Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yu, R.; Kim, Y.; Maltseva, N.; Braunstein, P.; Joachimiak, A.; Hedstrom, L.
    Oxanosine monophosphate is a covalent inhibitor of inosine 5'-monophosphate dehydrogenase (2019), Chem. Res. Toxicol., 32, 456-466 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.205 expressed in Escherichia coli BL21 cells Cryptosporidium parvum
1.1.1.205 expressed in Escherichia coli BL21 cells Homo sapiens
1.1.1.205 expressed in Escherichia coli BL21 cells Tritrichomonas foetus
1.1.1.205 expressed in Escherichia coli BL21 cells Bacillus anthracis
1.1.1.205 expressed in Escherichia coli BL21 cells Campylobacter jejuni

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.205 enzyme in complex with oxanosine monophosphate, sitting drop vapor diffusion method, using 0.1 M lithium sulfate, 0.1 MES, pH 6.0, and 35% (v/v) 2-methyl-2,4-pentanediol Campylobacter jejuni
1.1.1.205 enzyme in complex with oxanosine monophosphate, sitting drop vapor diffusion method, using 0.1 M magnesium chloride, 0.1 M MES, pH 6.5, and 30% (v/v) PEG 400 Bacillus anthracis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.205 GMP
-
 Bacillus anthracis
1.1.1.205 GMP
-
 Campylobacter jejuni
1.1.1.205 GMP
-
 Cryptosporidium parvum
1.1.1.205 GMP
-
 Homo sapiens
1.1.1.205 GMP
-
 Tritrichomonas foetus
1.1.1.205 oxanosine monophosphate potent reversible competitive inhibitor Bacillus anthracis
1.1.1.205 oxanosine monophosphate potent reversible competitive inhibitor Campylobacter jejuni
1.1.1.205 oxanosine monophosphate potent reversible competitive inhibitor Cryptosporidium parvum
1.1.1.205 oxanosine monophosphate potent reversible competitive inhibitor Homo sapiens
1.1.1.205 oxanosine monophosphate potent reversible competitive inhibitor Tritrichomonas foetus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.205 0.0033
-
 IMP at pH 8.0 and 25°C Homo sapiens
1.1.1.205 0.0063
-
 IMP at pH 8.0 and 25°C Tritrichomonas foetus
1.1.1.205 0.007
-
 NAD+ at pH 8.0 and 25°C Homo sapiens
1.1.1.205 0.01
-
 IMP at pH 8.0 and 25°C Cryptosporidium parvum
1.1.1.205 0.012
-
 IMP at pH 8.0 and 25°C Campylobacter jejuni
1.1.1.205 0.045
-
 NAD+ at pH 8.0 and 25°C Cryptosporidium parvum
1.1.1.205 0.066
-
 NAD+ at pH 8.0 and 25°C Campylobacter jejuni
1.1.1.205 0.08
-
 NAD+ at pH 8.0 and 25°C Tritrichomonas foetus
1.1.1.205 0.083
-
 IMP at pH 8.0 and 25°C Bacillus anthracis
1.1.1.205 0.7
-
 NAD+ at pH 8.0 and 25°C Bacillus anthracis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.205 IMP + NAD+ + H2O Cryptosporidium parvum
-
 XMP + NADH + H+
-
 ?
1.1.1.205 IMP + NAD+ + H2O Homo sapiens
-
 XMP + NADH + H+
-
 ?
1.1.1.205 IMP + NAD+ + H2O Tritrichomonas foetus
-
 XMP + NADH + H+
-
 ?
1.1.1.205 IMP + NAD+ + H2O Bacillus anthracis
-
 XMP + NADH + H+
-
 ?
1.1.1.205 IMP + NAD+ + H2O Campylobacter jejuni
-
 XMP + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.205 Bacillus anthracis A0A6L8P2U9
-
-
1.1.1.205 Campylobacter jejuni A0A2R4D3F6
-
-
1.1.1.205 Cryptosporidium parvum
-
-
-
1.1.1.205 Homo sapiens P12268
-
-
1.1.1.205 Tritrichomonas foetus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.205 Ni-NTA Sepharose bead chromatography and Superdex 200 gel filtration Cryptosporidium parvum
1.1.1.205 Ni-NTA Sepharose bead chromatography and Superdex 200 gel filtration Homo sapiens
1.1.1.205 Ni-NTA Sepharose bead chromatography and Superdex 200 gel filtration Tritrichomonas foetus
1.1.1.205 Ni-NTA Sepharose bead chromatography and Superdex 200 gel filtration Bacillus anthracis
1.1.1.205 Ni-NTA Sepharose bead chromatography and Superdex 200 gel filtration Campylobacter jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.205 IMP + NAD+ + H2O
-
 Cryptosporidium parvum XMP + NADH + H+
-
 ?
