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Literature summary extracted from

  • Hori, M.; Gokita, M.; Yasue, M.; Honda, T.; Kohama, T.; Mashimo, M.; Nakamura, H.; Murayama, T.
    Down-regulation of ceramide kinase via proteasome and lysosome pathways in PC12 cells by serum withdrawal its protection by nerve growth factor and role in exocytosis (2020), Biochim. Biophys. Acta, 1867, 118714 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.138 recombinant expression of HA-tagged enzyme CerK in PC-12 cells, A-549cells, and HEK-293 T cells, quantitative real-time PCR analysis of CerK mRNA Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.138 additional information knockdown of CerK is performed with shRNA to silence CerK (shCerK, target sequence GTT (TATCGAGTCAAGAAAT)). Establishment of a stable CerK-knockdown cell line (shCerK) and a HA-tagged CerK expressing cell line using a retroviral vector. Serum withdrawal causes ubiquitination of HA-tagged CerK protein and downregulates both HA-tagged CerK protein and ceramide 1-phosphate formation within 6 h, and these downregulations are abolished by co-treatments with NGF or proteasome inhibitors such as MG132 and clasto-lactacystin. Treatment with the proteasome inhibitors increases HA-tagged CerK in puncture structures, possibly endosomes and/or vesicles, in cells. Treatment with the lysosome inhibitors reduces serum withdrawal-induced downregulation of HA-tagged CerK protein but not ceramide 1-phosphate formation. When knockdown or overexpression of CerK is performed, Ca2+-induced release of [3H] noradrenaline is reduced or enhanced, respectively, but neurite extension is not modified. There is a positive correlation between noradrenaline release and formation of ceramide 1-phosphate and/or HA-tagged CerK levels in NGF- and clasto-lactacystin-treated cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.138 N-[2-(benzoylamino)-6-benzothiazolyl]-tricyclo[3.3.1.13,7] decane-1-carboxamide NVP-231, a potent inhibitor of CerK Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.138 neurite
-
 Homo sapiens
-
-
2.7.1.138 synaptic vesicle
-
 Homo sapiens 8021
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.138 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.138 ATP + ceramide Homo sapiens
-
 ADP + ceramide 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.138 Homo sapiens Q8TCT0
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.138 phosphoprotein activity of CerK is regulated by post-translational modifications including phosphorylation Homo sapiens
2.7.1.138 ubiquitination levels of CerK are downregulated by the ubiquitin/proteasome and lysosome pathways and the former pathway-sensitive pool of CerK is suggested to be linked with exocytosis in PC12 cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.138 brain
-
 Homo sapiens
-
2.7.1.138 additional information in nerve growth factor (NGF)-treated cells, HA-tagged CerK is mainly localized in punctuate structures, possibly endosomes and/or vesicles, in the cytoplasm in both the bottom and nucleus phases, and HA-tagged CerK exists in the extended neurites observed in the bottom phase Homo sapiens
-
2.7.1.138 neuron
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.138 ATP + ceramide
-
 Homo sapiens ADP + ceramide 1-phosphate
-
 ?
2.7.1.138 additional information activity of CerK in cells is measured by the formation of NBD-C1P from a substrate NBD-ceramide Homo sapiens ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.1.138 CERK
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.138 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.138 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.138 ATP
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.1.138 malfunction knockdown of CerK and overexpression of HA-tagged CerK down- and upregulated the formation of ceramide-1-phosphate (C1P), respectively. When knockdown or overexpression of CerK is performed, Ca2+-induced release of [3H] noradrenaline is reduced or enhanced, respectively, but neurite extension is not modified. A limited change in cellular sphingolipid levels by knockdown of CerK. The levels of ceramide, sphingomyelin, and monohexosylceramide, including their total levels and levels of their subspecies with irrespective of N-acyl chain lengths, and those of sphingosine, sphingomyelin 1-phosphate, and their dihydro-forms are not affected by the CerK knockdown in PC12 cells Homo sapiens
2.7.1.138 metabolism involvement of the lysosome pathway in CerK levels and ceramide 1-phosphate formation Homo sapiens
2.7.1.138 physiological function ceramide kinase (CerK) phosphorylates ceramide to ceramide-1-phosphate (C1P). The activity of CerK is regulated by post-translational modifications including phosphorylation, CerK has a role in neuronal functions Homo sapiens