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Literature summary extracted from

  • Su, H.; Peng, F.; Xu, P.; Wu, X.; Zong, M.; Yang, J.; Lou, W.
    Enhancing the thermostability and activity of uronate dehydrogenase from Agrobacterium tumefaciens LBA4404 by semi-rational engineering (2019), Bioresour. Bioprocess., 6, 36 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.203 expressed in Escherichia coli BL21(DE3) cells Agrobacterium tumefaciens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.203 A39P the mutant shows 103.5% activity compared to the wild type enzyme Agrobacterium tumefaciens
1.1.1.203 A39P/H234K the mutant shows 165.7% activity compared to the wild type enzyme Agrobacterium tumefaciens
1.1.1.203 A39P/H99Y the mutant shows 146.8% activity compared to the wild type enzyme Agrobacterium tumefaciens
1.1.1.203 A39P/H99Y/H234K the triple mutant shows 247.6% activity compared to the wild type enzyme, a 400fold improvement in half-life at 59°C, and a 5°C improvement in T50 (10 min) value Agrobacterium tumefaciens
1.1.1.203 H234K the mutant shows 129.4% activity compared to the wild type enzyme Agrobacterium tumefaciens
1.1.1.203 H99Y the mutant shows 109.6% activity compared to the wild type enzyme Agrobacterium tumefaciens
1.1.1.203 H99Y/H234K the mutant shows 175.4% activity compared to the wild type enzyme Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.203 0.15
-
 D-glucuronic acid mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 0.17
-
 D-glucuronic acid mutant enzyme H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 0.18
-
 D-glucuronic acid mutant enzyme A39P/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 0.2
-
 D-glucuronic acid wild type enzyme, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 0.2
-
 D-glucuronic acid mutant enzyme A39P/H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 0.23
-
 D-glucuronic acid mutant enzyme H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 0.24
-
 D-glucuronic acid mutant enzyme H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 0.26
-
 D-glucuronic acid mutant enzyme A39P, at pH 8.0 and 30°C Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.203 D-galacturonic acid + NAD+ + H2O Agrobacterium tumefaciens
-
 D-galactaric acid + NADH + H+
-
 ?
1.1.1.203 D-galacturonic acid + NAD+ + H2O Agrobacterium tumefaciens LBA4404
-
 D-galactaric acid + NADH + H+
-
 ?
1.1.1.203 D-glucuronic acid + NAD+ + H2O Agrobacterium tumefaciens
-
 D-glucaric acid + NADH + H+
-
 ?
1.1.1.203 D-glucuronic acid + NAD+ + H2O Agrobacterium tumefaciens LBA4404
-
 D-glucaric acid + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.203 Agrobacterium tumefaciens
-
-
-
1.1.1.203 Agrobacterium tumefaciens LBA4404
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.203 85
-
 wild type enzyme, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 88
-
 mutant enzyme A39P, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 93
-
 mutant enzyme H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 110
-
 mutant enzyme H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 124
-
 mutant enzyme A39P/H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 140
-
 mutant enzyme A39P/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 149
-
 mutant enzyme H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 210
-
 mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.203 D-galacturonic acid + NAD+ + H2O
-
 Agrobacterium tumefaciens D-galactaric acid + NADH + H+
-
 ?
1.1.1.203 D-galacturonic acid + NAD+ + H2O
-
 Agrobacterium tumefaciens LBA4404 D-galactaric acid + NADH + H+
-
 ?
1.1.1.203 D-glucuronic acid + NAD+ + H2O
-
 Agrobacterium tumefaciens D-glucaric acid + NADH + H+
-
 ?
1.1.1.203 D-glucuronic acid + NAD+ + H2O
-
 Agrobacterium tumefaciens LBA4404 D-glucaric acid + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.203 ? x * 32000, SDS-PAGE Agrobacterium tumefaciens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.203 Udh
-
 Agrobacterium tumefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.203 30
-
-
 Agrobacterium tumefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.203 59
-
 the enzyme has a half-life of 0.3 min at 59°C (pH 8.0) Agrobacterium tumefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.203 161
-
 D-glucuronic acid wild type enzyme, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 179
-
 D-glucuronic acid mutant enzyme H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 192
-
 D-glucuronic acid mutant enzyme A39P, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 195
-
 D-glucuronic acid mutant enzyme H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 239
-
 D-glucuronic acid mutant enzyme A39P/H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 295
-
 D-glucuronic acid mutant enzyme A39P/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 318
-
 D-glucuronic acid mutant enzyme H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 380
-
 D-glucuronic acid mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.203 8
-
-
 Agrobacterium tumefaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.203 NAD+
-
 Agrobacterium tumefaciens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.203 720
-
 D-glucuronic acid mutant enzyme A39P/H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 730
-
 D-glucuronic acid mutant enzyme A39P, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 770
-
 D-glucuronic acid mutant enzyme H99Y, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 800
-
 D-glucuronic acid wild type enzyme, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 810
-
 D-glucuronic acid mutant enzyme H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 840
-
 D-glucuronic acid mutant enzyme A39P/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 860
-
 D-glucuronic acid mutant enzyme H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens
1.1.1.203 910
-
 D-glucuronic acid mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C Agrobacterium tumefaciens