EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.87 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Thermococcus kodakarensis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.87 | His6-tagged recombinant apoenzyme, hanging drop vapor diffusion method, using 100 mM HEPES-NaOH (pH 7.5), 200 mM NaCl, and 10% (v/v) 2-propanol. Enzyme in complex with homoisocitrate and Mn2+, hanging drop vapor diffusion method, using 100 mM HEPES-NaOH (pH 7.5), 200 mM NaCl, and 10% (v/v) 2-propanol. Enzyme in complex with isocitrate and Mn2+, hanging drop vapor diffusion method, using 100 mM Tris-HCl (pH 7.5), 200 mM NaCl, and 30% (v/v) 2-methyl-2,4-pentanediol. Enzyme in complex with 3-isopropylmalate and Mn2+, hanging drop vapor diffusion method, using 100 mM HEPES-NaOH (pH 7.5), 200 mM NaCl, and 36% (v/v) 2-methyl-2,4-pentanediol | Thermococcus kodakarensis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.87 | I82N | the mutant shows decreased catalytic efficiency with homoisocitrate and isocitrate compared to the wild type enzyme and has no activity with 3-isopropylmalate | Thermococcus kodakarensis |
1.1.1.87 | L81P | the mutant shows decreased catalytic efficiency with homoisocitrate, isocitrate and 3-isopropylmalate compared to the wild type enzyme | Thermococcus kodakarensis |
1.1.1.87 | L83R | the mutant shows decreased catalytic efficiency with homoisocitrate and isocitrate compared to the wild type enzyme and has no activity with 3-isopropylmalate | Thermococcus kodakarensis |
1.1.1.87 | S80A | the mutant shows decreased catalytic efficiency with homoisocitrate, isocitrate and 3-isopropylmalate compared to the wild type enzyme | Thermococcus kodakarensis |
1.1.1.87 | T71V | the mutant shows decreased catalytic efficiency with homoisocitrate and isocitrate, as well as increased catalytic efficiency with 3-isopropylmalate compared to the wild type enzyme | Thermococcus kodakarensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.87 | 0.0073 | - |
homoisocitrate | wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.014 | - |
isocitrate | wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.036 | - |
NAD+ | wild type enzyme, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.037 | - |
homoisocitrate | mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.042 | - |
homoisocitrate | mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.05 | - |
3-isopropylmalate | mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.064 | - |
homoisocitrate | wild type enzyme, with NADP+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.1 | - |
NAD+ | wild type enzyme, with isocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.16 | - |
NAD+ | mutant enzyme L83R, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.17 | - |
homoisocitrate | mutant enzyme L83R, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.25 | - |
NAD+ | mutant enzyme T71V, with isocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.3 | - |
isocitrate | mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.31 | - |
3-isopropylmalate | wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.32 | - |
NAD+ | mutant enzyme L81P, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.46 | - |
homoisocitrate | mutant enzyme I82N, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.5 | - |
isocitrate | mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.58 | - |
isocitrate | mutant enzyme L83R, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.6 | - |
NAD+ | mutant enzyme L81P, with isocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.62 | - |
NAD+ | mutant enzyme L83R, with isocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.64 | - |
NAD+ | mutant enzyme I82N, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.68 | - |
3-isopropylmalate | mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.71 | - |
3-isopropylmalate | mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.76 | - |
NAD+ | mutant enzyme S80A, with isocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.92 | - |
NAD+ | mutant enzyme S80A, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.92 | - |
NAD+ | wild type enzyme, with D-malate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.94 | - |
homoisocitrate | mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 1 | - |
NAD+ | mutant enzyme T71V, with 3-isopropylmalate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 1.2 | - |
isocitrate | mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 1.2 | - |
NAD+ | mutant enzyme S80A, with 3-isopropylmalate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 1.3 | - |
NAD+ | mutant enzyme L81P, with 3-isopropylmalate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 1.5 | - |
NAD+ | mutant enzyme T71V, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 1.5 | - |
NAD+ | wild type enzyme, with 3-isopropylmalate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 1.8 | - |
NAD+ | mutant enzyme I82N, with isocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 2.2 | - |
isocitrate | mutant enzyme I82N, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 4.5 | - |
D-malate | wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 7.6 | - |
NADP+ | wild type enzyme, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.87 | Mg2+ | Mn2+ or Mg2+ is required for this activity, and stronger activity is observed with Mn2+ | Thermococcus kodakarensis | |
1.1.1.87 | Mn2+ | Mn2+ or Mg2+ is required for this activity, and stronger activity is observed with Mn2+ | Thermococcus kodakarensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.87 | 238300 | - |
gel filtration | Thermococcus kodakarensis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.87 | Thermococcus kodakarensis | - |
- |
- |
1.1.1.87 | Thermococcus kodakarensis KUW1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.87 | Ni-NTA His-Bind column chromatography | Thermococcus kodakarensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.87 | 3-isopropylmalate + NAD+ | the enzyme has low catalytic efficiency toward 3-isopropylmalate | Thermococcus kodakarensis | alpha-ketoisocaproate + NADH + H+ + CO2 | - |
? | |
1.1.1.87 | 3-isopropylmalate + NAD+ | the enzyme has low catalytic efficiency toward 3-isopropylmalate | Thermococcus kodakarensis KUW1 | alpha-ketoisocaproate + NADH + H+ + CO2 | - |
? | |
1.1.1.87 | D-malate + NAD+ | weak activity | Thermococcus kodakarensis | ? | - |
? | |
1.1.1.87 | D-malate + NAD+ | weak activity | Thermococcus kodakarensis KUW1 | ? | - |
? | |
1.1.1.87 | homoisocitrate + NAD+ | highest activity | Thermococcus kodakarensis | alpha-ketoadipate + NADH + H+ + CO2 | - |
r | |
1.1.1.87 | homoisocitrate + NAD+ | highest activity | Thermococcus kodakarensis KUW1 | alpha-ketoadipate + NADH + H+ + CO2 | - |
r | |
