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Literature summary extracted from

  • Zuhra, K.; Tome, C.S.; Masi, L.; Giardina, G.; Paulini, G.; Malagrino, F.; Forte, E.; Vicente, J.B.; Giuffre, A.
    N-acetylcysteine serves as substrate of 3-mercaptopyruvate sulfurtransferase and stimulates sulfide metabolism in colon cancer cells (2019), Cells, 8, 828 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.8.1.2 medicine colon cancer cells exposed to N-acetylcysteine for 24 h display increased expression and activity of MST and sulfide:quinone oxidoreductase. N-acetylcysteine persists at detectable levels inside the cells exposed to the drug for up to 24 h and sustains H2S synthesis by MST more effectively than cysteine Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.2 6
-
 L-cysteine pH 8.0, 37°C Homo sapiens
2.8.1.2 11.4
-
 N-acetyl-L-cysteine pH 8.0, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.1.2 mitochondrion partly Homo sapiens 5739
-

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.2 Homo sapiens P25325
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.1.2 SW-480 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.2 2-oxo-3-sulfanylpropanoate + [3-mercaptopyruvate sulfurtransferase]-L-cysteine
-
 Homo sapiens pyruvate + [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine
-
 ?
2.8.1.2 7-azido-4-methylcoumarin + L-cysteine
-
 Homo sapiens ?
-
 ?
2.8.1.2 7-azido-4-methylcoumarin + N-acetyl-L-cysteine
-
 Homo sapiens ?
-
 ?
2.8.1.2 [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine + reduced thioredoxin
-
 Homo sapiens hydrogen sulfide + [3-mercaptopyruvate sulfurtransferase]-L-cysteine + oxidized thioredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.1.2 MPST
-
 Homo sapiens
2.8.1.2 MST
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
2.8.1.2 Homo sapiens N-acetylcysteine, presence for 24 h, about 60% increase in expression up