EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.99.6 | expression in Escherichia coli | Thermoproteus tenax |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.6 | 1.1 | - |
(R)-lactate | pH 7.0, 60°C | Thermoproteus tenax | |
1.1.99.6 | 1.1 | - |
D-lactate | 60°C, pH not specified in the publication | Thermoproteus tenax | |
1.1.99.6 | 4 | - |
(R)-lactate | pH 7.0, 60°C | Aeropyrum pernix |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.99.6 | 48000 | - |
SDS-PAGE | Thermoproteus tenax |
1.1.99.6 | 120000 | - |
gel filtration | Thermoproteus tenax |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.6 | Aeropyrum pernix | Q9YEU4 | - |
- |
1.1.99.6 | Aeropyrum pernix ATCC 700893 | Q9YEU4 | - |
- |
1.1.99.6 | Thermoproteus tenax | G4RPF4 | - |
- |
1.1.99.6 | Thermoproteus tenax ATCC 35583 | G4RPF4 | - |
- |
1.1.99.6 | Thermoproteus tenax DSM 2078 | G4RPF4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.6 | - |
Thermoproteus tenax |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.6 | 0.85 | - |
pH 7.0, 60°C | Thermoproteus tenax |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.6 | (R)-lactate + 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | - |
Aeropyrum pernix | pyruvate + ? | - |
? | |
1.1.99.6 | (R)-lactate + 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | - |
Thermoproteus tenax | pyruvate + ? | - |
? | |
1.1.99.6 | (R)-lactate + 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | - |
Aeropyrum pernix ATCC 700893 | pyruvate + ? | - |
? | |
1.1.99.6 | (R)-lactate + oxidized 2,6-dichlorophenolindophenol | - |
Aeropyrum pernix | pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.6 | (R)-lactate + oxidized 2,6-dichlorophenolindophenol | - |
Thermoproteus tenax | pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.6 | (R)-lactate + oxidized 2,6-dichlorophenolindophenol | - |
Aeropyrum pernix ATCC 700893 | pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.6 | (R)-lactate + oxidized 2,6-dichlorophenolindophenol | - |
Thermoproteus tenax DSM 2078 | pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.6 | (R)-lactate + oxidized 2,6-dichlorophenolindophenol | - |
Thermoproteus tenax ATCC 35583 | pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.6 | (R)-lactate + phenazine methosulfate p-iodonitrotetrazolium | - |
Aeropyrum pernix | pyruvate + ? | - |
? | |
1.1.99.6 | (R)-lactate + phenazine methosulfate p-iodonitrotetrazolium | - |
Aeropyrum pernix ATCC 700893 | pyruvate + ? | - |
? | |
1.1.99.6 | D-lactate + 2,6-dichloroindophenol | - |
Thermoproteus tenax | pyruvate + reduced 2,6-dichloroindophenol | - |
? | |
1.1.99.6 | D-lactate + 2,6-dichloroindophenol | - |
Thermoproteus tenax DSM 2078 | pyruvate + reduced 2,6-dichloroindophenol | - |
? | |
1.1.99.6 | D-lactate + 2,6-dichloroindophenol | - |
Thermoproteus tenax ATCC 35583 | pyruvate + reduced 2,6-dichloroindophenol | - |
? | |
1.1.99.6 | D-lactate + 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | - |
Thermoproteus tenax | pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | - |
? | |
1.1.99.6 | D-lactate + 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | - |
Thermoproteus tenax DSM 2078 | pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | - |
? | |
1.1.99.6 | D-lactate + 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | - |
Thermoproteus tenax ATCC 35583 | pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | - |
? | |
1.1.99.6 | D-lactate + phenazine methosulfate p-iodonitrotetrazolium | - |
Thermoproteus tenax | pyruvate + reduced phenazine methosulfate p-iodonitrotetrazolium | - |
? | |