1.1.1.87 | homoisocitrate + NADP+ | - |
Thermococcus kodakarensis | alpha-ketoadipate + NADPH + H+ + CO2 | - |
r | |
1.1.1.87 | isocitrate + NADP+ | - |
Thermococcus kodakarensis | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
1.1.1.87 | isocitrate + NADP+ | - |
Thermococcus kodakarensis KUW1 | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
1.1.1.87 | additional information | no activity with L-tartrate | Thermococcus kodakarensis | ? | - |
- |
|
1.1.1.87 | additional information | no activity with L-tartrate | Thermococcus kodakarensis KUW1 | ? | - |
- |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.87 | homotetramer | x-ray crystallography | Thermococcus kodakarensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.87 | beta-decarboxylating dehydrogenase | - |
Thermococcus kodakarensis |
1.1.1.87 | HIc dehydrogenase | - |
Thermococcus kodakarensis |
1.1.1.87 | HICDH | - |
Thermococcus kodakarensis |
1.1.1.87 | TK0280 | - |
Thermococcus kodakarensis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.87 | 0.099 | - |
NAD+ | wild type enzyme, with D-malate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.099 | - |
D-malate | wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.22 | - |
isocitrate | mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.71 | - |
isocitrate | mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 1 | - |
isocitrate | mutant enzyme I82N, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 1.1 | - |
isocitrate | mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 1.5 | - |
3-isopropylmalate | mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 2.3 | - |
3-isopropylmalate | mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 4.8 | - |
homoisocitrate | mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 4.8 | - |
NAD+ | wild type enzyme, with 3-isopropylmalate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 4.8 | - |
3-isopropylmalate | wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 5 | - |
NAD+ | wild type enzyme, with isocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 5 | - |
isocitrate | wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 6.6 | - |
isocitrate | mutant enzyme L83R, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 6.9 | - |
homoisocitrate | mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 9.4 | - |
3-isopropylmalate | mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 12 | - |
NADP+ | wild type enzyme, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 12 | - |
homoisocitrate | wild type enzyme, with NADP+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 13 | - |
homoisocitrate | mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 17 | - |
NAD+ | wild type enzyme, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 17 | - |
homoisocitrate | wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 43 | - |
homoisocitrate | mutant enzyme L83R, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 45 | - |
homoisocitrate | mutant enzyme I82N, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.87 | NAD+ | the enzyme prefers NAD+ to NADP+ as the coenzyme | Thermococcus kodakarensis | |
1.1.1.87 | NADP+ | - |
Thermococcus kodakarensis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.87 | metabolism | the enzyme is involved in lysine biosynthesis | Thermococcus kodakarensis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.87 | 0.022 | - |
D-malate | wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.11 | - |
NAD+ | wild type enzyme, with D-malate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.44 | - |
isocitrate | mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.45 | - |
isocitrate | mutant enzyme I82N, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.56 | - |
NAD+ | mutant enzyme I82N, with isocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.88 | - |
NAD+ | mutant enzyme T71V, with isocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.92 | - |
isocitrate | mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 0.93 | - |
NAD+ | mutant enzyme S80A, with isocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 1.2 | - |
NAD+ | mutant enzyme L81P, with 3-isopropylmalate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 1.6 | - |
NADP+ | wild type enzyme, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 1.8 | - |
NAD+ | mutant enzyme L81P, with isocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 1.9 | - |
NAD+ | mutant enzyme S80A, with 3-isopropylmalate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 2.2 | - |
3-isopropylmalate | mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 2.4 | - |
isocitrate | mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 3.2 | - |
NAD+ | mutant enzyme T71V, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 3.2 | - |
NAD+ | wild type enzyme, with 3-isopropylmalate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 5.1 | - |
homoisocitrate | mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 7.5 | - |
NAD+ | mutant enzyme S80A, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 9.4 | - |
NAD+ | mutant enzyme T71V, with 3-isopropylmalate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 11 | - |
NAD+ | mutant enzyme L83R, with isocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 11 | - |
isocitrate | mutant enzyme L83R, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 13 | - |
3-isopropylmalate | mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 15 | - |
3-isopropylmalate | wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 41 | - |
NAD+ | mutant enzyme L81P, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 46 | - |
3-isopropylmalate | mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 50 | - |
NAD+ | wild type enzyme, with isocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 70 | - |
NAD+ | mutant enzyme I82N, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 98 | - |
homoisocitrate | mutant enzyme I82N, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 190 | - |
homoisocitrate | mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 190 | - |
homoisocitrate | wild type enzyme, with NADP+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 250 | - |
homoisocitrate | mutant enzyme L83R, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 270 | - |
NAD+ | mutant enzyme L83R, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 310 | - |
homoisocitrate | mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 360 | - |
isocitrate | wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 470 | - |
NAD+ | wild type enzyme, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis | |
1.1.1.87 | 2300 | - |
homoisocitrate | wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C | Thermococcus kodakarensis |