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Literature summary extracted from

  • Bachochin, M.J.; Van Allen, M.; Barber, R.D.
    Characterization of a Rhodobacter sphaeroides primary fatty acid kinase (2021), Arch. Microbiol., 203, 861-864 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.1 expressed in Escherichia coli BL21(DE3) Rosetta-2 cells Cereibacter sphaeroides
2.7.2.18 expressed in Escherichia coli BL21(DE3) Rosetta-2 cells Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 3.1
-
 ATP with acetate as cosubstrate, at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 14.2
-
 valerate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 24.5
-
 Butyrate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 25.8
-
 propionate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 53.9
-
 acetate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 109
-
 Isobutyrate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 3.1
-
 ATP with acetate as cosubstrate, at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 14.2
-
 valerate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 24.5
-
 Butyrate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 25.8
-
 propionate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 53.9
-
 acetate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 109
-
 Isobutyrate at pH 5.7 and 37°C Cereibacter sphaeroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Mn2+ 10 mM used in assay conditions. The enzyme exhibits about 25% higher specific activity with manganese as a cofactor compared to magnesium Cereibacter sphaeroides
2.7.2.18 Mn2+ 10 mM used in assay conditions. The enzyme exhibits about 25% higher specific activity with manganese as a cofactor compared to magnesium Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Cereibacter sphaeroides
-
-
-
2.7.2.18 Cereibacter sphaeroides Q3IYF1
-
-
2.7.2.18 Cereibacter sphaeroides ATH 2.4.1 Q3IYF1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1 HisTrap column chromatography Cereibacter sphaeroides
2.7.2.18 HisTrap column chromatography Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate 100% activity Cereibacter sphaeroides ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + butyrate 59.5% activity compared to acetate Cereibacter sphaeroides ADP + butyryl phosphate
-
 r
2.7.2.1 ATP + heptanoate 22% activity compared to acetate Cereibacter sphaeroides ADP + heptyl phosphate
-
 r
2.7.2.1 ATP + hexanoate 38% activity compared to acetate Cereibacter sphaeroides ADP + hexyl phosphate
-
 r
2.7.2.1 ATP + isobutyrate 26.7% activity compared to acetate Cereibacter sphaeroides ADP + isobutyryl phosphate
-
 r
2.7.2.1 ATP + octanoate 8.4% activity compared to acetate Cereibacter sphaeroides ADP + octyl phosphate
-
 r
2.7.2.1 ATP + propionate 93.3% activity compared to acetate Cereibacter sphaeroides ADP + propionyl phosphate
-
 r
2.7.2.1 ATP + valerate 43.3% activity compared to acetate Cereibacter sphaeroides ADP + valeryl phosphate
-
 r
2.7.2.18 ATP + acetate 100% activity Cereibacter sphaeroides ADP + acetyl phosphate
-
 ?
2.7.2.18 ATP + acetate 100% activity Cereibacter sphaeroides ATH 2.4.1 ADP + acetyl phosphate
-
 ?
2.7.2.18 ATP + butyrate 59.5% activity compared to acetate Cereibacter sphaeroides ADP + butyryl phosphate
-
 ?
2.7.2.18 ATP + butyrate 59.5% activity compared to acetate Cereibacter sphaeroides ATH 2.4.1 ADP + butyryl phosphate
-
 ?
2.7.2.18 ATP + heptanoate 22% activity compared to acetate Cereibacter sphaeroides ADP + heptanoyl phosphate
-
 ?
2.7.2.18 ATP + hexanoate 38% activity compared to acetate Cereibacter sphaeroides ADP + hexanoyl phosphate
-
 ?
2.7.2.18 ATP + isobutyrate 26.7% activity compared to acetate Cereibacter sphaeroides ADP + isobutyryl phosphate
-
 ?
2.7.2.18 ATP + isobutyrate 26.7% activity compared to acetate Cereibacter sphaeroides ATH 2.4.1 ADP + isobutyryl phosphate
-
 ?
2.7.2.18 ATP + octanoate 8.4% activity compared to acetate Cereibacter sphaeroides ADP + octanoyl phosphate
-
 ?
2.7.2.18 ATP + propionate 93.3% activity compared to acetate Cereibacter sphaeroides ADP + propionyl phosphate
-
 ?
2.7.2.18 ATP + propionate 93.3% activity compared to acetate Cereibacter sphaeroides ATH 2.4.1 ADP + propionyl phosphate
-
 ?
2.7.2.18 ATP + valerate 43.3% activity compared to acetate Cereibacter sphaeroides ADP + valeryl phosphate
-
 ?
2.7.2.18 additional information the enzyme shows broad substrate specificity for primary fatty acids of varying lengths (C2-C8) Cereibacter sphaeroides ?
-
-
2.7.2.18 additional information the enzyme shows broad substrate specificity for primary fatty acids of varying lengths (C2-C8) Cereibacter sphaeroides ATH 2.4.1 ?
-
-

Subunits

EC Number Subunits Comment Organism
2.7.2.1 ? x * 47000, SDS-PAGE Cereibacter sphaeroides
2.7.2.18 ? x * 47000, SDS-PAGE Cereibacter sphaeroides

Synonyms

EC Number Synonyms Comment Organism
2.7.2.1 ACK
-
 Cereibacter sphaeroides
2.7.2.1 ackA
-
 Cereibacter sphaeroides
2.7.2.1 short chain fatty acid kinase
-
 Cereibacter sphaeroides
2.7.2.18 acetate kinase
-
 Cereibacter sphaeroides
2.7.2.18 ACK
-
 Cereibacter sphaeroides
2.7.2.18 short chain fatty acid kinase
-
 Cereibacter sphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.1 51
-
 the activity declines sharply at temperatures above 51°C, with 44% and 12% of activity remaining at 60°C and 67°C, respectively Cereibacter sphaeroides
2.7.2.18 51
-
 activity declines sharply at temperatures above 51°C, with 44% and 12% of activity remaining at 60°C and 67°C, respectively Cereibacter sphaeroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.1 65.4
-
 Isobutyrate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 80.2
-
 valerate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 118
-
 Butyrate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 172
-
 propionate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 177
-
 acetate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 250
-
 ATP with acetate as cosubstrate, at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 65.4
-
 Isobutyrate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 80.2
-
 valerate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 118
-
 Butyrate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 172
-
 propionate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 177
-
 acetate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 250
-
 ATP with acetate as cosubstrate, at pH 5.7 and 37°C Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 5.7
-
-
 Cereibacter sphaeroides
2.7.2.18 5.7
-
-
 Cereibacter sphaeroides

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.2.1 0.6
-
 Isobutyrate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 3.3
-
 acetate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 4.8
-
 Butyrate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 5.6
-
 valerate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 6.7
-
 propionate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.1 80.5
-
 ATP with acetate as cosubstrate, at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 0.6
-
 Isobutyrate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 3.3
-
 acetate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 4.8
-
 Butyrate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 5.6
-
 valerate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 6.7
-
 propionate at pH 5.7 and 37°C Cereibacter sphaeroides
2.7.2.18 80.5
-
 ATP with acetate as cosubstrate, at pH 5.7 and 37°C Cereibacter sphaeroides