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Literature summary extracted from

  • Dai, L.; Qin, L.; Hu, Y.; Huang, J.; Hu, Z.; Min, J.; Sun, Y.; Guo, R.
    Structural dissection of unnatural ginsenoside-biosynthetic UDP-glycosyltransferase Bs-YjiC from Bacillus subtilis for substrate promiscuity (2021), Biochem. Biophys. Res. Commun., 534, 73-78 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.384 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.384 free enzyme and its binary complex with UDP, sitting drop vapor diffusion method, using 1.3 M Tri-sodium citrate dehydrate, and 0.1 M Bis-Tris propane (pH 7.0) Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.384 Bacillus subtilis O34539
-
-
2.4.1.384 Bacillus subtilis 168 O34539
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.384 Ni-NTA column chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.384 UDP-alpha-D-glucose + ginsenoside Rh2
-
 Bacillus subtilis ginsenoside F12 + UDP
-
 ?
2.4.1.384 UDP-alpha-D-glucose + ginsenoside Rh2
-
 Bacillus subtilis 168 ginsenoside F12 + UDP
-
 ?
2.4.1.384 UDP-alpha-D-glucose + protopanaxadiol
-
 Bacillus subtilis ginsenoside Rh2 + UDP
-
 ?
2.4.1.384 UDP-alpha-D-glucose + protopanaxadiol
-
 Bacillus subtilis 168 ginsenoside Rh2 + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.384 monomer 1 * 46000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.384 UDP-glycosyltransferase
-
 Bacillus subtilis
2.4.1.384 UGT
-
 Bacillus subtilis
2.4.1.384 YjiC
-
 Bacillus subtilis