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Literature summary extracted from

  • Boudet, J.; Devillier, J.C.; Wiegand, T.; Salmon, L.; Meier, B.H.; Lipps, G.; Allain, F.H.
    A small helical bundle prepares primer synthesis by binding two nucleotides that enhance sequence-specific recognition of the DNA template (2019), Cell, 176, 154-166.e13 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.102 ATP ATP binding enhances sequence-specific DNA recognition for primase anchoring Sulfolobus islandicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.102
-
 Sulfolobus islandicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.102 ssDNA + n dNTP Sulfolobus islandicus primases have a fundamental role in DNA replication. They synthesize a primer that is then extended by DNA polymerases ssDNA/pppdN(pdN)n-1 hybrid + (n-1) diphosphate
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Organism

EC Number Organism UniProt Comment Textmining
2.7.7.102 Sulfolobus islandicus Q54324
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-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.102
-
 Sulfolobus islandicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.102 ssDNA + n dNTP primases have a fundamental role in DNA replication. They synthesize a primer that is then extended by DNA polymerases Sulfolobus islandicus ssDNA/pppdN(pdN)n-1 hybrid + (n-1) diphosphate
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 ?
2.7.7.102 ssDNA + n dNTP a small helical bundle prepares primer synthesis by binding two nucleotides that enhance sequence-specific recognition of the DNA template. Archaeoeukaryotic primases require for synthesis a catalytic and an accessory domain. For the pRN1 archaeal primase, this domain is a 115-amino acid helix bundle domain (HBD). Only the HBD binds the DNA template. DNA binding becomes sequence-specific after a major allosteric change in the HBD, triggered by the binding of two nucleotide triphosphates Sulfolobus islandicus ssDNA/pppdN(pdN)n-1 hybrid + (n-1) diphosphate
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.102 primase HBD
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 Sulfolobus islandicus

General Information

EC Number General Information Comment Organism
2.7.7.102 physiological function primases have a fundamental role in DNA replication. They synthesize a primer that is then extended by DNA polymerases Sulfolobus islandicus