Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Satomura, T.; Hayashi, J.; Ohshida, T.; Sakuraba, H.; Ohshima, T.; Suye, S.I.
    Enzymological characteristics of a novel archaeal dye-linked D-lactate dehydrogenase showing loose binding of FAD (2018), Extremophiles, 22, 975-981 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.6 expression in Escherichia coli Thermoproteus tenax

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.6 1.1
-
(R)-lactate pH 7.0, 60°C Thermoproteus tenax
1.1.99.6 1.1
-
D-lactate 60°C, pH not specified in the publication Thermoproteus tenax
1.1.99.6 4
-
(R)-lactate pH 7.0, 60°C Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.6 48000
-
SDS-PAGE Thermoproteus tenax
1.1.99.6 120000
-
gel filtration Thermoproteus tenax

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.6 Aeropyrum pernix Q9YEU4
-
-
1.1.99.6 Aeropyrum pernix ATCC 700893 Q9YEU4
-
-
1.1.99.6 Thermoproteus tenax G4RPF4
-
-
1.1.99.6 Thermoproteus tenax ATCC 35583 G4RPF4
-
-
1.1.99.6 Thermoproteus tenax DSM 2078 G4RPF4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.6
-
Thermoproteus tenax

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.6 0.85
-
pH 7.0, 60°C Thermoproteus tenax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.6 (R)-lactate + 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
-
Aeropyrum pernix pyruvate + ?
-
?
1.1.99.6 (R)-lactate + 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
-
Thermoproteus tenax pyruvate + ?
-
?
1.1.99.6 (R)-lactate + 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
-
Aeropyrum pernix ATCC 700893 pyruvate + ?
-
?
1.1.99.6 (R)-lactate + oxidized 2,6-dichlorophenolindophenol
-
Aeropyrum pernix pyruvate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.99.6 (R)-lactate + oxidized 2,6-dichlorophenolindophenol
-
Thermoproteus tenax pyruvate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.99.6 (R)-lactate + oxidized 2,6-dichlorophenolindophenol
-
Aeropyrum pernix ATCC 700893 pyruvate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.99.6 (R)-lactate + oxidized 2,6-dichlorophenolindophenol
-
Thermoproteus tenax DSM 2078 pyruvate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.99.6 (R)-lactate + oxidized 2,6-dichlorophenolindophenol
-
Thermoproteus tenax ATCC 35583 pyruvate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.99.6 (R)-lactate + phenazine methosulfate p-iodonitrotetrazolium
-
Aeropyrum pernix pyruvate + ?
-
?
1.1.99.6 (R)-lactate + phenazine methosulfate p-iodonitrotetrazolium
-
Aeropyrum pernix ATCC 700893 pyruvate + ?
-
?
1.1.99.6 D-lactate + 2,6-dichloroindophenol
-
Thermoproteus tenax pyruvate + reduced 2,6-dichloroindophenol
-
?
1.1.99.6 D-lactate + 2,6-dichloroindophenol
-
Thermoproteus tenax DSM 2078 pyruvate + reduced 2,6-dichloroindophenol
-
?
1.1.99.6 D-lactate + 2,6-dichloroindophenol
-
Thermoproteus tenax ATCC 35583 pyruvate + reduced 2,6-dichloroindophenol
-
?
1.1.99.6 D-lactate + 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
-
Thermoproteus tenax pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
-
?
1.1.99.6 D-lactate + 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
-
Thermoproteus tenax DSM 2078 pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
-
?
1.1.99.6 D-lactate + 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
-
Thermoproteus tenax ATCC 35583 pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
-
?
1.1.99.6 D-lactate + phenazine methosulfate p-iodonitrotetrazolium
-
Thermoproteus tenax pyruvate + reduced phenazine methosulfate p-iodonitrotetrazolium
-
?
1.1.99.6 additional information no acceptors: ferricyanide, benzyl viologen, and methylene blue Thermoproteus tenax ?
-
-
1.1.99.6 additional information D-glycerate, D-alanine, D-leucine, 2-hydroxyisobutyrate, L-lactate, L-alanine, and L-leucine are inert as the electron donor. Ferricyanide, benzyl viologen, and methylene blue are inert as electron acceptors Aeropyrum pernix ?
-
-
1.1.99.6 additional information D-glycerate, D-alanine, D-leucine, 2-hydroxyisobutyrate, L-lactate, L-alanine, and L-leucine are inert as the electron donor. Ferricyanide, benzyl viologen, and methylene blue are inert as electron acceptors Thermoproteus tenax ?
-
-
1.1.99.6 additional information D-glycerate, D-alanine, D-leucine, 2-hydroxyisobutyrate, L-lactate, L-alanine, and L-leucine are inert as the electron donor. Ferricyanide, benzyl viologen, and methylene blue are inert as electron acceptors Aeropyrum pernix ATCC 700893 ?
-
-
1.1.99.6 additional information no acceptors: ferricyanide, benzyl viologen, and methylene blue Thermoproteus tenax DSM 2078 ?
-
-
1.1.99.6 additional information D-glycerate, D-alanine, D-leucine, 2-hydroxyisobutyrate, L-lactate, L-alanine, and L-leucine are inert as the electron donor. Ferricyanide, benzyl viologen, and methylene blue are inert as electron acceptors Thermoproteus tenax DSM 2078 ?
-
-
1.1.99.6 additional information no acceptors: ferricyanide, benzyl viologen, and methylene blue Thermoproteus tenax ATCC 35583 ?
-
-
1.1.99.6 additional information D-glycerate, D-alanine, D-leucine, 2-hydroxyisobutyrate, L-lactate, L-alanine, and L-leucine are inert as the electron donor. Ferricyanide, benzyl viologen, and methylene blue are inert as electron acceptors Thermoproteus tenax ATCC 35583 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.1.99.6 homodimer 2 * 48000, SDS-PAGE Thermoproteus tenax

Synonyms

EC Number Synonyms Comment Organism
1.1.99.6 dye-DLDH
-
Aeropyrum pernix
1.1.99.6 dye-DLDH
-
Thermoproteus tenax
1.1.99.6 dye-linked D-lactate dehydrogenase
-
Aeropyrum pernix
1.1.99.6 dye-linked D-lactate dehydrogenase
-
Thermoproteus tenax
1.1.99.6 TTX_0794
-
Thermoproteus tenax

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.6 80
-
-
Thermoproteus tenax
1.1.99.6 80
-
above 80°C Aeropyrum pernix
1.1.99.6 80
-
above 80°C Thermoproteus tenax

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.6 80
-
10 min below 80°C, 80% residual activity Thermoproteus tenax
1.1.99.6 80
-
enzyme retains full activity after incubation for 10 min Aeropyrum pernix
1.1.99.6 80
-
enzyme retains full activity after incubation for 10 min Thermoproteus tenax
1.1.99.6 100
-
10 min, loss of activity Thermoproteus tenax

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.6 7
-
-
Thermoproteus tenax
1.1.99.6 7.5
-
-
Aeropyrum pernix

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.99.6 4 10 50°C, 30 min, more than 70% residual activity Thermoproteus tenax
1.1.99.6 4 11 the enzyme retains more than 70% activity after incubation at 50°C for 30 min Aeropyrum pernix
1.1.99.6 4 11 the enzyme retains more than 70% activity after incubation at 50°C for 30 min Thermoproteus tenax

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.6 FAD
-
Thermoproteus tenax
1.1.99.6 FAD reversibly binds FAD. Loose binding of FAD Aeropyrum pernix
1.1.99.6 FAD reversibly binds FAD. Loose binding of FAD Thermoproteus tenax