EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.221 | expressed in Escherichia coli strain QKU | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.99 | additional information | the bacterial cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM alpha-1 phosphate uridylyl transferase (MurU) are permissive to permutations at the two and three positions of the sugar donor. Utility of these derivatives in the fluorescent labeling of both Gram(-) and Gram(+) peptidoglycan in whole cells using a variety of bioorthogonal chemistries including the tetrazine ligation, allowing a rapid and scalable access to a variety of functionalized NAMs and UDP NAMs, for synthetic and purification strategies to access large quantities of these PG building blocks, and derivatives, overview | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.221 | 0.0303 | - |
2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose | pH and temperature not specified in the publication | Pseudomonas putida | |
2.7.1.221 | 0.55 | - |
2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose | pH and temperature not specified in the publication | Pseudomonas putida | |
2.7.1.221 | 1.12 | - |
2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose | pH and temperature not specified in the publication | Pseudomonas putida |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.7.99 | cell wall | - |
Pseudomonas putida | 5618 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.221 | ATP + N-acetyl-D-muramate | Pseudomonas putida | - |
ADP + N-acetyl-alpha-D-muramate 1-phosphate | - |
? | |
2.7.7.99 | UDP + N-acetyl-alpha-D-muramate 1-phosphate | Pseudomonas putida | - |
UDP-N-acetyl-alpha-D-muramate + phosphate | - |
? | |
2.7.7.99 | UDP + N-acetyl-alpha-D-muramate 1-phosphate | Pseudomonas putida DSM 6125 | - |
UDP-N-acetyl-alpha-D-muramate + phosphate | - |
? | |
2.7.7.99 | UDP + N-acetyl-alpha-D-muramate 1-phosphate | Pseudomonas putida NCIMB 11950 | - |
UDP-N-acetyl-alpha-D-muramate + phosphate | - |
? | |
2.7.7.99 | UDP + N-acetyl-alpha-D-muramate 1-phosphate | Pseudomonas putida ATCC 47054 | - |
UDP-N-acetyl-alpha-D-muramate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.221 | Pseudomonas putida | - |
- |
- |
2.7.7.99 | Pseudomonas putida | Q88QT2 | - |
- |
2.7.7.99 | Pseudomonas putida ATCC 47054 | Q88QT2 | - |
- |
2.7.7.99 | Pseudomonas putida DSM 6125 | Q88QT2 | - |
- |
2.7.7.99 | Pseudomonas putida NCIMB 11950 | Q88QT2 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.221 | ATP + 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose | - |
Pseudomonas putida | ADP + 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose | - |
? | |
2.7.1.221 | ATP + 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose | - |
Pseudomonas putida | ADP + 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose | - |
? | |
2.7.1.221 | ATP + 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose | - |
Pseudomonas putida | ADP + 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose | - |
? | |
2.7.1.221 | ATP + N-acetyl-D-muramate | - |
Pseudomonas putida | ADP + N-acetyl-alpha-D-muramate 1-phosphate | - |
? | |
2.7.7.99 | UDP + N-acetyl-alpha-D-muramate 1-phosphate | - |
Pseudomonas putida | UDP-N-acetyl-alpha-D-muramate + phosphate | - |
? | |
2.7.7.99 | UDP + N-acetyl-alpha-D-muramate 1-phosphate | - |
Pseudomonas putida DSM 6125 | UDP-N-acetyl-alpha-D-muramate + phosphate | - |
? | |
2.7.7.99 | UDP + N-acetyl-alpha-D-muramate 1-phosphate | - |
Pseudomonas putida NCIMB 11950 | UDP-N-acetyl-alpha-D-muramate + phosphate | - |
? | |
2.7.7.99 | UDP + N-acetyl-alpha-D-muramate 1-phosphate | - |
Pseudomonas putida ATCC 47054 | UDP-N-acetyl-alpha-D-muramate + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.221 | amgK | - |
Pseudomonas putida |
2.7.1.221 | MurNAc/GlcNAc anomeric kinase | - |
Pseudomonas putida |
2.7.7.99 | murU | - |
Pseudomonas putida |
2.7.7.99 | NAM alpha-1 phosphate uridylyl transferase | - |
Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.221 | 4.67 | - |
2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose | pH and temperature not specified in the publication | Pseudomonas putida | |
2.7.1.221 | 9.74 | - |
2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose | pH and temperature not specified in the publication | Pseudomonas putida | |
2.7.1.221 | 14.07 | - |
2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose | pH and temperature not specified in the publication | Pseudomonas putida |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.7.99 | metabolism | the enzyme is involved in peptidoglycan cell wall biosynthesis | Pseudomonas putida |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.221 | 12.6 | - |
2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose | pH and temperature not specified in the publication | Pseudomonas putida | |
2.7.1.221 | 18 | - |
2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose | pH and temperature not specified in the publication | Pseudomonas putida | |
2.7.1.221 | 154 | - |
2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose | pH and temperature not specified in the publication | Pseudomonas putida |