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Literature summary extracted from
- NADP+-dependent isocitrate dehydrogenase from a psychrophilic bacterium, Psychromonas marina (2017), Extremophiles, 21, 711-721 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.42 | expressed in Escherichia coli strain DEK2004 | Psychromonas marina |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.42 | 0.03353 | - |
isocitrate | at pH 8.0 and 20°C | Psychromonas marina |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.42 | Mg2+ | 0.67 mM used in assay conditions | Psychromonas marina | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.42 | isocitrate + NADP+ | Psychromonas marina | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.42 | Psychromonas marina | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.42 | Ni-NTA agarose column chromatography | Psychromonas marina |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.42 | 136.03 | - |
His-tagged enzyme, at pH 8.0 and 35°C | Psychromonas marina |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.42 | isocitrate + NADP+ | - |
Psychromonas marina | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
| 1.1.1.42 | additional information | no activity Is detected when 0.12 mM NAD+, instead of NADP+, is added to the reaction mixture | Psychromonas marina | ? | - |
- |
|
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.42 | monomer | 1 * 80000, SDS-PAGE | Psychromonas marina |
| 1.1.1.42 | monomer | 1 * 80426, calculated from amino acid sequence | Psychromonas marina |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.42 | IDH | - |
Psychromonas marina |
| 1.1.1.42 | NADP+-dependent isocitrate dehydrogenase | - |
Psychromonas marina |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.42 | 35 | - |
- |
Psychromonas marina |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.42 | 30 | - |
the enzyme loses 74% of the activity after incubation for 10 min at 30°C | Psychromonas marina |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.42 | 102.98 | - |
isocitrate | at pH 8.0 and 20°C | Psychromonas marina | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.42 | 7.5 | 8 | - |
Psychromonas marina |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.42 | NADP+ | - |
Psychromonas marina | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.42 | 3086 | - |
isocitrate | at pH 8.0 and 20°C | Psychromonas marina | |
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Release 2023.1 (January 2023)
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