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Literature summary extracted from

  • Weinsanto, I.; Mouheiche, J.; Laux-Biehlmann, A.; Delalande, F.; Marquette, A.; Chavant, V.; Gabel, F.; Cianferani, S.; Charlet, A.; Parat, M.O.; Goumon, Y.
    Morphine binds creatine kinase B and inhibits its activity (2018), Front. Cell. Neurosci., 12, 464 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.2 expressed in Escherichia coli BL21(DE3) cells Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.3.2 morphine 0.00001-0.1 mM morphine significantly reduces recombinant enzymatic activity (27% inhibition at 0.001 mM, 80% inhibition at more than 0.05 mM); 27% inhibition at 0.001 m, 80% inhibition at more than 0.05 mM Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.2 84000
-
 gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.3.2 ADP + phosphocreatine Mus musculus
-
 ATP + creatine
-
 r
2.7.3.2 ATP + creatine Mus musculus
-
 ADP + phosphocreatine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Mus musculus Q04447
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.2 Ni-Hitrap column chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 astrocyte
-
 Mus musculus
-
2.7.3.2 brain
-
 Mus musculus
-
2.7.3.2 neuron
-
 Mus musculus
-
2.7.3.2 oligodendrocyte
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ADP + phosphocreatine
-
 Mus musculus ATP + creatine
-
 r
2.7.3.2 ATP + creatine
-
 Mus musculus ADP + phosphocreatine
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.3.2 homodimer 2 * 42000, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.7.3.2 CK-B
-
 Mus musculus
2.7.3.2 creatine kinase B
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.3.2 ATP
-
 Mus musculus