1.1.99.6 | additional information | no acceptors: ferricyanide, benzyl viologen, and methylene blue | Thermoproteus tenax | ? | - |
- |
|
1.1.99.6 | additional information | D-glycerate, D-alanine, D-leucine, 2-hydroxyisobutyrate, L-lactate, L-alanine, and L-leucine are inert as the electron donor. Ferricyanide, benzyl viologen, and methylene blue are inert as electron acceptors | Aeropyrum pernix | ? | - |
- |
|
1.1.99.6 | additional information | D-glycerate, D-alanine, D-leucine, 2-hydroxyisobutyrate, L-lactate, L-alanine, and L-leucine are inert as the electron donor. Ferricyanide, benzyl viologen, and methylene blue are inert as electron acceptors | Thermoproteus tenax | ? | - |
- |
|
1.1.99.6 | additional information | D-glycerate, D-alanine, D-leucine, 2-hydroxyisobutyrate, L-lactate, L-alanine, and L-leucine are inert as the electron donor. Ferricyanide, benzyl viologen, and methylene blue are inert as electron acceptors | Aeropyrum pernix ATCC 700893 | ? | - |
- |
|
1.1.99.6 | additional information | no acceptors: ferricyanide, benzyl viologen, and methylene blue | Thermoproteus tenax DSM 2078 | ? | - |
- |
|
1.1.99.6 | additional information | D-glycerate, D-alanine, D-leucine, 2-hydroxyisobutyrate, L-lactate, L-alanine, and L-leucine are inert as the electron donor. Ferricyanide, benzyl viologen, and methylene blue are inert as electron acceptors | Thermoproteus tenax DSM 2078 | ? | - |
- |
|
1.1.99.6 | additional information | no acceptors: ferricyanide, benzyl viologen, and methylene blue | Thermoproteus tenax ATCC 35583 | ? | - |
- |
|
1.1.99.6 | additional information | D-glycerate, D-alanine, D-leucine, 2-hydroxyisobutyrate, L-lactate, L-alanine, and L-leucine are inert as the electron donor. Ferricyanide, benzyl viologen, and methylene blue are inert as electron acceptors | Thermoproteus tenax ATCC 35583 | ? | - |
- |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.99.6 | homodimer | 2 * 48000, SDS-PAGE | Thermoproteus tenax |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.6 | dye-DLDH | - |
Aeropyrum pernix |
1.1.99.6 | dye-DLDH | - |
Thermoproteus tenax |
1.1.99.6 | dye-linked D-lactate dehydrogenase | - |
Aeropyrum pernix |
1.1.99.6 | dye-linked D-lactate dehydrogenase | - |
Thermoproteus tenax |
1.1.99.6 | TTX_0794 | - |
Thermoproteus tenax |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.6 | 80 | - |
- |
Thermoproteus tenax |
1.1.99.6 | 80 | - |
above 80°C | Aeropyrum pernix |
1.1.99.6 | 80 | - |
above 80°C | Thermoproteus tenax |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.6 | 80 | - |
10 min below 80°C, 80% residual activity | Thermoproteus tenax |
1.1.99.6 | 80 | - |
enzyme retains full activity after incubation for 10 min | Aeropyrum pernix |
1.1.99.6 | 80 | - |
enzyme retains full activity after incubation for 10 min | Thermoproteus tenax |
1.1.99.6 | 100 | - |
10 min, loss of activity | Thermoproteus tenax |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.6 | 7 | - |
- |
Thermoproteus tenax |
1.1.99.6 | 7.5 | - |
- |
Aeropyrum pernix |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.6 | 4 | 10 | 50°C, 30 min, more than 70% residual activity | Thermoproteus tenax |
1.1.99.6 | 4 | 11 | the enzyme retains more than 70% activity after incubation at 50°C for 30 min | Aeropyrum pernix |
1.1.99.6 | 4 | 11 | the enzyme retains more than 70% activity after incubation at 50°C for 30 min | Thermoproteus tenax |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.6 | FAD | - |
Thermoproteus tenax | |
1.1.99.6 | FAD | reversibly binds FAD. Loose binding of FAD | Aeropyrum pernix | |
1.1.99.6 | FAD | reversibly binds FAD. Loose binding of FAD | Thermoproteus tenax |