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Literature summary extracted from

  • Naeem, M.; Rehman, A.; Shen, B.; Ye, L.; Yu, H.
    Semi-rational engineering of carbonyl reductase YueD for efficient biosynthesis of halogenated alcohols with in situ cofactor regeneration (2018), Biochem. Eng. J., 137, 62-70 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.184 G135A the mutant shows decreased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis
1.1.1.184 G180A the mutant shows slightly increased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis
1.1.1.184 I190A the mutant shows decreased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis
1.1.1.184 M186A the mutant shows increased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis
1.1.1.184 Q187A the mutant shows increased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis
1.1.1.184 V181A the mutant shows strongly increased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis
1.1.1.184 W144A the mutant shows slightly increased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis
1.1.1.184 Y141A the mutant shows slightly increased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Bacillus subtilis
-
-
-
1.1.1.184 Bacillus subtilis WB600
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 1-(3-nitrosophenyl)ethan-1-one + NADPH + H+ 86% conversion after 24 h, 99% R-enantiomeric excess Bacillus subtilis 1-(3-nitrosophenyl)ethan-1-ol + NADP+
-
 ?
1.1.1.184 1-(3-nitrosophenyl)ethan-1-one + NADPH + H+ 86% conversion after 24 h, 99% R-enantiomeric excess Bacillus subtilis WB600 1-(3-nitrosophenyl)ethan-1-ol + NADP+
-
 ?
1.1.1.184 1-(4-chlorophenyl)ethan-1-one + NADPH + H+ 41% conversion after 24 h, 99% S-enantiomeric excess Bacillus subtilis 1-(4-chlorophenyl)ethan-1-ol + NADP+
-
 ?
1.1.1.184 1-(4-chlorophenyl)ethan-1-one + NADPH + H+ 41% conversion after 24 h, 99% S-enantiomeric excess Bacillus subtilis WB600 1-(4-chlorophenyl)ethan-1-ol + NADP+
-
 ?
1.1.1.184 1-(4-nitrosophenyl)ethan-1-one + NADPH + H+ 30% conversion after 24 h, 73% S-enantiomeric excess Bacillus subtilis 1-(4-nitrosophenyl)ethan-1-ol + NADP+
-
 ?
1.1.1.184 1-(4-nitrosophenyl)ethan-1-one + NADPH + H+ 30% conversion after 24 h, 73% S-enantiomeric excess Bacillus subtilis WB600 1-(4-nitrosophenyl)ethan-1-ol + NADP+
-
 ?
1.1.1.184 1-phenylethan-1-one + NADPH + H+ 75% conversion after 24 h, 99% S-enantiomeric excess Bacillus subtilis 1-phenylethan-1-ol + NADP+
-
 ?
1.1.1.184 1-phenylethan-1-one + NADPH + H+ 75% conversion after 24 h, 99% S-enantiomeric excess Bacillus subtilis WB600 1-phenylethan-1-ol + NADP+
-
 ?
1.1.1.184 2,2,2-trifluoro-1-phenylethan-1-one + NADPH + H+ 99.6% conversion after 24 h, 91.5% R-enantiomeric excess Bacillus subtilis 2,2,2-trifluoro-1-phenylethan-1-ol + NADP+
-
 ?
1.1.1.184 2,2,2-trifluoro-1-phenylethan-1-one + NADPH + H+ 99.6% conversion after 24 h, 91.5% R-enantiomeric excess Bacillus subtilis WB600 2,2,2-trifluoro-1-phenylethan-1-ol + NADP+
-
 ?
1.1.1.184 2-chloro-1-(3,4-dichlorophenyl)ethan-1-one + NADPH + H+ 41% conversion after 24 h, 99% R-enantiomeric excess Bacillus subtilis 2-chloro-1-(3,4-dichlorophenyl)ethan-1-ol + NADP+
-
 ?
1.1.1.184 3-bromoacetophenone + NADPH + H+ 61% conversion after 24 h Bacillus subtilis 1-(3-bromophenyl)ethanol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 ? x * 27000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 YueD
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.184 20 30 more than 60% activity between 20 and 30°C Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.184 6 8 more than 50% activity between pH 6.0 and 8.0. The activity decreases rapidly when pH is below 6.0 or above 8.0 Bacillus subtilis