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Literature summary extracted from

  • Rozova, O.N.; Khmelenina, V.N.; Mustakhimov, I.I.; But, S.Y.; Trotsenko, Y.A.
    Properties of malic enzyme from the aerobic methanotroph Methylosinus trichosporium (2019), Biochemistry (Moscow), 84, 390-397 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.40 expressed in Escherichia coli BL21(DE3) cells Methylosinus trichosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.40 acetyl-CoA acetyl-CoA (0.1 mM) exerts the greatest inhibitory effect leading to a residual activity of 24% Methylosinus trichosporium
1.1.1.40 ATP ATP at 2 mM inhibits the enzyme by 50%, but the activity is completely recovered if Mg2+ concentration increases to 5 mM Methylosinus trichosporium
1.1.1.40 Cd2+ in the presence of Mn2+, Cd2+ ions almost completely inhibit the enzyme activity (5.9% residual activity) Methylosinus trichosporium
1.1.1.40 diphosphate diphosphate at 2 mM inhibits the enzyme by 50%, but the activity is completely recovered if Mg2+ concentration increases to 5 mM Methylosinus trichosporium
1.1.1.40 Hydroxypyruvate hydroxypyruvate at 1 mM inhibits the enzyme activity by 45% Methylosinus trichosporium
1.1.1.40 NaCl in the presence of 50 mM KCl, 50 mM NaCl inhibits the enzyme activity by 40% Methylosinus trichosporium
1.1.1.40 Sn2+ 1 mM Sn2+ ions reduce the enzyme activity by 31% Methylosinus trichosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.40 0.047
-
NADPH at pH 7.0 and 30°C Methylosinus trichosporium
1.1.1.40 0.064
-
NADP+ at pH 7.0 and 30°C Methylosinus trichosporium
1.1.1.40 2.7
-
(S)-malate at pH 7.0 and 30°C Methylosinus trichosporium
1.1.1.40 6
-
pyruvate at pH 7.0 and 30°C Methylosinus trichosporium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.40 K+ 100% activity in the presence of K+ and Mg2+ (1 mM each), 123.3% activity in the presence of K+ plus Mn2+ (1 mM each) Methylosinus trichosporium
1.1.1.40 Mg2+ 100% activity in the presence of K+ plus Mg2+ (1 mM each), 115.2% activity in the presence of K+ plus NH4+ (1 mM each) Methylosinus trichosporium
1.1.1.40 Mn2+ 123.3% activity in the presence of K+ plus Mn2+ (1 mM each) Methylosinus trichosporium
1.1.1.40 NH4+ 115.2% activity in the presence of K+ plus NH4+ (1 mM each) Methylosinus trichosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.40 480000
-
gel filtration Methylosinus trichosporium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.40 (S)-malate + NADP+ Methylosinus trichosporium the enzyme demonstrates 4.7fold higher activity in the direction of malate decarboxylation compared with pyruvate carboxylation pyruvate + CO2 + NADPH + H+
-
?
1.1.1.40 pyruvate + CO2 + NADPH + H+ Methylosinus trichosporium
-
(S)-malate + NADP+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.40 Methylosinus trichosporium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.40 Ni-NTA agarose column chromatography Methylosinus trichosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.40 (S)-malate + NADP+ the enzyme demonstrates 4.7fold higher activity in the direction of malate decarboxylation compared with pyruvate carboxylation Methylosinus trichosporium pyruvate + CO2 + NADPH + H+
-
?
1.1.1.40 additional information the enzyme does not use NAD+ and does not show activity of oxaloacetate decarboxylation. NaHCO3 does not serve as a CO2 donor for carboxylation of pyruvate Methylosinus trichosporium ?
-
-
1.1.1.40 pyruvate + CO2 + NADPH + H+
-
Methylosinus trichosporium (S)-malate + NADP+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.40 homohexamer 6 * 80000, SDS-PAGE Methylosinus trichosporium
1.1.1.40 homohexamer 6 * 81500, calculated from amino acid sequence Methylosinus trichosporium

Synonyms

EC Number Synonyms Comment Organism
1.1.1.40 NADP+-ME
-
Methylosinus trichosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.40 65
-
-
Methylosinus trichosporium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.40 20 70 the enzyme is active in wide ranges of temperatures (20-70°C) Methylosinus trichosporium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.40 30 60 the enzyme activity is not decreased after incubating the protein for 3 h at 30-40°C, but decreased by half after heating for 1 h at 50°C, and 80% activity is lost after 5 min heating at 60°C Methylosinus trichosporium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.40 0.067
-
pyruvate at pH 7.0 and 30°C Methylosinus trichosporium
1.1.1.40 0.3
-
(S)-malate at pH 7.0 and 30°C Methylosinus trichosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.40 7
-
-
Methylosinus trichosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.40 6 9 the enzyme is active in wide ranges of pH (pH 6.0-9.0) Methylosinus trichosporium

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.40 2
-
at pH 7.0 and 30°C Methylosinus trichosporium ATP
1.1.1.40 2
-
at pH 7.0 and 30°C Methylosinus trichosporium diphosphate

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.40 0.01
-
pyruvate at pH 7.0 and 30°C Methylosinus trichosporium
1.1.1.40 0.117
-
(S)-malate at pH 7.0 and 30°C Methylosinus trichosporium