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Literature summary extracted from

  • Will, N.; Piserchio, A.; Snyder, I.; Ferguson, S.B.; Giles, D.H.; Dalby, K.N.; Ghose, R.
    Structure of the C-terminal helical repeat domain of eukaryotic elongation factor 2 kinase (2016), Biochemistry, 55, 5377-5386 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.20 Calmodulin
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.20 wild type and mutants Y712A/Y713A and E717A of the C-terminal enzyme fragment eEF-2K627-725 are expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.20 E717A the mutation in the C-terminal fragment of the enzyme reduces elongation factor 2 phosphorylation activity Homo sapiens
2.7.11.20 Y712A the mutation in the C-terminal fragment of the enzyme reduces elongation factor 2 phosphorylation activity Homo sapiens
2.7.11.20 Y712A/Y713A the mutations in the C-terminal fragment of the enzyme abolish elongation factor 2 phosphorylation activity Homo sapiens
2.7.11.20 Y713A the mutation in the C-terminal fragment of the enzyme reduces elongation factor 2 phosphorylation activity Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.20 Ca2+ activates Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.20 ATP + [eukaryotic elongation factor 2] Homo sapiens
-
 ADP + [eukaryotic elongation factor 2] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.20 Homo sapiens O00418
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.20 Ni-NTA column chromatography, and Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.20 ATP + [eukaryotic elongation factor 2]
-
 Homo sapiens ADP + [eukaryotic elongation factor 2] phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.20 EEF-2K
-
 Homo sapiens
2.7.11.20 eukaryotic elongation factor 2 kinase
-
 Homo sapiens