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Literature summary extracted from

  • Kajiura, H.; Nakamura, Y.; Nishimura, M.; Ohashi, T.; Misaki, R.; Fujiyama, K.
    Characterization of Bombyx mori N-acetylglucosaminyltransferase II splicing variants (2020), Biochem. Biophys. Res. Commun., 529, 404-410 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.143 expressed in Sf9 insect cells Bombyx mori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.143 Golgi apparatus
-
 Bombyx mori 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.143 Mn2+ dependent on Bombyx mori

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.143 Bombyx mori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.143 Co2+ affinity column chromatography Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.143 larva
-
 Bombyx mori
-
2.4.1.143 silk gland
-
 Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.143 UDP-GlcNAc + GlcNAcMan3GlcNAc2-pyridylamine
-
 Bombyx mori UDP + GlcNAc2Man3GlcNAc2-pyridylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.143 ? x * about 70000, SDS-PAGE Bombyx mori

Synonyms

EC Number Synonyms Comment Organism
2.4.1.143 GnTII
-
 Bombyx mori
2.4.1.143 N-acetylglucosaminyltransferase II
-
 Bombyx mori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.143 30 40
-
 Bombyx mori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.143 5 50 splice variants GNTII-A, -B, -C, and -D show 70.3%, 81.9%, 44.4% and 91.9% relative activity after 1 h at 30, 50, 40 and 50°C, respectively Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.143 6.5 7
-
 Bombyx mori