Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Seo, E.J.; Kim, H.J.; Kim, M.J.; Kim, J.S.; Park, J.B.
    Cofactor specificity engineering of a long-chain secondary alcohol dehydrogenase from Micrococcus luteus for redox-neutral biotransformation of fatty acids (2019), Chem. Commun. (Camb.), 55, 14462-14465 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.192 expressed in Escherichia coli BL21(DE3) cells Micrococcus luteus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.192 hanging drop vapor diffusion method, using 1.0 M trisodium citrate dihydrate at pH 6.5 Micrococcus luteus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.192 D37S/A38R/V39S the mutant has preferred activity with NADP+ and shows increased catalytic efficiency compared to the wild type enzyme Micrococcus luteus
1.1.1.192 D37S/V39S the mutant has also activity with NADP+ and shows increased catalytic efficiency compared to the wild type enzyme Micrococcus luteus
1.1.1.192 T15I/D37S/A38R/V39S the mutant shows about 10fold increased catalytic efficiency with 10-hydroxyoctadecanoic acid and has 1800fold increase in catalytic efficiency for NADP+ compared to the wild type enzyme Micrococcus luteus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.192 Micrococcus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.192 Ni-NTA column chromatography Micrococcus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.192 10,12-dihydroxyoctadecanoic acid + NAD+ + H2O
-
 Micrococcus luteus ?
-
 ?
1.1.1.192 10,12-dihydroxyoctadecanoic acid + NADP+ + H2O the reaction is catalyzed by mutant enzyme D37S/V39S Micrococcus luteus ? + NADPH
-
 ?
1.1.1.192 10-hydroxyoctadecanoic acid + NAD+ + H2O
-
 Micrococcus luteus 9-(nonanoyloxy)nonanoic acid + NADH
-
 ?
1.1.1.192 10-hydroxyoctadecanoic acid + NADP+ + H2O the reaction is catalyzed by mutant enzyme D37S/A38R/V39S/T15I Micrococcus luteus 9-(nonanoyloxy)nonanoic acid + NADPH
-
 ?
1.1.1.192 12-hydroxyoctadecanoic acid + NAD+ + H2O
-
 Micrococcus luteus ? + NADH
-
 ?
1.1.1.192 4-decanol + NAD+ + H2O
-
 Micrococcus luteus ? + NADH
-
 ?
1.1.1.192 additional information the wild type enzyme shows no activity with NADP+ Micrococcus luteus ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.1.1.192 NAD+-dependent secondary alcohol dehydrogenase
-
 Micrococcus luteus
1.1.1.192 SADH
-
 Micrococcus luteus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.192 NAD+
-
 Micrococcus luteus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.192 0.00061
-
 12-hydroxyoctadecanoic acid wild type enzyme, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus
1.1.1.192 0.0014
-
 10-hydroxyoctadecanoic acid mutant enzyme D37S/V39S, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus
1.1.1.192 0.0014
-
 10-hydroxyoctadecanoic acid wild type enzyme, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus
1.1.1.192 0.0015
-
 12-hydroxyoctadecanoic acid mutant enzyme D37S/V39S, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus
1.1.1.192 0.0021
-
 10,12-dihydroxyoctadecanoic acid mutant enzyme D37S/A38R/V39S/T15I, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus
1.1.1.192 0.0035
-
 10,12-dihydroxyoctadecanoic acid wild type enzyme, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus
1.1.1.192 0.012
-
 10,12-dihydroxyoctadecanoic acid mutant enzyme D37S/V39S, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus
1.1.1.192 0.013
-
 10-hydroxyoctadecanoic acid mutant enzyme D37S/A38R/V39S/T15I, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus