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Literature summary extracted from

  • Morabbi Heravi, K.; Altenbuchner, J.
    Cross talk among transporters of the phosphoenolpyruvate-dependent phosphotransferase system in Bacillus subtilis (2018), J. Bacteriol., 200, e00213-18 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.9 expressed in Escherichia coli strain JW2409-1 Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.3.9 cytoplasm
-
 Bacillus subtilis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.9 Mg2+ 50 mM used in assay conditions Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.211 [protein]-Npi-phospho-L-histidine + sucrose[side 1] Bacillus subtilis
-
 [protein]-L-histidine + sucrose 6'-phosphate[side 2]
-
 ?
2.7.1.211 [protein]-Npi-phospho-L-histidine + sucrose[side 1] Bacillus subtilis 168
-
 [protein]-L-histidine + sucrose 6'-phosphate[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.211 Bacillus subtilis P05306
-
-
2.7.1.211 Bacillus subtilis 168 P05306
-
-
2.7.3.9 Bacillus subtilis
-
-
-
2.7.3.9 Bacillus subtilis KM0
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.9 Talon metal affinity resin column chromatography or Strep-Tactin resin column chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.211 [protein]-Npi-phospho-L-histidine + sucrose[side 1]
-
 Bacillus subtilis [protein]-L-histidine + sucrose 6'-phosphate[side 2]
-
 ?
2.7.1.211 [protein]-Npi-phospho-L-histidine + sucrose[side 1]
-
 Bacillus subtilis 168 [protein]-L-histidine + sucrose 6'-phosphate[side 2]
-
 ?
2.7.3.9 phosphoenolpyruvate + SacX
-
 Bacillus subtilis pyruvate + phosphorylated SacX
-
 ?
2.7.3.9 phosphoenolpyruvate + SacX
-
 Bacillus subtilis KM0 pyruvate + phosphorylated SacX
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.211 sacP
-
 Bacillus subtilis
2.7.1.211 sucrose-specific transporter
-
 Bacillus subtilis
2.7.3.9 phosphoenolpyruvate-dependent phosphotransferase system
-
 Bacillus subtilis
2.7.3.9 PTS
-
 Bacillus subtilis
2.7.3.9 ptsA
-
 Bacillus subtilis
2.7.3.9 YpqE
-
 Bacillus subtilis

General Information

EC Number General Information Comment Organism
2.7.3.9 metabolism enzyme expression enables cells to grow with maltose, GlcNAc, sucrose, and trehalose Bacillus subtilis