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Literature summary extracted from

  • Singh, A.; Zhang, Y.; Zhu, K.; Subramanian, C.; Li, Z.; Jayaswal, R.; Gatto, C.; Rock, C.; Wilkinson, B.
    FabH selectivity for anteiso branched-chain fatty acid precursors in low-temperature adaptation in Listeria monocytogenes (2009), FEMS Microbiol. Lett., 301, 188-192 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.300 expression in Escherichia coli Listeria monocytogenes serotype 1/2a

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.300 0.0053
-
 isovaleryl-CoA pH 7, 30°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.0063
-
 (2S)-2-methylbutanoyl-CoA pH 7, 10°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.011
-
 (2S)-2-methylbutanoyl-CoA pH 7, 30°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.0169
-
 isovaleryl-CoA pH 7, 10°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.0243
-
 isobutanoyl-CoA pH 7, 30°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.0307
-
 isobutanoyl-CoA pH 7, 10°C Listeria monocytogenes serotype 1/2a

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.300 Listeria monocytogenes serotype 1/2a C0LNR0 cf. EC 2.3.1.180
-
2.3.1.300 Listeria monocytogenes serotype 1/2a 10403S C0LNR0 cf. EC 2.3.1.180
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.300 (2S)-2-methylbutanoyl-CoA + malonyl-[acyl-carrier protein]
-
 Listeria monocytogenes serotype 1/2a (4S)-4-methyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2
-
 ?
2.3.1.300 (2S)-2-methylbutanoyl-CoA + malonyl-[acyl-carrier protein]
-
 Listeria monocytogenes serotype 1/2a 10403S (4S)-4-methyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2
-
 ?
2.3.1.300 isobutanoyl-CoA + malonyl-[acyl-carrier protein]
-
 Listeria monocytogenes serotype 1/2a ? + CoA + CO2
-
 ?
2.3.1.300 isobutanoyl-CoA + malonyl-[acyl-carrier protein]
-
 Listeria monocytogenes serotype 1/2a 10403S ? + CoA + CO2
-
 ?
2.3.1.300 isovaleryl-CoA + malonyl-[acyl-carrier protein]
-
 Listeria monocytogenes serotype 1/2a ? + CoA + CO2
-
 ?
2.3.1.300 isovaleryl-CoA + malonyl-[acyl-carrier protein]
-
 Listeria monocytogenes serotype 1/2a 10403S ? + CoA + CO2
-
 ?
2.3.1.300 additional information the order of catalytic efficiency (in decreasing order) is: 2-methylbutyryl-CoA, isovaleryl-CoA, isobutyryl-CoA, acetyl-CoA Listeria monocytogenes serotype 1/2a ?
-
-
2.3.1.300 additional information the order of catalytic efficiency (in decreasing order) is: 2-methylbutyryl-CoA, isovaleryl-CoA, isobutyryl-CoA, acetyl-CoA Listeria monocytogenes serotype 1/2a 10403S ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.300 LMRG_01630
-
 Listeria monocytogenes serotype 1/2a

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.300 0.018
-
 isovaleryl-CoA pH 7, 10°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.047
-
 (2S)-2-methylbutanoyl-CoA pH 7, 10°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.102
-
 isobutanoyl-CoA pH 7, 10°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.12
-
 isovaleryl-CoA pH 7, 30°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.32
-
 isobutanoyl-CoA pH 7, 30°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.46
-
 (2S)-2-methylbutanoyl-CoA pH 7, 30°C Listeria monocytogenes serotype 1/2a

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.300 0.003
-
 isobutanoyl-CoA pH 7, 10°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.007
-
 (2S)-2-methylbutanoyl-CoA pH 7, 10°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.013
-
 isobutanoyl-CoA pH 7, 30°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.017
-
 isovaleryl-CoA pH 7, 10°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.023
-
 isovaleryl-CoA pH 7, 30°C Listeria monocytogenes serotype 1/2a
2.3.1.300 0.042
-
 (2S)-2-methylbutanoyl-CoA pH 7, 30°C Listeria monocytogenes serotype 1/2a