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Literature summary extracted from

  • Shimohira, T.; Kurogi, K.; Liu, M.C.; Suiko, M.; Sakakibara, Y.
    The critical role of His48 in mouse cytosolic sulfotransferase SULT2A8 for the 7alpha-hydroxyl sulfation of bile acids (2018), Biosci. Biotechnol. Biochem., 82, 1359-1365 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.14 gene Sult2a8, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia ocli strain BL21 Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.14 H48N site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Mus musculus
2.8.2.14 H48T site-directed mutagenesis, inactive mutant Mus musculus
2.8.2.14 L99H site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.14 0.0012
-
 cholic acid pH 6.3, 37°C, recombinant wild-type enzyme Mus musculus
2.8.2.14 0.1859
-
 cholic acid pH 6.3, 37°C, recombinant mutant H48N Mus musculus
2.8.2.14 0.3935
-
 cholic acid pH 6.3, 37°C, recombinant mutant L99H Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.14 cytosol
-
 Mus musculus 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.14 3'-phosphoadenylyl sulfate + chenodeoxycholic acid Mus musculus
-
 adenosine 3',5'-bisphosphate + chenodeoxycholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + cholic acid Mus musculus high activity adenosine 3',5'-bisphosphate + cholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + glycocholic acid Mus musculus high activity adenosine 3',5'-bisphosphate + glycocholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + taurochenodeoxycholic acid Mus musculus
-
 adenosine 3',5'-bisphosphate + taurochenodeoxycholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + taurocholic acid Mus musculus best substrate adenosine 3',5'-bisphosphate + taurocholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + ursodeoxycholic acid Mus musculus
-
 adenosine 3',5'-bisphosphate + ursodeoxycholate 3-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.14 Mus musculus Q8BGL3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.14 recombinant GST-tagged wild-type and mutant enzymes from Escherichia ocli strain BL21 by glutathione affinity chromatography, the tag is cleaved off by bovine thrombin Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.2.14 3'-phosphoadenylyl sulfate + glycolithocholate = adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate catalytic mechanism of SULT2A8 Mus musculus
a
2.8.2.14 3'-phosphoadenylyl sulfate + taurolithocholate = adenosine 3',5'-bisphosphate + taurolithocholate sulfate catalytic mechanism of SULT2A8 Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.14 6.31
-
 pH 6.3, 37°C, purified recombinant wild-type enzyme, substrate ursodeoxycholic acid Mus musculus
2.8.2.14 7.43
-
 pH 6.3, 37°C, purified recombinant enzyme mutant H48N, substrate cholic acid Mus musculus
2.8.2.14 8.72
-
 pH 6.3, 37°C, purified recombinant enzyme mutant H48N, substrate glycocholic acid Mus musculus
2.8.2.14 11.6
-
 pH 6.3, 37°C, purified recombinant enzyme mutant L99H, substrate glycocholic acid Mus musculus
2.8.2.14 26.3
-
 pH 6.3, 37°C, purified recombinant enzyme mutant L99H, substrate cholic acid Mus musculus
2.8.2.14 38.1
-
 pH 6.3, 37°C, purified recombinant wild-type enzyme, substrate chenodeoxycholic acid Mus musculus
2.8.2.14 70.7
-
 pH 6.3, 37°C, purified recombinant wild-type enzyme, substrate cholic acid Mus musculus
2.8.2.14 74.1
-
 pH 6.3, 37°C, purified recombinant wild-type enzyme, substrate taurochenodeoxycholic acid Mus musculus
2.8.2.14 89.1
-
 pH 6.3, 37°C, purified recombinant wild-type enzyme, substrate glycocholic acid Mus musculus
2.8.2.14 89.5
-
 pH 6.3, 37°C, purified recombinant wild-type enzyme, substrate taurocholic acid Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.14 3'-phosphoadenylyl sulfate + chenodeoxycholic acid
-
 Mus musculus adenosine 3',5'-bisphosphate + chenodeoxycholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + cholic acid high activity Mus musculus adenosine 3',5'-bisphosphate + cholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + glycocholic acid high activity Mus musculus adenosine 3',5'-bisphosphate + glycocholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + taurochenodeoxycholic acid
-
 Mus musculus adenosine 3',5'-bisphosphate + taurochenodeoxycholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + taurocholic acid best substrate Mus musculus adenosine 3',5'-bisphosphate + taurocholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + ursodeoxycholic acid
-
 Mus musculus adenosine 3',5'-bisphosphate + ursodeoxycholate 3-sulfate
-
 ?
2.8.2.14 additional information mouse SULT2A8 displays 7alpha-hydroxyl bile acid-preferring activity and is likely responsible for the production of bile acid-7-O-sulfates. SULT2A8 exhibits the highest substrate affinity and catalytic activity toward cholic acid among other 7alpha-hydroxyl bile acids. No activity with deoxycholic acid, pregnenolone, and dehydroepiandrosterone Mus musculus ?
-
-

Subunits

EC Number Subunits Comment Organism
2.8.2.14 ? x * 32295, sequence calculation, x * 33000, recombinant detagged enzyme, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.8.2.14 sulfotransferase
-
 Mus musculus
2.8.2.14 SULT2A8
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.14 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.14 6.3
-
 assay at Mus musculus

General Information

EC Number General Information Comment Organism
2.8.2.14 malfunction substitutions of the two amino acids His48 and Leu99, which might have occurred during the evolution of the mouse SULT2A8 gene, endow mouse SULT2A8 the capacity to catalyze the 7-O-sulfation of bile acids Mus musculus
2.8.2.14 additional information critical role of His48 in mouse cytosolic sulfotransferase SULT2A8 for the 7alpha-hydroxyl sulfation of bile acids. SULT2A8 lacks a conservative catalytic His residue at position 99th. Residues, His48 and Leu99 are unique to the mouse SULT2A8, but not other SULTs in general. They are essential for the enzyme's 7-O-sulfating activity toward bile acids and the strict substrate specificity toward 7alpha-hydroxyl bile acids Mus musculus
2.8.2.14 physiological function members of the cytosolic sulfotransferase (SULT) SULT2A subfamily are known to be critically involved in the homeostasis of steroids and bile acids. SULT2A8, a 7alpha-hydroxyl bile acid-preferring mouse SULT, has been identified as the major enzyme responsible for the mouse-specific 7-O-sulfation of bile acids Mus musculus