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Literature summary extracted from

  • Wang, Y.; Xiao, D.; Liu, Q.; Zhang, Y.; Hu, C.; Sun, J.; Yang, C.; Xu, P.; Ma, C.; Gao, C.
    Two NAD-independent l-lactate dehydrogenases drive L-lactate utilization in Pseudomonas aeruginosa PAO1 (2018), Environ. Microbiol. Rep., 10, 569-575 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.2.3 medicine both LldD and LldA are important for growth of Pseudomonas aeruginosa PAO1 in synthetic cystic fibrosis sputum medium Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.3 expression in Escherichia coli Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.3 0.336
-
 L-lactate pH 7.4, temperature not specified in the publication Pseudomonas aeruginosa
1.1.2.3 1.102
-
 L-lactate pH 7.4, temperature not specified in the publication Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.3 membrane
-
 Pseudomonas aeruginosa 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Pseudomonas aeruginosa Q9HV37
-
-
1.1.2.3 Pseudomonas aeruginosa Q9I197
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 L-lactate + 2 ferricytochrome c
-
 Pseudomonas aeruginosa pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.2.3 lldA
-
 Pseudomonas aeruginosa
1.1.2.3 LldD
-
 Pseudomonas aeruginosa
1.1.2.3 PA4771
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.3 FMN
-
 Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
1.1.2.3 physiological function disruption of the lldD gene impairs the growth of this strain in L-lactate, but the mutant strain is still able to grow after a long lag phase. Deletion of both the LldD and LldA genes completely impairs the L-lactate utilization capacity of the strain Pseudomonas aeruginosa
1.1.2.3 physiological function loss of LldA has little effect on L-lactate utilization. Deletion of both the LldD and LldA genes completely impairs the L-lactate utilization capacity of the strain Pseudomonas aeruginosa