaludis
2.8.4.1 additional information the enzyme possesses two identical active sites, each of which contains up to five posttranslationally modified amino acid residues. Role of Mmp10 in Mcr methylation on Arg275 and function in methogenesis,mutational analysis, overview Methanococcus maripaludis
2.8.4.1 physiological function roles of Mcr in the global carbon cycle. CH4 is an important biofuel as well as a potential feedstock for the chemical industry if it can be converted by Mcr to a liquid biofuel with a high energy density. CH4 is also a potent greenhouse gas, increases of which are contributing to global warming Methanococcus maripaludis