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Literature summary extracted from

  • Pulido, S.A.; Nguyen, V.H.; Alzate, J.F.; Cedeno, D.L.; Makurath, M.A.; Rios-Vasquez, A.; Duque-Benitez, S.M.; Jones, M.A.; Robledo, S.M.; Friesen, J.A.
    Insights into the phosphatidylcholine and phosphatidylethanolamine biosynthetic pathways in Leishmania parasites and characterization of a choline kinase from Leishmania infantum (2017), Comp. Biochem. Physiol. B, 213, 45-54 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.32 expressed in Escherichia coli BL21(DE3) cells Leishmania infantum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.32 N,N,N-trimethyl-N-(5,5-diphenylpent-4-en-1-yl)ammonium iodide
-
 Leishmania infantum
2.7.1.32 N,N,N-trimethyl-N-(6,6-diphenylhex-5-en-1-yl)ammonium iodide
-
 Leishmania infantum
2.7.1.32 N-iodomethyl-N,N-dimethyl-N-(4,4-diphenylbut-3-en-1-yl)ammonium iodide
-
 Leishmania infantum
2.7.1.32 N-iodomethyl-N,N-dimethyl-N-(5,5-diphenylpent-4-en-1-yl)ammonium iodide
-
 Leishmania infantum
2.7.1.32 N-iodomethyl-N,N-dimethyl-N-(6,6-diphenylhex-5-en-1-yl)ammonium iodide
-
 Leishmania infantum
2.7.1.32 N-iodomethylcholine iodide
-
 Leishmania infantum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.32 0.089
-
 choline at pH 9.0 and 37°C Leishmania infantum
2.7.1.32 75
-
 ethanolamine at pH 9.0 and 37°C Leishmania infantum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.32 Mg2+ 11 mM used in assay conditions Leishmania infantum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Leishmania infantum A4IB50
-
-
2.7.1.32 Leishmania infantum JPCM5 A4IB50
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.32 Ni-NTA resin column chromatography Leishmania infantum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.32 promastigote
-
 Leishmania infantum
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.32 -20°C, 50 mM Tris and 50 mM glycine pH 9.0, 24 h, 30-50% loss of activity Leishmania infantum
2.7.1.32 -80°C, 50 mM Tris and 50 mM glycine pH 9.0, with or without freeze drying, 24 h, about 50% loss of activity Leishmania infantum
2.7.1.32 4°C, 50 mM Tris and 50 mM glycine pH 9.0, 24 h, no loss of activity Leishmania infantum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 ATP + choline the catalytic efficiency (Vmax/Km) using choline as substrate is 2000fold greater than the efficiency using ethanolamine as substrate Leishmania infantum ADP + O-phosphocholine
-
 ?
2.7.1.32 ATP + choline the catalytic efficiency (Vmax/Km) using choline as substrate is 2000fold greater than the efficiency using ethanolamine as substrate Leishmania infantum JPCM5 ADP + O-phosphocholine
-
 ?
2.7.1.32 ATP + ethanolamine
-
 Leishmania infantum ADP + O-phosphoethanolamine
-
 ?
2.7.1.32 ATP + ethanolamine
-
 Leishmania infantum JPCM5 ADP + O-phosphoethanolamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.32 ? x * 79000, SDS-PAGE Leishmania infantum

Synonyms

EC Number Synonyms Comment Organism
2.7.1.32 LinfC/EK35 the enzyme has choline kinase and ethanolamine kinase activities Leishmania infantum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.32 9
-
-
 Leishmania infantum