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Literature summary extracted from

  • Ano, Y.; Hours, R.; Akakabe, Y.; Kataoka, N.; Yakushi, T.; Matsushita, K.; Adachi, O.
    Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose (2017), Biosci. Biotechnol. Biochem., 81, 411-418 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.22 EDTA treatment of the membrane fraction causes a clear decrease in D-pentonate 4-dehydrogenase activity, activity is reactivated to almost the original level by the addition of pyrroloquinoline quinone and Ca2+ Gluconobacter thailandicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.22 9.7
-
 D-ribonate pH 5.0, 25°C Gluconobacter thailandicus
1.1.99.22 10.5
-
 D-arabonate pH 5.0, 25°C Gluconobacter thailandicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.22 membrane
-
 Gluconobacter thailandicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.99.22 Ca2+ EDTA-treated enzyme is reactivated to almost the original level by the addition of pyrroloquinoline quinone and Ca2+ Gluconobacter thailandicus
1.1.99.22 additional information metallic ions, Mg2+, Sr2+, Ba2+, and several kinds of lantanides, are ineffective in reactivation of EDTA-trreated enzyme Gluconobacter thailandicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.22 Gluconobacter thailandicus M9MJR9
-
-
1.1.99.22 Gluconobacter thailandicus NBRC 3255 M9MJR9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.22 n-octyl-beta-D-thioglucoside, n-octyl-beta-D-glucoside, Mydol 10, and Mydol 12 are effective detergents to solubilize the enzyme Gluconobacter thailandicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.22 D-arabitol + 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus D-xylulose + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.22 D-arabonate + 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus 4-dehydro-D-arabonate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.22 D-arabonate + 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus NBRC 3255 4-dehydro-D-arabonate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.22 D-erythronate + 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus 3-dehydro-D-erythronate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.22 D-erythronate + 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus NBRC 3255 3-dehydro-D-erythronate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.22 D-fructose + oxidized 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus 5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.22 D-fructose + oxidized 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus NBRC 3255 5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.22 D-gluconate + 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus 5-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.22 D-psicose + 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus 5-dehydro-D-psicose + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.22 D-psicose + 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus NBRC 3255 5-dehydro-D-psicose + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.22 D-ribitol + 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.22 D-ribitol + 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus NBRC 3255 ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.22 D-ribonate + 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus 4-dehydro-D-ribonate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.22 D-sorbitol + 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus L-sorbose + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.22 glycerol + 2,6-dichlorophenolindophenol
-
 Gluconobacter thailandicus dihydroxyacetone + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.22 ? x *14000, plus x * 80000, SDS-PAGE Gluconobacter thailandicus

Synonyms

EC Number Synonyms Comment Organism
1.1.99.22 D-sorbitol dehydrogenase subunit SldA
-
 Gluconobacter thailandicus
1.1.99.22 GLDH
-
 Gluconobacter thailandicus
1.1.99.22 NBRC3255_0235
-
 Gluconobacter thailandicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.22 4
-
-
 Gluconobacter thailandicus