1.1.1.205 IMP + NAD+ + H2O
-
 Homo sapiens XMP + NADH + H+
-
 ?
1.1.1.205 IMP + NAD+ + H2O
-
 Tritrichomonas foetus XMP + NADH + H+
-
 ?
1.1.1.205 IMP + NAD+ + H2O
-
 Bacillus anthracis XMP + NADH + H+
-
 ?
1.1.1.205 IMP + NAD+ + H2O
-
 Campylobacter jejuni XMP + NADH + H+
-
 ?
1.1.1.205 additional information the enzyme does not hydrolyze oxanosine monophosphate Cryptosporidium parvum ?
-
-
1.1.1.205 additional information the enzyme does not hydrolyze oxanosine monophosphate Homo sapiens ?
-
-
1.1.1.205 additional information the enzyme does not hydrolyze oxanosine monophosphate Tritrichomonas foetus ?
-
-
1.1.1.205 additional information the enzyme does not hydrolyze oxanosine monophosphate Bacillus anthracis ?
-
-
1.1.1.205 additional information the enzyme does not hydrolyze oxanosine monophosphate Campylobacter jejuni ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.1.1.205 IMPDH
-
 Cryptosporidium parvum
1.1.1.205 IMPDH
-
 Tritrichomonas foetus
1.1.1.205 IMPDH
-
 Bacillus anthracis
1.1.1.205 IMPDH
-
 Campylobacter jejuni
1.1.1.205 IMPDH2 isoform Homo sapiens
1.1.1.205 inosine 5'-monophosphate dehydrogenase
-
 Cryptosporidium parvum
1.1.1.205 inosine 5'-monophosphate dehydrogenase
-
 Homo sapiens
1.1.1.205 inosine 5'-monophosphate dehydrogenase
-
 Tritrichomonas foetus
1.1.1.205 inosine 5'-monophosphate dehydrogenase
-
 Bacillus anthracis
1.1.1.205 inosine 5'-monophosphate dehydrogenase
-
 Campylobacter jejuni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.205 0.22
-
 NAD+ at pH 8.0 and 25°C Homo sapiens
1.1.1.205 0.22
-
 IMP at pH 8.0 and 25°C Homo sapiens
1.1.1.205 0.7
-
 NAD+ at pH 8.0 and 25°C Tritrichomonas foetus
1.1.1.205 0.7
-
 IMP at pH 8.0 and 25°C Tritrichomonas foetus
1.1.1.205 2.2
-
 NAD+ at pH 8.0 and 25°C Campylobacter jejuni
1.1.1.205 2.2
-
 IMP at pH 8.0 and 25°C Campylobacter jejuni
1.1.1.205 3
-
 NAD+ at pH 8.0 and 25°C Bacillus anthracis
1.1.1.205 3
-
 IMP at pH 8.0 and 25°C Bacillus anthracis
1.1.1.205 3.2
-
 NAD+ at pH 8.0 and 25°C Cryptosporidium parvum
1.1.1.205 3.2
-
 IMP at pH 8.0 and 25°C Cryptosporidium parvum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.205 NAD+
-
 Cryptosporidium parvum
1.1.1.205 NAD+
-
 Homo sapiens
1.1.1.205 NAD+
-
 Tritrichomonas foetus
1.1.1.205 NAD+
-
 Bacillus anthracis
1.1.1.205 NAD+
-
 Campylobacter jejuni

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.205 0.000013
-
 oxanosine monophosphate at pH 8.0 and 25°C Homo sapiens
1.1.1.205 0.000017
-
 oxanosine monophosphate at pH 8.0 and 25°C Tritrichomonas foetus
1.1.1.205 0.000051
-
 oxanosine monophosphate at pH 8.0 and 25°C Campylobacter jejuni
1.1.1.205 0.00009
-
 oxanosine monophosphate at pH 8.0 and 25°C Cryptosporidium parvum
1.1.1.205 0.00034
-
 oxanosine monophosphate at pH 8.0 and 25°C Bacillus anthracis
1.1.1.205 0.008
-
 GMP at pH 8.0 and 25°C Campylobacter jejuni
1.1.1.205 0.021
-
 GMP at pH 8.0 and 25°C Cryptosporidium parvum
1.1.1.205 0.024
-
 GMP at pH 8.0 and 25°C Tritrichomonas foetus
1.1.1.205 0.037
-
 GMP at pH 8.0 and 25°C Homo sapiens
1.1.1.205 0.115
-
 GMP at pH 8.0 and 25°C Bacillus anthracis