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Literature summary extracted from

  • Sun, Y.; Machalz, D.; Wolber, G.; Parr, M.; Bureik, M.
    Functional expression of all human sulfotransferases in fission yeast, assay development, and structural models for isoforms SULT4A1 and SULT6B1 (2020), Biomolecules, 10, 1-17 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.8.2.1 analysis a modified variant of sulfotransferase assay employs permeabilized fission yeast cells (enzyme bags). A new and convenient SULT activity assay is based on the sulfation of a proluciferin compound, which is catalyzed by SULT1E1, SULT2A1, SULT4A1, and SULT6B1 Homo sapiens
2.8.2.1 synthesis generation of a complete set of recombinant fission yeast strains each expressing one of the 14 human SULT enzymes. The intracellular production of the cofactor 3'-phosphoadenosine 5'-phosphosulfate (PAPS) necessary for SULT activity is sufficiently high to support metabolite production Homo sapiens
2.8.2.2 analysis a modified variant of sulfotransferase assay employs permeabilized fission yeast cells (enzyme bags). A new and convenient SULT activity assay is based on the sulfation of a proluciferin compound, which is catalyzed by SULT1E1, SULT2A1, SULT4A1, and SULT6B1 Homo sapiens
2.8.2.2 synthesis generation of a complete set of recombinant fission yeast strains each expressing one of the 14 human SULT enzymes. The intracellular production of the cofactor 3'-phosphoadenosine 5'-phosphosulfate (PAPS) necessary for SULT activity is sufficiently high to support metabolite production Homo sapiens
2.8.2.4 analysis a modified variant of sulfotransferase assay employs permeabilized fission yeast cells (enzyme bags). A new and convenient SULT activity assay is based on the sulfation of a proluciferin compound, which is catalyzed by SULT1E1, SULT2A1, SULT4A1, and SULT6B1 Homo sapiens
2.8.2.4 synthesis generation of a complete set of recombinant fission yeast strains each expressing one of the 14 human SULT enzymes. The intracellular production of the cofactor 3'-phosphoadenosine 5'-phosphosulfate (PAPS) necessary for SULT activity is sufficiently high to support metabolite production Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.2.1 homology modeling in complex with 3'-phosphoadenylyl sulfate Homo sapiens
2.8.2.2 homology modeling in complex with 3'-phosphoadenylyl sulfate Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Homo sapiens Q9BR01 isoform SULT4A1
-
2.8.2.2 Homo sapiens Q6IMI4 isoform SULT6B1
-
2.8.2.4 Homo sapiens P49888 isoform SULT1E1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + 1-naphthol
-
 Homo sapiens adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + 6-hydroxy-4-methylbenzo[d]thiazole-2-carbonitrile i.e. proluciferin substrate UGT-Glo substrate A, GSA Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.2 3'-phosphoadenylyl sulfate + 6-hydroxy-4-methylbenzo[d]thiazole-2-carbonitrile i.e. proluciferin substrate UGT-Glo substrate A, GSA Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.4 3'-phosphoadenylyl sulfate + 6-hydroxy-4-methylbenzo[d]thiazole-2-carbonitrile i.e. proluciferin substrate UGT-Glo substrate A, GSA Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.1 SULT4A1
-
 Homo sapiens
2.8.2.2 SULT6B1
-
 Homo sapiens
2.8.2.4 SULT1E1
-
 Homo sapiens