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Literature summary extracted from

  • Nagy, P.; Marolewski, A.; Benkovic, S.; Zalkin, H.
    Formyltetrahydrofolate hydrolase, a regulatory enzyme that functions to balance pools of tetrahydrofolate and one-carbon tetrahydrofolate adducts in Escherichia coli (1995), J. Bacteriol., 177, 1292-1298 .
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.21 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.21 ATP + formate + N1-(5-phospho-beta-D-ribosyl)glycinamide Escherichia coli
-
 ADP + phosphate + N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.21 Escherichia coli P33221
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.21 ATP + formate + N1-(5-phospho-beta-D-ribosyl)glycinamide
-
 Escherichia coli ADP + phosphate + N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.21 formate-dependent GAR transformylase
-
 Escherichia coli
6.3.1.21 PurT
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.21 ATP
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
6.3.1.21 evolution PurT is reported to have 27% amino acid sequence similarity with PurK but none with PurN Escherichia coli
6.3.1.21 malfunction on the basis of the growth of purU, purN, and purU/purN mutants, it appears that PurU provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR). Mutations in either of two pairs of genes are required to block synthesis of FGAR from GAR: purN/purT (purT encodes an alternative formate-dependent GAR transformylase) or purN/purU Escherichia coli
6.3.1.21 metabolism PurU provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) Escherichia coli
6.3.1.21 physiological function formate is required as the one-carbon donor for PurT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) and formate is produced in a PurU-dependent reaction Escherichia coli