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Literature summary extracted from

  • Sienkiewicz, N.; Ong, H.B.; Fairlamb, A.H.
    Characterisation of a putative glutamate 5-kinase from Leishmania donovani (2018), FEBS J., 285, 2662-2678 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.11 expressed in Escherichia coli C41(DE3) pLysS cells Leishmania donovani

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.11 3,4-dehydro-L-proline 9% residual activity at 1 mM Leishmania donovani
2.7.2.11 alpha-methyl-L-proline 82% residual activity at 1 mM Leishmania donovani
2.7.2.11 cis-4-hydroxy-L-proline 87% residual activity at 1 mM Leishmania donovani
2.7.2.11 L-4-thioproline 66% residual activity at 1 mM Leishmania donovani
2.7.2.11 L-azetidine-2-carboxylic acid 25% residual activity at 1 mM Leishmania donovani
2.7.2.11 L-prolinamide 96% residual activity at 1 mM Leishmania donovani
2.7.2.11 L-proline 4% residual activity at 1 mM Leishmania donovani
2.7.2.11 L-trans-pyrrolidine-2,4-dicarboxylic acid 84% residual activity at 1 mM Leishmania donovani
2.7.2.11 Mg2+
-
 Leishmania donovani
2.7.2.11 additional information L-pipecolinic acid, D-pipecolinic acid, N-methyl-L-proline, cis-3-hydroxy-L-proline, trans-1-acetyl-4-hydroxy-L-proline, D-proline as well as L-citrulline and D,L-ornithine at 1 mM concentration do not inhibit the enzymatic conversion of L-glutamate Leishmania donovani
2.7.2.11 N-acetyl-L-proline 93% residual activity at 1 mM Leishmania donovani
2.7.2.11 trans-4-hydroxy-L-proline 89% residual activity at 1 mM Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.11 0.6
-
 ATP at pH 7.4 and 25°C Leishmania donovani
2.7.2.11 10
-
 L-glutamate at pH 7.4 and 25°C Leishmania donovani

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.11 Mg2+ 5 mM used in assay conditions Leishmania donovani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.11 105000
-
 gel filtration Leishmania donovani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.11 ATP + L-glutamate Leishmania donovani
-
 ADP + L-glutamate 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.11 Leishmania donovani A0A0R6Y3I5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.11 GST resin column chromatography Leishmania donovani

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.11 promastigote
-
 Leishmania donovani
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.11 ATP + glutaric acid 29% activity compared to L-glutamate Leishmania donovani ?
-
 ?
2.7.2.11 ATP + L-glutamate
-
 Leishmania donovani ADP + L-glutamate 5-phosphate
-
 ?
2.7.2.11 ATP + L-glutamate 100% activity Leishmania donovani ADP + L-glutamate 5-phosphate
-
 ?
2.7.2.11 additional information GTP, CTP or UTP do not replace ATP as phosphate donor. The enzyme has no activity with aminovaleric acid and 2-amino-4-phosphonobutyric acid Leishmania donovani ?
-
-

Subunits

EC Number Subunits Comment Organism
2.7.2.11 homotetramer 4 * 29000, SDS-PAGE Leishmania donovani
2.7.2.11 homotetramer 4 * 29033, calculated from amino acid sequence Leishmania donovani
2.7.2.11 homotetramer 4 * 29100, MALDI-TOF mass spectrometry Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
2.7.2.11 G5K
-
 Leishmania donovani
2.7.2.11 gamma-glutamyl kinase
-
 Leishmania donovani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.11 11.7
-
 ATP at pH 7.4 and 25°C Leishmania donovani
2.7.2.11 12.8
-
 L-glutamate at pH 7.4 and 25°C Leishmania donovani

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.11 43
-
 Mg2+ at pH 7.4 and 25°C Leishmania donovani

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.2.11 0.39
-
 at pH 7.4 and 25°C Leishmania donovani L-proline
2.7.2.11 0.49
-
 at pH 7.4 and 25°C Leishmania donovani 3,4-dehydro-L-proline
2.7.2.11 0.73
-
 at pH 7.4 and 25°C Leishmania donovani L-azetidine-2-carboxylic acid
2.7.2.11 1.8
-
 at pH 7.4 and 25°C Leishmania donovani L-4-thioproline

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.2.11 1.28
-
 L-glutamate at pH 7.4 and 25°C Leishmania donovani
2.7.2.11 19.5
-
 ATP at pH 7.4 and 25°C Leishmania donovani