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Literature summary extracted from

  • Wohlfarth, G.; Geerligs, G.; Diekert, G.
    Purification and properties of a NADH-dependent 5,10-methylenetetrahydrofolate reductase from Peptostreptococcus productus (1990), Eur. J. Biochem., 192, 411-417 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.54 0.01
-
 NADH pH 5.5, 37°C Blautia producta

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.54 membrane associated to membrane Blautia producta 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.54 250000
-
 gel filtration Blautia producta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.54 5,10-methylenetetrahydrofolate + NADH + H+ Blautia producta
-
 5-methyltetrahydrofolate + NAD+
-
 ?
1.5.1.54 5,10-methylenetetrahydrofolate + NADH + H+ Blautia producta Marburg
-
 5-methyltetrahydrofolate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.54 Blautia producta
-
-
-
1.5.1.54 Blautia producta Marburg
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.54
-
 Blautia producta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.54 380
-
 pH 5.5, 37°C Blautia producta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.54 5,10-methylenetetrahydrofolate + NADH + H+
-
 Blautia producta 5-methyltetrahydrofolate + NAD+
-
 ?
1.5.1.54 5,10-methylenetetrahydrofolate + NADH + H+
-
 Blautia producta Marburg 5-methyltetrahydrofolate + NAD+
-
 ?
1.5.1.54 5,10-methylenetetrahydrofolate + reduced methylene blue
-
 Blautia producta 5-methyltetrahydrofolate + methylene blue
-
 r
1.5.1.54 5,10-methylenetetrahydrofolate + reduced methylene blue
-
 Blautia producta Marburg 5-methyltetrahydrofolate + methylene blue
-
 r
1.5.1.54 5-methyltetrahydrofolate + methylene blue
-
 Blautia producta 5,10-methylenetetrahydrofolate + reduced methylene blue
-
 r
1.5.1.54 5-methyltetrahydrofolate + methylene blue
-
 Blautia producta Marburg 5,10-methylenetetrahydrofolate + reduced methylene blue
-
 r
1.5.1.54 additional information the enzyme catalyzes the methylenetetrahydrofolate reduction with methylene blue as an artificial electron donor. The oxidation of methyltetrahydrofolate is mediated with methylene blue as the electron acceptor. Neither NAD+ nor viologen dyes can replace methylene blue in this reaction Blautia producta ?
-
-
1.5.1.54 additional information the enzyme catalyzes the methylenetetrahydrofolate reduction with methylene blue as an artificial electron donor. The oxidation of methyltetrahydrofolate is mediated with methylene blue as the electron acceptor. Neither NAD+ nor viologen dyes can replace methylene blue in this reaction Blautia producta Marburg ?
-
-

Subunits

EC Number Subunits Comment Organism
1.5.1.54 octamer 8 * 32000, SDS-PAGE Blautia producta

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.54 FAD 4 FAD/mol octamer Blautia producta
1.5.1.54 additional information no cosubstrates: NADPH, FAD(H2) Blautia producta
1.5.1.54 NADH
-
 Blautia producta