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Literature summary extracted from

  • Keating, T.; Marshall, C.; Walsh, C.
    Vibriobactin biosynthesis in Vibrio cholerae VibH is an amide synthase homologous to nonribosomal peptide synthetase condensation domains (2000), Biochemistry, 39, 15513-15521 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.71 expression in Escherichia coli Vibrio cholerae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.71 0.00046
-
 2,3-Dihydroxybenzoate pH 7.5, 30°C Vibrio cholerae
6.2.1.71 0.016
-
 salicylate pH 7.5, 30°C Vibrio cholerae

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.71 Vibrio cholerae Q9FDB2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.71 ATP + 2,3-dihydroxybenzoate + [aryl-carrier protein EntB]
-
 Vibrio cholerae AMP + diphosphate + 2,3-dihydroxybenzoyl-[aryl-carrier protein EntB]
-
 ?
6.2.1.71 ATP + salicylate + holo-[aryl-carrier protein EntB]
-
 Vibrio cholerae AMP + diphosphate + salicyl-[aryl-carrier protein EntB]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.71 2,3-dihydroxybenzoate-AMP ligase
-
 Vibrio cholerae
6.2.1.71 vibE
-
 Vibrio cholerae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.71 0.49
-
 salicylate pH 7.5, 30°C Vibrio cholerae
6.2.1.71 2.3
-
 2,3-Dihydroxybenzoate pH 7.5, 30°C Vibrio cholerae

General Information

EC Number General Information Comment Organism
6.2.1.71 physiological function during biosynthesis of Vibrio cholerae siderophore vibriobactin, VibE activates 2,3-dihydroxybenzoate as the acyl adenylate and then transfers it to the free thiol of the phosphopantetheine arm of VibB's aryl carrier domain Vibrio cholerae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.71 30
-
 salicylate pH 7.5, 30°C Vibrio cholerae
6.2.1.71 5000
-
 2,3-Dihydroxybenzoate pH 7.5, 30°C Vibrio cholerae