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Literature summary extracted from

  • Jelsbak, L.; Mortensen, M.; Kilstrup, M.; Olsen, J.
    The in vitro redundant enzymes PurN and PurT are both essential for systemic infection of mice in Salmonella enterica serovar Typhimurium (2016), Infect. Immun., 84, 2076-2085 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
6.3.1.21 additional information single mutants of Salmonella enterica serovar Typhimurium strain 4/74 are created by deletion of the purN and purT genes, generation of different purT (encoding formyltetrahydrofolate deformylase, EC 3.5.1.10) and purU mutants. The DELTApurU/DELTApurT mutant shows increased virulence in macophages compared to wild-type, while all other mutants have reduced virulence. purN and purT mutants grow somewhat slower than the wild-type. Mutation of purN but not purT attenuates the strain during interaction with cultured macrophages. Single-gene deletions of each of the purT and purN causes in mouse infections. While the DELTApurT mutant multiplies as fast as the wild-type strain in cultured J774A.1 macrophages, net multiplication of the DELTApurN mutant is reduced approximately 50% in 20 h. The attenuation of the DELTApurT mutant is abolished by simultaneous removal of the enzyme PurU, responsible for the formation of formate, indicating that the attenuation is related to formate accumulation or wasteful consumption of formyl tetrahydrofolate by PurU Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.21 Mg2+ required Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.21 ATP + formate + N1-(5-phospho-beta-D-ribosyl)glycinamide Salmonella enterica subsp. enterica serovar Typhimurium
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 ADP + phosphate + N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide
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 ?
6.3.1.21 ATP + formate + N1-(5-phospho-beta-D-ribosyl)glycinamide Salmonella enterica subsp. enterica serovar Typhimurium 4/74
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 ADP + phosphate + N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide
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 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.21 Salmonella enterica subsp. enterica serovar Typhimurium E8X9F0
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6.3.1.21 Salmonella enterica subsp. enterica serovar Typhimurium 4/74 E8X9F0
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.21 ATP + formate + N1-(5-phospho-beta-D-ribosyl)glycinamide
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 Salmonella enterica subsp. enterica serovar Typhimurium ADP + phosphate + N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide
-
 ?
6.3.1.21 ATP + formate + N1-(5-phospho-beta-D-ribosyl)glycinamide
-
 Salmonella enterica subsp. enterica serovar Typhimurium 4/74 ADP + phosphate + N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.21 PurT
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 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.21 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium

General Information

EC Number General Information Comment Organism
6.3.1.21 malfunction single mutants of Salmonella enterica serovar Typhimurium strain 4/74, created by deletion of the purN and purT genes, grow as well as the parental wild-type strain in minimal medium, while the double mutant does not grow. Mutation of purN but not purT attenuates the strain during interaction with cultured macrophages. Growth phenotypes of mutants in murine J774A.1 macrophages, overview Salmonella enterica subsp. enterica serovar Typhimurium
6.3.1.21 metabolism formate is produced from fTHF by the enzyme PurU Salmonella enterica subsp. enterica serovar Typhimurium
6.3.1.21 physiological function the enzymes PurN and PurT are both essential for systemic infection of mice in Salmonella enterica serovar Typhimurium. PurN and PurT are redundant in vitro but not in vivo Salmonella enterica subsp. enterica serovar Typhimurium