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Literature summary extracted from

  • Miller, B.R.; Kung, Y.
    Structural features and domain movements controlling substrate binding and cofactor specificity in class II HMG-CoA reductase (2018), Biochemistry, 57, 654-662 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B38 expressed in Escherichia coli BL21(DE3) cells Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.B38 enzyme bound to 3-hydroxy-3-methylglutaryl-CoA, hanging drop vapor diffusion method, using 100 mM Tris pH 8.5, 100-250 mM lithium sulfate, and 15-25% (w/v) PEG 4000. Enzyme bound to NADPH, hanging drop vapor diffusion method, using 100 mM Tris pH 8.5, 100-250 mM lithium sulfate, and 30-40% (w/v) PEG 4000 Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B38 0.0289
-
 NADPH at pH 7.4 and 37°C Streptococcus pneumoniae
1.1.1.B38 0.153
-
 NADH at pH 7.4 and 37°C Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.B38 3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+ Streptococcus pneumoniae
-
 mevalonate + CoA + 2 NAD+
-
 ?
1.1.1.B38 3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ Streptococcus pneumoniae
-
 mevalonate + CoA + 2 NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B38 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.B38 Ni-NTA column chromatography and Superdex 200 gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B38 3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
 Streptococcus pneumoniae mevalonate + CoA + 2 NAD+
-
 ?
1.1.1.B38 3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 Streptococcus pneumoniae mevalonate + CoA + 2 NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.B38 homodimer x-ray crystallography Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B38 3-hydroxy-3-methylglutaryl coenzyme A reductase
-
 Streptococcus pneumoniae
1.1.1.B38 class II HMG-CoA reductase
-
 Streptococcus pneumoniae
1.1.1.B38 HGMR
-
 Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.B38 0.131
-
 NADH at pH 7.4 and 37°C Streptococcus pneumoniae
1.1.1.B38 6.85
-
 NADPH at pH 7.4 and 37°C Streptococcus pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B38 NADH
-
 Streptococcus pneumoniae
1.1.1.B38 NADPH kinetically preferred cofactor Streptococcus pneumoniae

General Information

EC Number General Information Comment Organism
1.1.1.B38 metabolism key enzyme in the mevalonate pathway Streptococcus pneumoniae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.B38 0.86
-
 NADH at pH 7.4 and 37°C Streptococcus pneumoniae
1.1.1.B38 240
-
 NADPH at pH 7.4 and 37°C Streptococcus pneumoniae