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Literature summary extracted from

  • Beckmann, J.; Chodavarapu, S.; Doyle, B.
    Evaluation of a conserved tryptophanyl residue in donor substrate binding and catalysis by a phenol sulfotransferase (SULT1A1) (2020), Arch. Biochem. Biophys., 695, 108621 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.2.1 homology modeling of the active site. Two phenylalanyl and one tyrosinyl group help create a hydrophobic pocket for phenolic acceptor substrates. Two arginyl and one lysyl residues, plus other groups, aid in the binding of 3'-phosphoadenosine-5'-phospho(sulfate) via electrostatic forces and H-bonding. The conserved histidine serves as the general base to initiate phenoxide anion stabilization and attack on the 3'-phosphoadenosine-5'-phosphosulfate sulfuryl group Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.1 W53A mutation decreases thermal stabilizations by 3'-phosphoadenosine-5'-phosphate plus pentachlorophenol Bos taurus
2.8.2.1 W53L mutation decreases thermal stabilizations by 3'-phosphoadenosine-5'-phosphate plus pentachlorophenol Bos taurus
2.8.2.1 W53Y mutation decreases thermal stabilizations by 3'-phosphoadenosine-5'-phosphate plus pentachlorophenol Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.1 0.000526
-
 7-hydroxycoumarin wild-type, pH 7.4, temperature not specified in the publication Bos taurus
2.8.2.1 0.000815
-
 3'-phosphoadenylyl sulfate wild-type, pH 7.4, temperature not specified in the publication Bos taurus
2.8.2.1 0.001394
-
 3'-phosphoadenylyl sulfate mutant W53Y, pH 7.4, temperature not specified in the publication Bos taurus
2.8.2.1 0.00187
-
 7-hydroxycoumarin mutant W53A, pH 7.4, temperature not specified in the publication Bos taurus
2.8.2.1 0.00208
-
 7-hydroxycoumarin mutant W53Y, pH 7.4, temperature not specified in the publication Bos taurus
2.8.2.1 0.003
-
 7-hydroxycoumarin mutant W53L, pH 7.4, temperature not specified in the publication Bos taurus
2.8.2.1 0.00519
-
 3'-phosphoadenylyl sulfate mutant W53L, pH 7.4, temperature not specified in the publication Bos taurus
2.8.2.1 0.00524
-
 3'-phosphoadenylyl sulfate mutant W53A, pH 7.4, temperature not specified in the publication Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Bos taurus P50227 isoform SULT1A1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + 7-hydroxycoumarin
-
 Bos taurus adenosine 3',5'-bisphosphate + 7-hydroxycoumarin sulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.1 SULT1A1
-
 Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.2.1 42 45 30 min, 50% inactivation, wild-type and mutants Bos taurus
2.8.2.1 45
-
 melting temperature, wild-type peaks at 45°C, with a domain-suggestive shoulder at 49°C Bos taurus
2.8.2.1 60
-
 melting temperature, wild-type in presence of 3'-phosphoadenosine-5'-phosphate plus pentachlorophenol Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.2.1 0.069
-
 3'-phosphoadenylyl sulfate mutant W53A, pH 7.4, temperature not specified in the publication Bos taurus
2.8.2.1 0.125
-
 3'-phosphoadenylyl sulfate mutant W53L, pH 7.4, temperature not specified in the publication Bos taurus
2.8.2.1 0.141
-
 3'-phosphoadenylyl sulfate wild-type, pH 7.4, temperature not specified in the publication Bos taurus
2.8.2.1 0.18
-
 3'-phosphoadenylyl sulfate mutant W53Y, pH 7.4, temperature not specified in the publication Bos taurus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8.2.1 13
-
 3'-phosphoadenylyl sulfate mutant W53A, pH 7.4, temperature not specified in the publication Bos taurus
2.8.2.1 24
-
 3'-phosphoadenylyl sulfate mutant W53L, pH 7.4, temperature not specified in the publication Bos taurus
2.8.2.1 129
-
 3'-phosphoadenylyl sulfate mutant W53Y, pH 7.4, temperature not specified in the publication Bos taurus
2.8.2.1 173
-
 3'-phosphoadenylyl sulfate wild-type, pH 7.4, temperature not specified in the publication Bos taurus