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Literature summary extracted from

  • Whitfield, H.; White, G.; Sprigg, C.; Riley, A.M.; Potter, B.V.L.; Hemmings, A.M.; Brearley, C.A.
    An ATP-responsive metabolic cassette comprised of inositol tris/tetrakisphosphate kinase 1 (ITPK1) and inositol pentakisphosphate 2-kinase (IPK1) buffers diphosphosphoinositol phosphate levels (2020), Biochem. J., 477, 2621-2638 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.134 gene ITPK1, sequence comparisons, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain Rosetta 2(DE3)pLysS Arabidopsis thaliana
2.7.1.158 gene IPK1, recombinant expression Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.134 1.2
-
 ATP pH 7.5, 25°C, recombinant enzyme Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.134 Mg2+ required Arabidopsis thaliana
2.7.1.158 Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.134 ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate Arabidopsis thaliana
-
 ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.158 ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate Arabidopsis thaliana
-
 ADP + 1D-myo-inositol hexakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.134 Arabidopsis thaliana Q9SBA5
-
-
2.7.1.158 Arabidopsis thaliana Q93YN9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.134 recombinant C-terminally His-tagged enzyme from Escherichia coli strain Rosetta 2(DE3)pLysS by nickel affinity chromatography and gel filtration Arabidopsis thaliana
2.7.1.158 recombinant enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.134 ATP + 1D-myo-inositol 1,4,6-trisphosphate
-
 Arabidopsis thaliana ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 ?
2.7.1.134 ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
-
 Arabidopsis thaliana ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.134 ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate enzyme ITPK1 has robust enantiospecific hydroxykinase activity against Ins(3,4,5,6)P4 Arabidopsis thaliana ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.134 ATP + 1D-myo-inositol 3,4,6-trisphosphate
-
 Arabidopsis thaliana ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 ?
2.7.1.134 additional information substrate docking calculations, substrate specificity of Arabidopsis thaliana ITPK1, overview. Lack of Ins(1,4,5,6)P4/Ins(3,4,5,6) P4 product for Ins(1,4,6)P3 or Ins(3,4,6)P3 substrates. Consistent with the phosphorylation of the enantiomeric pair Ins(1,4,5,6)P4/Ins (3,4,5,6)P4, ITPK1 shows phosphorylation of Ins(1,4,6)P3 at the 3-position and Ins(3,4,6)P3 at the 1-position. ITPK1 has robust enantiospecific hydroxykinase activity against Ins(3,4,5,6)P4. With inositol tris/tetrakisphosphate kinase 1 (ITPK1, EC 2.7.1.134) and inositol pentakisphosphate 2-kinase (IPK1, EC 2.7.1.158) combined at a 1:20 ATP:ADP ratio, InsP6 is converted via Ins(1,3,4,5,6)P5 to Ins(3,4,5,6) P4 and/or Ins(1,4,5,6)P4 with concomitant generation of ATP Arabidopsis thaliana ?
-
-
2.7.1.158 ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 Arabidopsis thaliana ADP + 1D-myo-inositol hexakisphosphate
-
 ?
2.7.1.158 additional information with inositol tris/tetrakisphosphate kinase 1 (ITPK1, EC 2.7.1.134) and inositol pentakisphosphate 2-kinase (IPK1, EC 2.7.1.158) combined at a 1:20 ATP:ADP ratio, InsP6 is converted via Ins(1,3,4,5,6)P5 to Ins(3,4,5,6) P4 and/or Ins(1,4,5,6)P4 with concomitant generation of ATP Arabidopsis thaliana ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.1.134 At5g42810
-
 Arabidopsis thaliana
2.7.1.134 inositol tris/tetrakisphosphate kinase 1
-
 Arabidopsis thaliana
2.7.1.134 ITPK1
-
 Arabidopsis thaliana
2.7.1.158 At5g42810
-
 Arabidopsis thaliana
2.7.1.158 inositol pentakisphosphate 2-kinase
-
 Arabidopsis thaliana
2.7.1.158 Ipk1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.134 25
-
 assay at Arabidopsis thaliana
2.7.1.158 25
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.134 7.5
-
 assay at Arabidopsis thaliana
2.7.1.158 7.5
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.134 ATP
-
 Arabidopsis thaliana
2.7.1.158 ATP
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.7.1.134 evolution Arabidopsis thaliana ITPK1, belongs to a class of enzyme that has been shown to possess 1-, 5- and 6-hydroxykinase and (InsP6) 5-phosphokinase activities Arabidopsis thaliana
2.7.1.134 metabolism inositol tris/tetrakisphosphate kinase 1 (ITPK1, EC 2.7.1.134) and inositol pentakisphosphate 2-kinase (IPK1, EC 2.7.1.158) comprise a reversible metabolic cassette converting Ins(3,4,5,6)P4 into 5-InsP7 and back in a nucleotide-dependent manner. Ability of Arabidopsis inositol tris/tetrakisphosphate kinase 1 to discriminate between symmetric and enantiomeric substrates in the production of diverse symmetric and asymmetric myo-inositol phosphate and diphospho-myo-inositol phosphate (inositol pyrophosphate) products Arabidopsis thaliana
2.7.1.134 additional information substrate/product analysis using anion exchange chromatography on an acid- and hydroxide-stable quaternary amine functionalized latex, substrate docking calculations, resolvement and measurement of diphosphoinositol phosphates beside lower inositol phosphates without the use of radiolabel, method, overview. Modeling of the binding of the enantiomers Ins(1,4,5,6)P4 and Ins(3,4,5,6)P4 to ITPK1. Significant interaction of the 4-phosphate of Ins(1,4,5,6)P4 and of the 6-phosphate of the Ins(3,4,5,6)P4 in site F. The respective 1- and 3-phosphates of Ins(1,4,5,6)P4 and Ins(3,4,5,6)P4 make contacts in site C. These subsites are likely determinants of the reactivity of ITPK1 Arabidopsis thaliana
2.7.1.158 metabolism inositol tris/tetrakisphosphate kinase 1 (ITPK1, EC 2.7.1.134) and inositol pentakisphosphate 2-kinase (IPK1, EC 2.7.1.158) comprise a reversible metabolic cassette converting Ins(3,4,5,6)P4 into 5-InsP7 and back in a nucleotide-dependent manner. Ability of Arabidopsis inositol tris/tetrakisphosphate kinase 1 to discriminate between symmetric and enantiomeric substrates in the production of diverse symmetric and asymmetric myo-inositol phosphate and diphospho-myo-inositol phosphate (inositol diphosphate) products Arabidopsis thaliana