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Literature summary extracted from

  • Sarmiento-Pavia, P.D.; Rodriguez-Hernandez, A.; Rodriguez-Romero, A.; Sosa-Torres, M.E.
    The structure of a novel membrane-associated 6-phosphogluconate dehydrogenase from Gluconacetobacter diazotrophicus (Gd6PGD) reveals a subfamily of short-chain 6PGDs (2021), FEBS J., 288, 1286-1304 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.343 hanging-drop vapor diffusion method at 18°C Gluconacetobacter diazotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.343 0.0334
-
 6-phospho-D-gluconate pH 7.5, temperature not specified in the publication, membrane-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 0.0529
-
 NAD+ pH 7.5, temperature not specified in the publication, membrane-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 0.103
-
 NAD+ pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 0.869
-
 NADP+ pH 7.5, temperature not specified in the publication, membrane-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 0.934
-
 6-phospho-D-gluconate pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 2.393
-
 NADP+ pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme Gluconacetobacter diazotrophicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.343 cytoplasm membrane-associated. The enzyme is located in both the cytoplasm and membrane Gluconacetobacter diazotrophicus 5737
-
1.1.1.343 membrane membrane-associated. The enzyme is located in both the cytoplasm and membrane Gluconacetobacter diazotrophicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.343 70000
-
 non-denaturing PAGE Gluconacetobacter diazotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.343 6-phospho-D-gluconate + NAD+ Gluconacetobacter diazotrophicus the enzyme catalyzes an important reaction in the oxidative pentose phosphate pathway, producing a ribose precursor essential for nucleotide and nucleic acid synthesis D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.343 6-phospho-D-gluconate + NAD+ Gluconacetobacter diazotrophicus ATCC 49037 the enzyme catalyzes an important reaction in the oxidative pentose phosphate pathway, producing a ribose precursor essential for nucleotide and nucleic acid synthesis D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.343 Gluconacetobacter diazotrophicus A9H324
-
-
1.1.1.343 Gluconacetobacter diazotrophicus ATCC 49037 A9H324
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.343
-
 Gluconacetobacter diazotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.343 6-phospho-D-gluconate + NAD+ the enzyme catalyzes an important reaction in the oxidative pentose phosphate pathway, producing a ribose precursor essential for nucleotide and nucleic acid synthesis Gluconacetobacter diazotrophicus D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.343 6-phospho-D-gluconate + NAD+ the enzyme from the soluble fraction prefers NAD+ 8.4 times over NADP+ Gluconacetobacter diazotrophicus D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.343 6-phospho-D-gluconate + NAD+ the enzyme catalyzes an important reaction in the oxidative pentose phosphate pathway, producing a ribose precursor essential for nucleotide and nucleic acid synthesis Gluconacetobacter diazotrophicus ATCC 49037 D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.343 6-phospho-D-gluconate + NAD+ the enzyme from the soluble fraction prefers NAD+ 8.4 times over NADP+ Gluconacetobacter diazotrophicus ATCC 49037 D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.343 6-phospho-D-gluconate + NADP+ the enzyme from the soluble fraction prefers NAD+ 8.4 times over NADP+ Gluconacetobacter diazotrophicus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
1.1.1.343 6-phospho-D-gluconate + NADP+ the enzyme from the soluble fraction prefers NAD+ 8.4 times over NADP+ Gluconacetobacter diazotrophicus ATCC 49037 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.343 homodimer 2 * 35000, SDS-PAGE Gluconacetobacter diazotrophicus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.343 6-phosphogluconate dehydrogenase
-
 Gluconacetobacter diazotrophicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.343 0.97
-
 6-phospho-D-gluconate pH 7.5, temperature not specified in the publication, membrane-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 1.74
-
 NADP+ pH 7.5, temperature not specified in the publication, membrane-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 2.62
-
 NAD+ pH 7.5, temperature not specified in the publication, membrane-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 2.8
-
 NADP+ pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 3.63
-
 6-phospho-D-gluconate pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 6.06
-
 NAD+ pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme Gluconacetobacter diazotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.343 NAD+ the enzyme from the soluble fraction prefers NAD+ 8.4 times over NADP+ Gluconacetobacter diazotrophicus
1.1.1.343 NADP+ the enzyme from the soluble fraction prefers NAD+ 8.4 times over NADP+ Gluconacetobacter diazotrophicus

General Information

EC Number General Information Comment Organism
1.1.1.343 physiological function the enzyme catalyzes an important reaction in the oxidative pentose phosphate pathway, producing a ribose precursor essential for nucleotide and nucleic acid synthesis Gluconacetobacter diazotrophicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.343 1.2
-
 NADP+ pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 2
-
 NADP+ pH 7.5, temperature not specified in the publication, membrane-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 3.9
-
 6-phospho-D-gluconate pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 29
-
 6-phospho-D-gluconate pH 7.5, temperature not specified in the publication, membrane-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 49
-
 NAD+ pH 7.5, temperature not specified in the publication, membrane-purified enzyme Gluconacetobacter diazotrophicus
1.1.1.343 59
-
 NAD+ pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme Gluconacetobacter diazotrophicus