EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.69 | quantitative RT-PCR enzyme expression analysis | Oryza sativa Japonica Group |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.69 | additional information | construction of hemizygous and homozygous OsGGP overexpressing plants using the constitutive dual CaMV 35S promoter and Agrobacterium-mediated transformation leading to 5-9fold increased ascorbate concentrations in homozygous plants compared to low amounts in heterozygous and wild-type plants. Phenotype analysis, overview. Ascorbate concentrations are positively correlated with ferritin cn Caco-2 cells exposed to in vitro digests of NS and 35S-OsGGP BR and GBR samples | Oryza sativa Japonica Group |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.69 | GDP-beta-L-galactose + alpha-D-mannose 1-phosphate | Oryza sativa Japonica Group | - |
beta-L-galactose 1-phosphate + GDP-alpha-D-mannose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.69 | Oryza sativa Japonica Group | Q2QWM9 | cv. Nipponbare | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.7.69 | leaf | - |
Oryza sativa Japonica Group | - |
2.7.7.69 | seedling | - |
Oryza sativa Japonica Group | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.69 | GDP-beta-L-galactose + alpha-D-mannose 1-phosphate | - |
Oryza sativa Japonica Group | beta-L-galactose 1-phosphate + GDP-alpha-D-mannose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.69 | OsGGP | - |
Oryza sativa Japonica Group |
2.7.7.69 | Vtc2 | - |
Oryza sativa Japonica Group |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.7.69 | malfunction | ascorbate concentrations are negligible in both null segregant (NS) and 35S-OsGGP brown rice (BR, unpolished grain), but significantly increased in 35S-OsGGP germinated brown rice (GBR) relative to NS. Foliar ascorbate concentrations are significantly increased in 35S-OsGGP plants in the vegetative growth phase relative to NS, but significantly reduced at the reproductive growth phase and are associated with reduced OsGGP transcript levels. The 35S-OsGGP plants do not display altered salt tolerance at the vegetative growth phase despite having elevated ascorbate concentrations | Oryza sativa Japonica Group |
2.7.7.69 | metabolism | GDP-L-galactose phosphorylase is the rate-limiting enzyme of the L-galactose pathway | Oryza sativa Japonica Group |