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Literature summary extracted from

  • Song, K.; Li, Y.; He, H.; Liu, L.; Zhao, P.; Xia, Q.; Wang, Y.
    A novel adenosine kinase from Bombyx mori enzymatic activity, structure, and biological function (2019), Int. J. Mol. Sci., 20, 3732 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.20
-
 Bombyx mori

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.20 E229A complete loss of activity, decrease in the alpha-helix content Bombyx mori
2.7.1.20 E229R complete loss of activity, significant decrease in the alpha-helix content Bombyx mori
2.7.1.20 F303A complete loss of activity, decrease in the alpha-helix content Bombyx mori
2.7.1.20 G280A mutation has no significant influence on the enzymatic activity compared with that of wild-type enzyme, slight decrease in the alpha-helix content Bombyx mori
2.7.1.20 G280D mutation has no significant influence on the enzymatic activity compared with that of wild-type enzyme, slight decrease in the alpha-helix content Bombyx mori
2.7.1.20 G68A mutation results in significant decrease in activity Bombyx mori
2.7.1.20 S201A mutation significantly increases the alpha-helix content of BmADK and its activity Bombyx mori
2.7.1.20 S201D significant decrease in the alpha-helix content Bombyx mori
2.7.1.20 S201R significant decrease in the alpha-helix content Bombyx mori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.20 cytoplasm
-
 Bombyx mori 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.20 38000
-
 gel filtration Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.20 ATP + adenosine Bombyx mori adenosine kinase (ADK) is the first enzyme in the adenosine remediation pathway that catalyzes adenosine phosphorylation into adenosine monophosphate, thus regulating adenosine homeostasis in cells ADP + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Bombyx mori Q2F635
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20
-
 Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.20 silk gland highly expressed in the silk gland during the pre-pupal stage Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + adenosine
-
 Bombyx mori ADP + AMP
-
 ?
2.7.1.20 ATP + adenosine adenosine kinase (ADK) is the first enzyme in the adenosine remediation pathway that catalyzes adenosine phosphorylation into adenosine monophosphate, thus regulating adenosine homeostasis in cells Bombyx mori ADP + AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.20 monomer 1 * 38000, SDS-PAGE Bombyx mori

Synonyms

EC Number Synonyms Comment Organism
2.7.1.20 ADK
-
 Bombyx mori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.20 30
-
 stable below Bombyx mori
2.7.1.20 46.51
-
 melting temperature Bombyx mori

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.20 5 11 stable Bombyx mori

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.20 Bombyx mori calculated from sequence
-
 5.13

General Information

EC Number General Information Comment Organism
2.7.1.20 metabolism adenosine kinase (ADK) is the first enzyme in the adenosine remediation pathway that catalyzes adenosine phosphorylation into adenosine monophosphate, thus regulating adenosine homeostasis in cells. RNA interference reveals the downregulation of the enzyme (BmADK) decreases ATG-8, caspase-9, Ec-R, E74A, and Br-C expression, indicating it is likely involved in 20E signaling, apoptosis, and autophagy to regulate silk gland degeneration and silkworm metamorphosis Bombyx mori