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Literature summary extracted from

  • Piper, P.; Begres, B.; Snider, M.; Fraga, D.
    Two cryptosporidia species encode active creatine kinases that are not seen in other apicomplexa species (2020), Comp. Biochem. Physiol. B Biochem. Mol. Biol., 246-247, 110459 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.2 expressed in Escherichia coli BL21(DE3) cells Cryptosporidium andersoni
2.7.3.2 expressed in Escherichia coli BL21(DE3) cells Cryptosporidium muris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.3.2 ATP
-
 Cryptosporidium muris
2.7.3.2 Creatine
-
 Cryptosporidium muris
2.7.3.2 glycocyamine
-
 Cryptosporidium muris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.2 0.8
-
 ATP in form of Mg-ATP, at pH 8.5 and 25°C Cryptosporidium muris
2.7.3.2 1.7
-
 ATP in form of Mg-ATP, at pH 8.5 and 25°C Cryptosporidium muris
2.7.3.2 2
-
 Creatine at pH 8.5 and 25°C Cryptosporidium muris
2.7.3.2 6.7
-
 glycocyamine at pH 8.5 and 25°C Cryptosporidium muris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.2 90000
-
 gel filtration Cryptosporidium muris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.3.2 ATP + creatine Cryptosporidium muris
-
 ADP + phosphocreatine
-
 ?
2.7.3.2 ATP + creatine Cryptosporidium andersoni
-
 ADP + phosphocreatine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Cryptosporidium andersoni
-
-
-
2.7.3.2 Cryptosporidium andersoni A0A1J4MR22
-
-
2.7.3.2 Cryptosporidium muris
-
-
-
2.7.3.2 Cryptosporidium muris B6AJN7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.2 Ni-affinity column chromatography Cryptosporidium andersoni
2.7.3.2 Ni-affinity column chromatography Cryptosporidium muris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ATP + creatine
-
 Cryptosporidium muris ADP + phosphocreatine
-
 ?
2.7.3.2 ATP + creatine
-
 Cryptosporidium andersoni ADP + phosphocreatine
-
 ?
2.7.3.2 ATP + creatine the enzyme has a 20fold greater preference for creatine compared to glycocyamine Cryptosporidium muris ADP + phosphocreatine
-
 ?
2.7.3.2 ATP + creatine the enzyme shows a 20fold greater preference for creatine than glycocyamine Cryptosporidium muris ADP + phosphocreatine
-
 ?
2.7.3.2 ATP + glycocyamine
-
 Cryptosporidium muris ADP + phosphoglycocyamine
-
 ?
2.7.3.2 ATP + glycocyamine
-
 Cryptosporidium andersoni ADP + phosphoglycocyamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.3.2 homodimer 2 * 45000, SDS-PAGE Cryptosporidium muris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.3.2 0.5
-
 glycocyamine at pH 8.5 and 25°C Cryptosporidium muris
2.7.3.2 30
-
 Creatine pH and temperature not specified in the publication Cryptosporidium muris
2.7.3.2 30
-
 Creatine at pH 8.5 and 25°C Cryptosporidium muris

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.3.2 ATP
-
 Cryptosporidium andersoni
2.7.3.2 ATP
-
 Cryptosporidium muris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.3.2 0.13
-
 ATP in form of Mg-ATP, with creatine as substrate, at pH 8.5 and 25°C Cryptosporidium muris
2.7.3.2 0.3
-
 ATP in form of Mg-ATP, with glycocyamine as substrate, at pH 8.5 and 25°C Cryptosporidium muris
2.7.3.2 0.33
-
 Creatine at pH 8.5 and 25°C Cryptosporidium muris
2.7.3.2 1.2
-
 glycocyamine at pH 8.5 and 25°C Cryptosporidium muris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.3.2 0.8
-
 glycocyamine at pH 8.5 and 25°C Cryptosporidium muris
2.7.3.2 15.4
-
 Creatine pH and temperature not specified in the publication Cryptosporidium muris
2.7.3.2 15.4
-
 Creatine at pH 8.5 and 25°C Cryptosporidium muris