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Literature summary extracted from

  • An, J.; Nie, Y.; Xu, Y.
    Structural insights into alcohol dehydrogenases catalyzing asymmetric reductions (2019), Crit. Rev. Biotechnol., 39, 366-379 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.71 hexane in asymmetric reduction by TeSADH in pure hexane, the organic solvent makes the process more efficient by allowing high concentrations of substrates to be used Thermoanaerobacter ethanolicus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.71 G198D site-directed mutagenesis, the variant of the NAD(P)H-dependent ADH, TeSADH, shows a significant switch of cofactor dependence from NADPH towards NADH compared to the wild-type enzyme Thermoanaerobacter ethanolicus
1.1.1.71 W110A site-directed mutagenesis, the mutant is able not only to reduce phenyl-ring-containing ketones with high enantioselectivity, but it can also racemize the corresponding enantiopure alcohols Thermoanaerobacter ethanolicus
1.1.1.71 W110A/I86A site-directed mutagenesis, the mutant of TeSADH reduces phenyl-ring-containing ketones with high enantioselectivity and racemizes the corresponding enantiopure alcohols, and it expands substrate specificity to accommodate ketones bearing two sterically demanding groups Thermoanaerobacter ethanolicus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.71 hexane in asymmetric reduction by TeSADH in pure hexane, the organic solvent makes the process more efficient by allowing high concentrations of substrates to be used Thermoanaerobacter ethanolicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.71 Thermoanaerobacter ethanolicus
-
-
-

Synonyms

EC Number Synonyms Comment Organism
1.1.1.71 NAD(P)H-dependent ADH
-
 Thermoanaerobacter ethanolicus
1.1.1.71 TeSADH
-
 Thermoanaerobacter ethanolicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.71 NAD+
-
 Thermoanaerobacter ethanolicus
1.1.1.71 NADH
-
 Thermoanaerobacter ethanolicus
1.1.1.71 NADP+
-
 Thermoanaerobacter ethanolicus
1.1.1.71 NADPH
-
 Thermoanaerobacter ethanolicus

General Information

EC Number General Information Comment Organism
1.1.1.71 evolution ADHs structure and function comparisons, SDR family enzymes structure-function comparisons, overview Thermoanaerobacter ethanolicus