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Literature summary extracted from

  • Hofer, M.; Diener, J.; Begander, B.; Kourist, R.; Sieber, V.
    Engineering of a borneol dehydrogenase from P. putida for the enzymatic resolution of camphor (2021), Appl. Microbiol. Biotechnol., 105, 3159-3167 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.198 expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp. ATCC 17453

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.198 E145G the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 E145L/Y188A the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 E145M/Y188A the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 E145M/Y188T the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 E145S/Y188A the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 E145S/Y188T the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 S145H the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 S145L the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 S145M the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 S145S the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 S146A the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 S146A/Y188A the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 S146A/Y188T the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 Y188A mutant catalyzes stereoselective reduction of racemic camphor with 23% conversion and 99% enantiomeric excess Pseudomonas putida
1.1.1.198 Y188A the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 Y188I the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 Y188L the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 Y188T mutant catalyzes stereoselective reduction of racemic camphor with 22% conversion and 99% enantiomeric excess Pseudomonas putida
1.1.1.198 Y188T the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.198 Y188V the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
1.1.1.227 Y188A mutant catalyzes stereoselective reduction of racemic camphor with 23% conversion and 99% enantiomeric excess Pseudomonas putida
1.1.1.227 Y188T mutant catalyzes stereoselective reduction of racemic camphor with 22% conversion and 99% enantiomeric excess Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.198 0.006
-
NAD+ wild type enzyme, at pH 7.5 and 30°C Pseudomonas sp. ATCC 17453
1.1.1.198 0.06
-
NAD+ wild-type, pH 7.5, 30°C Pseudomonas putida
1.1.1.198 0.095
-
(+)-borneol wild-type, pH 7.5, 30°C Pseudomonas putida
1.1.1.198 0.095
-
(+)-borneol wild type enzyme, at pH 7.5 and 30°C Pseudomonas sp. ATCC 17453
1.1.1.198 0.115
-
(-)-borneol wild type enzyme, at pH 7.5 and 30°C Pseudomonas sp. ATCC 17453
1.1.1.198 0.28
-
(+)-borneol mutant enzyme S146A/Y188A, at pH 7.5 and 30°C Pseudomonas sp. ATCC 17453
1.1.1.198 0.28
-
(+)-borneol mutant Y188A, pH 7.5, 30°C Pseudomonas putida
1.1.1.198 0.34
-
(-)-borneol mutant enzyme S146A/Y188A, at pH 7.5 and 30°C Pseudomonas sp. ATCC 17453
1.1.1.198 0.46
-
(+)-borneol mutant enzyme S146A/Y188T, at pH 7.5 and 30°C Pseudomonas sp. ATCC 17453
1.1.1.198 0.46
-
(+)-borneol mutant Y188T, pH 7.5, 30°C Pseudomonas putida
1.1.1.198 0.73
-
(-)-borneol mutant enzyme S146A/Y188T, at pH 7.5 and 30°C Pseudomonas sp. ATCC 17453
1.1.1.227 0.06
-
NAD+ wild-type, pH 7.5, 30°C Pseudomonas putida
1.1.1.227 0.11
-
(-)-borneol wild-type, pH 7.5, 30°C Pseudomonas putida
1.1.1.227 0.34
-
(-)-borneol mutant Y188A, pH 7.5, 30°C Pseudomonas putida
1.1.1.227 0.73
-
(-)-borneol mutant Y188T, pH 7.5, 30°C Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.198 (+)-borneol + NAD+ Pseudomonas sp. ATCC 17453
-
(+)-camphor + NADH + H+
-
r
1.1.1.198 (+)-camphor + NADH + H+ Pseudomonas sp. ATCC 17453
-
(+)-borneol + NAD+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.198 Pseudomonas putida
-
-
-
1.1.1.198 Pseudomonas putida ATCC 17453
-
-
-
1.1.1.198 Pseudomonas sp. ATCC 17453
-
-
-
1.1.1.227 Pseudomonas putida
-
-
-
1.1.1.227 Pseudomonas putida ATCC 17453
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.198 HisTrap column chromatography Pseudomonas sp. ATCC 17453

