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Literature summary extracted from

  • Kuivanen, J.; Richard, P.
    NADPH-dependent 5-keto-D-gluconate reductase is a part of the fungal pathway for D-glucuronate catabolism (2018), FEBS Lett., 592, 71-77 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.69 3.2
-
 5-Dehydro-D-gluconate pH 7.0, temperature not specified in the publication Aspergillus niger
1.1.1.69 3.2
-
 5-Dehydro-D-gluconate pH 7, temperature not specified in the publication Aspergillus niger
1.1.1.69 8.4
-
 D-gluconate pH 8.0, temperature not specified in the publication Aspergillus niger
1.1.1.69 8.4
-
 D-gluconate pH 8, temperature not specified in the publication Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.69 Aspergillus niger G3XRE5
-
-
1.1.1.69 Aspergillus niger ATCC 1015 G3XRE5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.69
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.69 5-dehydro-D-gluconate + NADPH + H+
-
 Aspergillus niger D-gluconate + NADP+
-
 r
1.1.1.69 5-dehydro-D-gluconate + NADPH + H+
-
 Aspergillus niger ATCC 1015 D-gluconate + NADP+
-
 r
1.1.1.69 D-gluconate + NADP+
-
 Aspergillus niger 5-dehydro-D-gluconate + NADPH + H+
-
 r
1.1.1.69 D-gluconate + NADP+
-
 Aspergillus niger ATCC 1015 5-dehydro-D-gluconate + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.69 5-keto-D-gluconate reductase
-
 Aspergillus niger
1.1.1.69 GluF
-
 Aspergillus niger
1.1.1.69 NADPH-dependent 5-keto-D-gluconate reductase
-
 Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.69 2 3.7 5-Dehydro-D-gluconate pH 7.0, temperature not specified in the publication Aspergillus niger
1.1.1.69 2 3.7 5-Dehydro-D-gluconate pH 7, temperature not specified in the publication Aspergillus niger
1.1.1.69 2.6
-
 D-gluconate pH 8.0, temperature not specified in the publication Aspergillus niger
1.1.1.69 2.6
-
 D-gluconate pH 8, temperature not specified in the publication Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.69 additional information no activity with NAD+/NADH Aspergillus niger
1.1.1.69 NADP+
-
 Aspergillus niger
1.1.1.69 NADPH
-
 Aspergillus niger

General Information

EC Number General Information Comment Organism
1.1.1.69 malfunction the disruption of the gene, gluF, by CRISPR/Cas9 in the filamentous fungus Aspergillus niger results in a strain unable to catabolise D-glucuronate Aspergillus niger
1.1.1.69 metabolism the enzyme is involved in the pathway for D-glucuronate catabolism in fungi Aspergillus niger
1.1.1.69 physiological function gene disruption results in a strain unable to catabolise D-glucuronate. A pathway for D-glucuronate catabolism is suggested with the intermediates L-gulonate, 2-keto-L-gulonate, L-idonate, 5-keto-D-gluconate, D-gluconate and D-gluconate-6-phosphate Aspergillus niger