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Literature summary extracted from

  • DeMeester, K.E.; Liang, H.; Jensen, M.R.; Jones, Z.S.; D'Ambrosio, E.A.; Scinto, S.L.; Zhou, J.; Grimes, C.L.
    Synthesis of functionalized N-acetyl muramic acids to probe bacterial cell wall recycling and biosynthesis (2018), J. Am. Chem. Soc., 140, 9458-9465 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.221 expressed in Escherichia coli strain QKU Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.99 additional information the bacterial cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM alpha-1 phosphate uridylyl transferase (MurU) are permissive to permutations at the two and three positions of the sugar donor. Utility of these derivatives in the fluorescent labeling of both Gram(-) and Gram(+) peptidoglycan in whole cells using a variety of bioorthogonal chemistries including the tetrazine ligation, allowing a rapid and scalable access to a variety of functionalized NAMs and UDP NAMs, for synthetic and purification strategies to access large quantities of these PG building blocks, and derivatives, overview Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.221 0.0303
-
2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida
2.7.1.221 0.55
-
2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida
2.7.1.221 1.12
-
2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.99 cell wall
-
Pseudomonas putida 5618
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.221 ATP + N-acetyl-D-muramate Pseudomonas putida
-
ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
?
2.7.7.99 UDP + N-acetyl-alpha-D-muramate 1-phosphate Pseudomonas putida
-
UDP-N-acetyl-alpha-D-muramate + phosphate
-
?
2.7.7.99 UDP + N-acetyl-alpha-D-muramate 1-phosphate Pseudomonas putida DSM 6125
-
UDP-N-acetyl-alpha-D-muramate + phosphate
-
?
2.7.7.99 UDP + N-acetyl-alpha-D-muramate 1-phosphate Pseudomonas putida NCIMB 11950
-
UDP-N-acetyl-alpha-D-muramate + phosphate
-
?
2.7.7.99 UDP + N-acetyl-alpha-D-muramate 1-phosphate Pseudomonas putida ATCC 47054
-
UDP-N-acetyl-alpha-D-muramate + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.221 Pseudomonas putida
-
-
-
2.7.7.99 Pseudomonas putida Q88QT2
-
-
2.7.7.99 Pseudomonas putida ATCC 47054 Q88QT2
-
-
2.7.7.99 Pseudomonas putida DSM 6125 Q88QT2
-
-
2.7.7.99 Pseudomonas putida NCIMB 11950 Q88QT2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.221 ATP + 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
Pseudomonas putida ADP + 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
?
2.7.1.221 ATP + 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
Pseudomonas putida ADP + 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
?
2.7.1.221 ATP + 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
Pseudomonas putida ADP + 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
?
2.7.1.221 ATP + N-acetyl-D-muramate
-
Pseudomonas putida ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
?
2.7.7.99 UDP + N-acetyl-alpha-D-muramate 1-phosphate
-
Pseudomonas putida UDP-N-acetyl-alpha-D-muramate + phosphate
-
?
2.7.7.99 UDP + N-acetyl-alpha-D-muramate 1-phosphate
-
Pseudomonas putida DSM 6125 UDP-N-acetyl-alpha-D-muramate + phosphate
-
?
2.7.7.99 UDP + N-acetyl-alpha-D-muramate 1-phosphate
-
Pseudomonas putida NCIMB 11950 UDP-N-acetyl-alpha-D-muramate + phosphate
-
?
2.7.7.99 UDP + N-acetyl-alpha-D-muramate 1-phosphate
-
Pseudomonas putida ATCC 47054 UDP-N-acetyl-alpha-D-muramate + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.221 amgK
-
Pseudomonas putida
2.7.1.221 MurNAc/GlcNAc anomeric kinase
-
Pseudomonas putida
2.7.7.99 murU
-
Pseudomonas putida
2.7.7.99 NAM alpha-1 phosphate uridylyl transferase
-
Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.221 4.67
-
2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida
2.7.1.221 9.74
-
2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida
2.7.1.221 14.07
-
2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida

General Information

EC Number General Information Comment Organism
2.7.7.99 metabolism the enzyme is involved in peptidoglycan cell wall biosynthesis Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.221 12.6
-
2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida
2.7.1.221 18
-
2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida
2.7.1.221 154
-
2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida