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Literature summary extracted from

  • Batten, L.; Parnell, A.; Wells, N.; Murch, A.; Oyston, P.; Roach, P.
    Biochemical and structural characterization of polyphosphate kinase 2 from the intracellular pathogen Francisella tularensis (2016), Biosci. Rep., 36, e00294 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.1 expression in Escherichia coli Francisella tularensis subsp. tularensis
2.7.4.34 expression in Escherichia coli Francisella tularensis subsp. tularensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.1 structure to 2.23 resolution. The structure consists of a six-stranded parallel beta-sheet surrounded by 12 alpha-helices, with a high degree of similarity to other members of the PPK2 family Francisella tularensis subsp. tularensis
2.7.4.34 structure to 2.23 resolution. The structure consists of a six-stranded parallel beta-sheet surrounded by 12 alpha-helices, with a high degree of similarity to other members of the PPK2 family Francisella tularensis subsp. tularensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.1 0.372
-
 ATP pH 8, 37°C Francisella tularensis subsp. tularensis
2.7.4.1 0.546
-
 ADP pH 8, 37°C Francisella tularensis subsp. tularensis
2.7.4.34 0.692
-
 GTP pH 8, 37°C Francisella tularensis subsp. tularensis
2.7.4.34 0.727
-
 GDP pH 8, 37°C Francisella tularensis subsp. tularensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Mg2+ optimum concentration 10 mM Francisella tularensis subsp. tularensis
2.7.4.1 Mn2+ optimum concentration 1 mM Francisella tularensis subsp. tularensis
2.7.4.34 Mg2+ optimum concentration 10 mM Francisella tularensis subsp. tularensis
2.7.4.34 Mn2+ optimum concentration 1 mM Francisella tularensis subsp. tularensis

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Francisella tularensis subsp. tularensis Q5NEQ5 cf. EC 2.7.4.34
-
2.7.4.34 Francisella tularensis subsp. tularensis Q5NEQ5 cf. EC 2.7.4.1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.1 0.25
-
 synthesis of ATP, pH 8, 37°C Francisella tularensis subsp. tularensis
2.7.4.34 0.244
-
 synthesis of GTP, pH 8, 37°C Francisella tularensis subsp. tularensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ADP + (phosphate)n+1
-
 Francisella tularensis subsp. tularensis ATP + (phosphate)n
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Francisella tularensis subsp. tularensis ADP + (phosphate)n+1
-
 r
2.7.4.1 additional information enzyme also accepts GDP, reaction of Ec 2.7.4.34. No substrates: CDP, UDP Francisella tularensis subsp. tularensis ?
-
-
2.7.4.34 ADP + (phosphate)n+1 reaction of EC 2.7.4.1 Francisella tularensis subsp. tularensis ATP + (phosphate)n
-
 r
2.7.4.34 GDP + (phosphate)n+1
-
 Francisella tularensis subsp. tularensis GTP + (phosphate)n
-
 r
2.7.4.34 GTP + (phosphate)n
-
 Francisella tularensis subsp. tularensis GDP + (phosphate)n+1
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 FTT_1564
-
 Francisella tularensis subsp. tularensis
2.7.4.34 FTT_1564
-
 Francisella tularensis subsp. tularensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.1 1.46
-
 ATP pH 8, 37°C Francisella tularensis subsp. tularensis
2.7.4.1 3.17
-
 ADP pH 8, 37°C Francisella tularensis subsp. tularensis
2.7.4.34 2.77
-
 GTP pH 8, 37°C Francisella tularensis subsp. tularensis
2.7.4.34 3.69
-
 GDP pH 8, 37°C Francisella tularensis subsp. tularensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.1 8
-
-
 Francisella tularensis subsp. tularensis
2.7.4.34 8
-
-
 Francisella tularensis subsp. tularensis

General Information

EC Number General Information Comment Organism
2.7.4.1 physiological function a PPK2 mutant strain shows significantly increased sensitivity to a range of antibiotics in a manner independent of the mode of action of the antibiotic Francisella tularensis subsp. tularensis
2.7.4.34 physiological function a PPK2 mutant strain shows significantly increased sensitivity to a range of antibiotics in a manner independent of the mode of action of the antibiotic Francisella tularensis subsp. tularensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.4.1 3.92
-
 ATP pH 8, 37°C Francisella tularensis subsp. tularensis
2.7.4.1 5.788
-
 ADP pH 8, 37°C Francisella tularensis subsp. tularensis
2.7.4.34 4.002
-
 GTP pH 8, 37°C Francisella tularensis subsp. tularensis
2.7.4.34 5.075
-
 GDP pH 8, 37°C Francisella tularensis subsp. tularensis