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Literature summary extracted from

  • Sachdeva, S.; Musayev, F.; Alhamadsheh, M.; Neel Scarsdale, J.; Tonie Wright, H.; Reynolds, K.
    Probing reactivity and substrate specificity of both subunits of the dimeric Mycobacterium tuberculosis FabH using alkyl-CoA disulfide inhibitors and acyl-CoA substrates (2008), Bioorg. Chem., 36, 85-90 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.301 expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.301 strucure of FabH with CoAdecyl disulfide inhibitor. The decyl chain binds in acyl-binding channels of both subunits through disulfide linkage to the active site cysteine. Both subunits of FabH can react with substrates or inhibitor Mycobacterium tuberculosis

Engineering

EC Number Protein Variants Comment Organism
2.3.1.301 C122A loss of activity Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.301 Mycobacterium tuberculosis P9WNG3
-
-
2.3.1.301 Mycobacterium tuberculosis ATCC 25618 P9WNG3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.301 octanoyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis 3-oxodecanoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.301 octanoyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis ATCC 25618 3-oxodecanoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.301 decanoyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis 3-oxolauroyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.301 decanoyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis ATCC 25618 3-oxolauroyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.301 lauroyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis 3-oxomyristoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.301 lauroyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis ATCC 25618 3-oxomyristoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.301 myristoyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis 3-oxopalmitoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.301 myristoyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis ATCC 25618 3-oxopalmitoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.301 palmitoyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis 3-oxostearoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.301 palmitoyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis ATCC 25618 3-oxostearoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.301 dodecanoyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis 3-oxotetradecanoyl-[acyl-carrier protein] + CoA + CO2
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.301 dimer 2 * 36903, ESI mass spectrometry and crystallization data Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.3.1.301 metabolism reaction occurs at both subunits and shows strict specificity for dodecyl-CoA Mycobacterium tuberculosis