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Literature summary extracted from

  • Taxon, E.S.; Halbers, L.P.; Parsons, S.M.
    Kinetics aspects of Gamma-hydroxybutyrate dehydrogenase (2020), Biochim. Biophys. Acta Proteins Proteom., 1868, 140376 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.61 nudix hydrolase at optimal pH 9.0, the GHBDH reaction is activated about 2fold by saturating purified nudix hydrolases, namely Bacillus methanolicus activator (ACT, UniProt: I3EA59) and Escherichia coli NudF (UniProt Q93K97) proteins. At physiological pH values of about 7.0, ACT activates by more than 3.5-fold Cupriavidus necator

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.61 homology modeling of structure Cupriavidus necator

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.61 D193A loss of more than 99% of activity compared to wild-type Cupriavidus necator
1.1.1.61 H197A loss of more than 99% of activity compared to wild-type Cupriavidus necator
1.1.1.61 H261A loss of more than 99% of activity compared to wild-type Cupriavidus necator
1.1.1.61 H280A loss of more than 99% of activity compared to wild-type Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.61 NADH product inhibition, mixed type inhibition Cupriavidus necator
1.1.1.61 succinate semialdehyde product inhibition, mixed type inhibition with respect to NAD+, competititve with respect to 4-hydroxybutanoate Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.61 0.045
-
 NAD+ pH 9.0, 23°C Cupriavidus necator
1.1.1.61 0.52
-
 4-hydroxybutanoate pH 9.0, 23°C Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.61 Cupriavidus necator Q59104
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.61 4-hydroxybutanoate + NAD+
-
 Cupriavidus necator succinate semialdehyde + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.61 gbd
-
 Cupriavidus necator
1.1.1.61 GHBDH
-
 Cupriavidus necator
1.1.1.61 NAD-dependent 4-hydroxybutyrate dehydrogenase
-
 Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.61 9
-
-
 Cupriavidus necator

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.61 0.0084
-
 succinate semialdehyde substrate 4-hydroxybutanoate, pH 9, 23°C Cupriavidus necator
1.1.1.61 0.0118
-
 succinate semialdehyde substrate NAD+, pH 9, 23°C Cupriavidus necator
1.1.1.61 0.0236
-
 NADH substrate NAD+, pH 9, 23°C Cupriavidus necator
1.1.1.61 0.0933
-
 NADH substrate 4-hydroxybutanoate, pH 9, 23°C Cupriavidus necator