EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.40 | expressed in Escherichia coli BL21(DE3) cells | Methylosinus trichosporium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | acetyl-CoA | acetyl-CoA (0.1 mM) exerts the greatest inhibitory effect leading to a residual activity of 24% | Methylosinus trichosporium | |
1.1.1.40 | ATP | ATP at 2 mM inhibits the enzyme by 50%, but the activity is completely recovered if Mg2+ concentration increases to 5 mM | Methylosinus trichosporium | |
1.1.1.40 | Cd2+ | in the presence of Mn2+, Cd2+ ions almost completely inhibit the enzyme activity (5.9% residual activity) | Methylosinus trichosporium | |
1.1.1.40 | diphosphate | diphosphate at 2 mM inhibits the enzyme by 50%, but the activity is completely recovered if Mg2+ concentration increases to 5 mM | Methylosinus trichosporium | |
1.1.1.40 | Hydroxypyruvate | hydroxypyruvate at 1 mM inhibits the enzyme activity by 45% | Methylosinus trichosporium | |
1.1.1.40 | NaCl | in the presence of 50 mM KCl, 50 mM NaCl inhibits the enzyme activity by 40% | Methylosinus trichosporium | |
1.1.1.40 | Sn2+ | 1 mM Sn2+ ions reduce the enzyme activity by 31% | Methylosinus trichosporium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.40 | 0.047 | - |
NADPH | at pH 7.0 and 30°C | Methylosinus trichosporium | |
1.1.1.40 | 0.064 | - |
NADP+ | at pH 7.0 and 30°C | Methylosinus trichosporium | |
1.1.1.40 | 2.7 | - |
(S)-malate | at pH 7.0 and 30°C | Methylosinus trichosporium | |
1.1.1.40 | 6 | - |
pyruvate | at pH 7.0 and 30°C | Methylosinus trichosporium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | K+ | 100% activity in the presence of K+ and Mg2+ (1 mM each), 123.3% activity in the presence of K+ plus Mn2+ (1 mM each) | Methylosinus trichosporium | |
1.1.1.40 | Mg2+ | 100% activity in the presence of K+ plus Mg2+ (1 mM each), 115.2% activity in the presence of K+ plus NH4+ (1 mM each) | Methylosinus trichosporium | |
1.1.1.40 | Mn2+ | 123.3% activity in the presence of K+ plus Mn2+ (1 mM each) | Methylosinus trichosporium | |
1.1.1.40 | NH4+ | 115.2% activity in the presence of K+ plus NH4+ (1 mM each) | Methylosinus trichosporium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | 480000 | - |
gel filtration | Methylosinus trichosporium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.40 | (S)-malate + NADP+ | Methylosinus trichosporium | the enzyme demonstrates 4.7fold higher activity in the direction of malate decarboxylation compared with pyruvate carboxylation | pyruvate + CO2 + NADPH + H+ | - |
? | |
1.1.1.40 | pyruvate + CO2 + NADPH + H+ | Methylosinus trichosporium | - |
(S)-malate + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.40 | Methylosinus trichosporium | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.40 | Ni-NTA agarose column chromatography | Methylosinus trichosporium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.40 | (S)-malate + NADP+ | the enzyme demonstrates 4.7fold higher activity in the direction of malate decarboxylation compared with pyruvate carboxylation | Methylosinus trichosporium | pyruvate + CO2 + NADPH + H+ | - |
? | |
1.1.1.40 | additional information | the enzyme does not use NAD+ and does not show activity of oxaloacetate decarboxylation. NaHCO3 does not serve as a CO2 donor for carboxylation of pyruvate | Methylosinus trichosporium | ? | - |
- |
|
1.1.1.40 | pyruvate + CO2 + NADPH + H+ | - |
Methylosinus trichosporium | (S)-malate + NADP+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.40 | homohexamer | 6 * 80000, SDS-PAGE | Methylosinus trichosporium |
1.1.1.40 | homohexamer | 6 * 81500, calculated from amino acid sequence | Methylosinus trichosporium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.40 | NADP+-ME | - |
Methylosinus trichosporium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | 65 | - |
- |
Methylosinus trichosporium |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | 20 | 70 | the enzyme is active in wide ranges of temperatures (20-70°C) | Methylosinus trichosporium |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | 30 | 60 | the enzyme activity is not decreased after incubating the protein for 3 h at 30-40°C, but decreased by half after heating for 1 h at 50°C, and 80% activity is lost after 5 min heating at 60°C | Methylosinus trichosporium |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.40 | 0.067 | - |
pyruvate | at pH 7.0 and 30°C | Methylosinus trichosporium | |
1.1.1.40 | 0.3 | - |
(S)-malate | at pH 7.0 and 30°C | Methylosinus trichosporium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | 7 | - |
- |
Methylosinus trichosporium |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | 6 | 9 | the enzyme is active in wide ranges of pH (pH 6.0-9.0) | Methylosinus trichosporium |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.1.1.40 | 2 | - |
at pH 7.0 and 30°C | Methylosinus trichosporium | ATP | |
1.1.1.40 | 2 | - |
at pH 7.0 and 30°C | Methylosinus trichosporium | diphosphate |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.40 | 0.01 | - |
pyruvate | at pH 7.0 and 30°C | Methylosinus trichosporium | |
1.1.1.40 | 0.117 | - |
(S)-malate | at pH 7.0 and 30°C | Methylosinus trichosporium |