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Literature summary extracted from

  • Sheppard, C.A.; Trimmer, E.E.; Matthews, R.G.
    Purification and properties of NADH-dependent 5, 10-methylenetetrahydrofolate reductase (MetF) from Escherichia coli (1999), J. Bacteriol., 181, 718-725 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.54 expression in Escherichia coli Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.54 5,10-methylenetetrahydrofolate substrate inhibition Escherichia coli
1.5.1.54 NADH substrate inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.54 0.0008
-
 5,10-methylenetetrahydrofolate pH 7.2, 15°C Escherichia coli
1.5.1.54 0.013
-
 NADH pH 7.2, 15°C Escherichia coli
1.5.1.54 0.075
-
 5-methyltetrahydrofolate pH 7.2, 15°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.54 119000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.54 Escherichia coli P0AEZ1 cf. EC 1.5.1.20
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.54 recombinant protein Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.5.1.54 -80°C, 50 mM phosphate buffer, pH 7.2, 10% glycerol, 0.3 mM EDTA, stable for up to one year Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.54 5,10-methylenetetrahydrofolate + menadiol
-
 Escherichia coli 5-methyltetrahydrofolate + menadione
-
 r
1.5.1.54 5,10-methylenetetrahydrofolate + NADH + H+
-
 Escherichia coli 5-methyltetrahydrofolate + NAD+
-
 ?
1.5.1.54 5-methyltetrahydrofolate + menadione
-
 Escherichia coli 5,10-methylenetetrahydrofolate + menadiol
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.1.54 tetramer 4 * 33100, calculated from sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.5.1.54 metF
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.54 30
-
 NADH pH 7.2, 15°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.54 FAD 1 FAD per subunit Escherichia coli
1.5.1.54 additional information no cofactor: NADPH Escherichia coli
1.5.1.54 NADH
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.54 0.0071
-
 5,10-methylenetetrahydrofolate pH 7.2, 15°C Escherichia coli
1.5.1.54 0.009
-
 NADH pH 7.2, 15°C Escherichia coli