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Literature summary extracted from

  • Nguyen, S.; Jovcevski, B.; Pukala, T.L.; Bruning, J.B.
    Nucleoside selectivity of Aspergillus fumigatus nucleoside-diphosphate kinase (2020), FEBS J., 288, 2398-2417 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.6 hanging drop vapour diffusion Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.6 0.402
-
 GTP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 0.513
-
 ATP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 0.617
-
 ITP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 1.32
-
 CTP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 1.35
-
 UTP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 2.23
-
 TTP pH 8.0, 30°C Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Aspergillus fumigatus Q7Z8P9
-
-
2.7.4.6 Aspergillus fumigatus ATCC MYA-4609 Q7Z8P9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6
-
 Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ADP + ATP
-
 Aspergillus fumigatus ATP + ADP
-
 ?
2.7.4.6 ADP + ATP
-
 Aspergillus fumigatus ATCC MYA-4609 ATP + ADP
-
 ?
2.7.4.6 ADP + CTP
-
 Aspergillus fumigatus ATP + CDP
-
 ?
2.7.4.6 ADP + CTP
-
 Aspergillus fumigatus ATCC MYA-4609 ATP + CDP
-
 ?
2.7.4.6 ADP + GTP
-
 Aspergillus fumigatus ATP + GDP
-
 ?
2.7.4.6 ADP + GTP
-
 Aspergillus fumigatus ATCC MYA-4609 ATP + GDP
-
 ?
2.7.4.6 ADP + ITP
-
 Aspergillus fumigatus ATP + IDP
-
 ?
2.7.4.6 ADP + TTP
-
 Aspergillus fumigatus ATP + TDP
-
 ?
2.7.4.6 ADP + UTP
-
 Aspergillus fumigatus ATP + UDP
-
 ?
2.7.4.6 ADP + UTP
-
 Aspergillus fumigatus ATCC MYA-4609 ATP + UDP
-
 ?
2.7.4.6 additional information adenine nucleosides are used preferentially compared to cytidine nucleosides Aspergillus fumigatus ?
-
-
2.7.4.6 additional information adenine nucleosides are used preferentially compared to cytidine nucleosides Aspergillus fumigatus ATCC MYA-4609 ?
-
-

Subunits

EC Number Subunits Comment Organism
2.7.4.6 hexamer based on crystallographic symmetry, the enzyme forms a hexamer which is arranged as a trimer of dimers Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 Afu5g03490
-
 Aspergillus fumigatus
2.7.4.6 NDK
-
 Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.6 53.1
-
 CTP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 83.2
-
 ITP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 140
-
 UTP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 178
-
 TTP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 188
-
 GTP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 321
-
 ATP pH 8.0, 30°C Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
2.7.4.6 drug target as an essential enzyme in Aspergillus fumigatus, nucleoside-diphosphate kinase is an attractive target for antifungals Aspergillus fumigatus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.4.6 40.2
-
 CTP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 79.8
-
 TTP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 104
-
 UTP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 135
-
 ITP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 468
-
 GTP pH 8.0, 30°C Aspergillus fumigatus
2.7.4.6 626
-
 ATP pH 8.0, 30°C Aspergillus fumigatus