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Literature summary extracted from

  • Ragwan, E.R.; Arai, E.; Kung, Y.
    New Crystallographic snapshots of large domain movements in bacterial 3-hydroxy-3-methylglutaryl coenzyme A reductase (2018), Biochemistry, 57, 5715-5725 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.88 expressed in Escherichia coli BL21(DE3) cells Delftia acidovorans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.88 apoenzyme, sitting drop vapor diffusion method, using 100 mM Bis-Tris (pH 5.5), 600 mM lithium sulfate, and 18% (w/v) PEG 3350. Mevalonate-bound enzyme, sitting drop vapor diffusion method, using 100 mM Bis-Tris (pH 6.5), 600 mM lithium sulfate, and 19% (w/v) PEG 3350. NADH-bound enzyme, sitting drop vapor diffusion method, using 100 mM sodium citrate (pH 5.0), 500 mM ammonium sulfate, and 600 mM lithium sulfate Delftia acidovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.88 citrate competitive inhibition with respect to 3-hydroxy-3-methylglutaryl-CoA Delftia acidovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.88 0.00734
-
 3-hydroxy-3-methylglutaryl-CoA at pH 7.4, temperature not specified in the publication Delftia acidovorans
1.1.1.88 0.0285
-
 NADH at pH 7.4, temperature not specified in the publication Delftia acidovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.88 3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+ Delftia acidovorans
-
 (R)-mevalonate + CoA + 2 NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.88 Delftia acidovorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.88 Ni-NTA column chromatography Delftia acidovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.88 3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
 Delftia acidovorans (R)-mevalonate + CoA + 2 NAD+
-
 ?
1.1.1.88 3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ extremely low activity with NADPH Delftia acidovorans (R)-mevalonate + CoA + 2 NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.88 3-hydroxy-3-methylglutaryl coenzyme A reductase
-
 Delftia acidovorans
1.1.1.88 HMG-CoA reductase
-
 Delftia acidovorans
1.1.1.88 HMGR
-
 Delftia acidovorans
1.1.1.88 mvaA
-
 Delftia acidovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.88 16.6
-
 NADH at pH 7.4, temperature not specified in the publication Delftia acidovorans
1.1.1.88 22.3
-
 3-hydroxy-3-methylglutaryl-CoA at pH 7.4, temperature not specified in the publication Delftia acidovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.88 NADH NADH-specific enzyme Delftia acidovorans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.88 170
-
 citrate at pH 7.4, temperature not specified in the publication Delftia acidovorans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.88 582
-
 NADH at pH 7.4, temperature not specified in the publication Delftia acidovorans
1.1.1.88 3040
-
 3-hydroxy-3-methylglutaryl-CoA at pH 7.4, temperature not specified in the publication Delftia acidovorans