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Literature summary extracted from

  • Han, L.; Lobo, S.; Reynolds, K.
    Characterization of beta-ketoacyl-acyl carrier protein synthase III from Streptomyces glaucescens and its role in initiation of fatty acid biosynthesis (1998), J. Bacteriol., 180, 4481-4486 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.300 expression in Escherichia coli Streptomyces glaucescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.300 thiolactomycin 0.02 mM, about 50% inhibition Streptomyces glaucescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.300 0.0004
-
 isobutanoyl-CoA pH 7, 30°C Streptomyces glaucescens
2.3.1.300 0.0006
-
 butanoyl-CoA pH 7, 30°C Streptomyces glaucescens
2.3.1.300 0.003
-
 acetyl-CoA pH 7, 30°C Streptomyces glaucescens
2.3.1.300 0.0037
-
 malonyl-[acyl-carrier protein] pH 7, 30°C Streptomyces glaucescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.300 72000
-
 gel filtration Streptomyces glaucescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.300 (2S)-2-methylbutanoyl-CoA + malonyl-[acyl-carrier protein] Streptomyces glaucescens
-
 (4S)-4-methyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2
-
 ?
2.3.1.300 2-methylpropanoyl-CoA + malonyl-[acyl-carrier protein] Streptomyces glaucescens
-
 4-methyl-3-oxopentanoyl-[acyl-carrier-protein] + CoA + CO2
-
 ?
2.3.1.300 3-methylbutanoyl-CoA + malonyl-[acyl-carrier protein] Streptomyces glaucescens
-
 ethyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2
-
 ?
2.3.1.300 acetyl-CoA + malonyl-[acyl-carrier protein] Streptomyces glaucescens
-
 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.300 butanoyl-CoA + malonyl-[acyl-carrier protein] Streptomyces glaucescens
-
 3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2
-
 ?
2.3.1.300 isobutanoyl-CoA + malonyl-[acyl-carrier protein] Streptomyces glaucescens
-
 5-methyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2
-
 ?
2.3.1.300 additional information Streptomyces glaucescens enzyme demonstrates both beta-ketoacyl-acyl carrier protein synthase activity and acyl coenzyme A:acyl carrier protein transacylase activity in a 1:0.12 ratio ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.300 Streptomyces glaucescens Q54206 cf. EC 2.3.1.180
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.300 (2S)-2-methylbutanoyl-CoA + malonyl-[acyl-carrier protein]
-
 Streptomyces glaucescens (4S)-4-methyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2
-
 ?
2.3.1.300 2-methylpropanoyl-CoA + malonyl-[acyl-carrier protein]
-
 Streptomyces glaucescens 4-methyl-3-oxopentanoyl-[acyl-carrier-protein] + CoA + CO2
-
 ?
2.3.1.300 3-methylbutanoyl-CoA + malonyl-[acyl-carrier protein]
-
 Streptomyces glaucescens ethyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2
-
 ?
2.3.1.300 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Streptomyces glaucescens 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.300 butanoyl-CoA + malonyl-[acyl-carrier protein]
-
 Streptomyces glaucescens 3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2
-
 ?
2.3.1.300 isobutanoyl-CoA + malonyl-[acyl-carrier protein]
-
 Streptomyces glaucescens 5-methyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2
-
 ?
2.3.1.300 additional information enzyme demonstrates both beta-ketoacyl-acyl carrier protein synthase activity and acyl coenzyme A:acyl carrier protein transacylase activity in a 1:0.12 ratio Streptomyces glaucescens ?
-
-

Subunits

EC Number Subunits Comment Organism
2.3.1.300 dimer 2 * 37000, SDS-PAGE Streptomyces glaucescens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.300 3-oxoacyl-[acyl-carrier-protein] synthase
-
 Streptomyces glaucescens
2.3.1.300 FabH
-
 Streptomyces glaucescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.300 6.6 7.7
-
 Streptomyces glaucescens

General Information

EC Number General Information Comment Organism
2.3.1.300 physiological function FabH is responsible for initiating both straight- and branched-chain fatty acid biosynthesis in Streptomyces glaucescens and the ratio of the various fatty acids produced will be dictated by the ratios of the various acyl-CoA substrates that can react with FabH Streptomyces glaucescens