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.198 (+)-borneol + NAD+
-
Pseudomonas putida (+)-camphor + NADH + H+
-
r
1.1.1.198 (+)-borneol + NAD+
-
Pseudomonas sp. ATCC 17453 (+)-camphor + NADH + H+
-
r
1.1.1.198 (+)-borneol + NAD+
-
Pseudomonas putida ATCC 17453 (+)-camphor + NADH + H+
-
r
1.1.1.198 (+)-camphor + NADH + H+
-
Pseudomonas putida (+)-borneol + NAD+
-
r
1.1.1.198 (+)-camphor + NADH + H+
-
Pseudomonas sp. ATCC 17453 (+)-borneol + NAD+
-
r
1.1.1.198 (+)-camphor + NADH + H+
-
Pseudomonas putida ATCC 17453 (+)-borneol + NAD+
-
r
1.1.1.198 (+)-isoborneol + NAD+
-
Pseudomonas putida (+)-isocamphor + NADH + H+
-
?
1.1.1.198 (+)-isoborneol + NAD+
-
Pseudomonas putida ATCC 17453 (+)-isocamphor + NADH + H+
-
?
1.1.1.198 (+/-)-isoborneol + NADH + H+
-
Pseudomonas sp. ATCC 17453 ?
-
?
1.1.1.198 (-)-borneol + NAD+
-
Pseudomonas sp. ATCC 17453 (-)-camphor + NADH + H+
-
?
1.1.1.198 (1R)-(+)-camphor + (1S)-(-)-camphor + NADH + H+
-
Pseudomonas sp. ATCC 17453 (+)-borneol + (1S)-(-)-camphor + NAD+
-
?
1.1.1.198 additional information enzyme also catalyzes the reaction of (-)-borneol and (-)-camphor, reaction of EC 1.1.1.227. No substrates: isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, 2-butanol, L-carveol, and DL-menthol Pseudomonas putida ?
-
-
1.1.1.198 additional information isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, (+/-)-2-butanol, L-carveol, and DL-menthol are not accepted as substrates Pseudomonas sp. ATCC 17453 ?
-
-
1.1.1.198 additional information enzyme also catalyzes the reaction of (-)-borneol and (-)-camphor, reaction of EC 1.1.1.227. No substrates: isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, 2-butanol, L-carveol, and DL-menthol Pseudomonas putida ATCC 17453 ?
-
-
1.1.1.227 (-)-borneol + NAD+
-
Pseudomonas putida (-)-camphor + NADH + H+
-
r
1.1.1.227 (-)-camphor + NADH + H+
-
Pseudomonas putida (-)-borneol + NAD+
-
r
1.1.1.227 additional information additional substrates are (+)-borneol, (+)-camphor and (+)-isoborneol, reaction of EC 1.1.1.198. No substrates: isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, 2-butanol, L-carveol, and DL-menthol Pseudomonas putida ?
-
-
1.1.1.227 additional information additional substrates are (+)-borneol, (+)-camphor and (+)-isoborneol, reaction of EC 1.1.1.198. No substrates: isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, 2-butanol, L-carveol, and DL-menthol Pseudomonas putida ATCC 17453 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.1.1.198 ? x * 31000, SDS-PAGE Pseudomonas sp. ATCC 17453
1.1.1.198 ? x * 27500, calculated from amino acid sequence Pseudomonas sp. ATCC 17453
1.1.1.198 ? x * 27500, calculated from sequence, x * 31000, SDS-PAGE Pseudomonas putida
1.1.1.227 ? x * 27500, calculated from sequence, x * 31000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.1.1.198 BDH
-
Pseudomonas sp. ATCC 17453
1.1.1.198 BDH1 cf. EC 1.1.1.227 Pseudomonas putida
1.1.1.227 BDH cf. EC 1.1.1.198 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.198 30
-
-
Pseudomonas putida
1.1.1.198 30
-
-
Pseudomonas sp. ATCC 17453
1.1.1.227 30
-
-
Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.198 0.006
-
NAD+ at pH 7.5 and 30°C Pseudomonas sp. ATCC 17453
1.1.1.198 0.07
-
(-)-borneol mutant enzyme S146A/Y188A, at pH 7.5 and 30°C Pseudomonas sp. ATCC 17453
1.1.1.198 0.13
-
(-)-borneol mutant enzyme S146A/Y188T, at pH 7.5 and 30°C Pseudomonas sp. ATCC 17453
1.1.1.198 0.89
-
(+)-borneol wild-type, pH 7.5, 30°C Pseudomonas putida
1.1.1.198 0.89
-
(+)-borneol at pH 7.5 and 30°C Pseudomonas sp. ATCC 17453
1.1.1.198 0.97
-
(-)-borneol at pH 7.5 and 30°C Pseudomonas sp. ATCC 17453
1.1.1.198 1.51
-
(+)-borneol mutant enzyme S146A/Y188A, at pH 7.5 and 30°C Pseudomonas sp. ATCC 17453
1.1.1.198 1.51
-
(+)-borneol mutant Y188A, pH 7.5, 30°C Pseudomonas putida
1.1.1.198 3.32
-
(+)-borneol mutant enzyme S146A/Y188T, at pH 7.5 and 30°C Pseudomonas sp. ATCC 17453
1.1.1.198 3.32
-
(+)-borneol mutant Y188T, pH 7.5, 30°C Pseudomonas putida
1.1.1.227 0.07
-
(-)-borneol mutant Y188A, pH 7.5, 30°C Pseudomonas putida
1.1.1.227 0.13
-
(-)-borneol mutant Y188T, pH 7.5, 30°C Pseudomonas putida
1.1.1.227 0.97
-
(-)-borneol wild-type, pH 7.5, 30°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.198 4.5
-
pH optimum for the reduction of camphor Pseudomonas sp. ATCC 17453
1.1.1.198 4.5
-
reduction of camphor Pseudomonas putida
1.1.1.198 7.5
-
oxidation of borneol Pseudomonas putida
1.1.1.198 7.5
-
pH optimum for the oxidation of (+/-)-borneol Pseudomonas sp. ATCC 17453
1.1.1.227 4.5
-
reduction of camphor Pseudomonas putida
1.1.1.227 7.5
-
oxidation of borneol Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.198 NAD+
-
Pseudomonas sp. ATCC 17453

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.198 Pseudomonas putida calculated from sequence
-
5.4
1.1.1.227 Pseudomonas putida calculated from sequence
-
5.4