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Literature summary extracted from

  • Park, J.; Lee, D.; Seo, E.K.; Ryu, J.S.; Shin, D.H.
    General assay for enzymes in the heptose biosynthesis pathways using electrospray ionization mass spectrometry (2017), Appl. Microbiol. Biotechnol., 101, 4521-4532 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.71 gene hddC or wcbM, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Burkholderia pseudomallei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.71 (-)-nyasol
-
 Burkholderia pseudomallei
2.7.7.71 additional information inhibitor screening Burkholderia pseudomallei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.71 additional information
-
 additional information steady-state kinetics Burkholderia pseudomallei
2.7.7.71 0.16
-
 GTP recombinant enzyme, pH 7.5, 37°C Burkholderia pseudomallei
2.7.7.71 0.33
-
 D-glucose 1-phosphate recombinant enzyme, pH 7.5, 37°C Burkholderia pseudomallei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.71 Mg2+ required Burkholderia pseudomallei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.71 D-glycero-alpha-D-manno-heptose 1-phosphate + GTP Burkholderia pseudomallei
-
 GDP-D-glycero-alpha-D-manno-heptose + diphosphate
-
 ?
2.7.7.71 D-glycero-alpha-D-manno-heptose 1-phosphate + GTP Burkholderia pseudomallei K96243
-
 GDP-D-glycero-alpha-D-manno-heptose + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.71 Burkholderia pseudomallei Q63R79
-
-
2.7.7.71 Burkholderia pseudomallei K96243 Q63R79
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.71 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography Burkholderia pseudomallei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.71 D-glucose 1-phosphate + GTP
-
 Burkholderia pseudomallei GDP-D-glucose + diphosphate
-
 ?
2.7.7.71 D-glucose 1-phosphate + GTP
-
 Burkholderia pseudomallei K96243 GDP-D-glucose + diphosphate
-
 ?
2.7.7.71 D-glycero-alpha-D-manno-heptose 1-phosphate + GTP
-
 Burkholderia pseudomallei GDP-D-glycero-alpha-D-manno-heptose + diphosphate
-
 ?
2.7.7.71 D-glycero-alpha-D-manno-heptose 1-phosphate + GTP
-
 Burkholderia pseudomallei K96243 GDP-D-glycero-alpha-D-manno-heptose + diphosphate
-
 ?
2.7.7.71 additional information the assay is performed with glucose 1-phosphate and GTP Burkholderia pseudomallei ?
-
-
2.7.7.71 additional information the assay is performed with glucose 1-phosphate and GTP Burkholderia pseudomallei K96243 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.7.71 D-glycero-alpha-D-manno-heptose-1-phosphate guanylyltransferase
-
 Burkholderia pseudomallei
2.7.7.71 HddC
-
 Burkholderia pseudomallei
2.7.7.71 WcbM
-
 Burkholderia pseudomallei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.71 37
-
 assay at Burkholderia pseudomallei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.71 0.0107
-
 GTP recombinant enzyme, pH 7.5, 37°C Burkholderia pseudomallei
2.7.7.71 0.0117
-
 D-glucose 1-phosphate recombinant enzyme, pH 7.5, 37°C Burkholderia pseudomallei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.71 7.5
-
 assay at Burkholderia pseudomallei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.71 0.0176
-
 recombinant enzyme, pH 7.5, 37°C, versus 1-phosphate and GTP Burkholderia pseudomallei (-)-nyasol

General Information

EC Number General Information Comment Organism
2.7.7.71 metabolism the GDP-D-alpha-D-heptose biosynthesis pathway (GDP-6dD-heptose pathway) includes D-glycero-alpha-D-manno-heptose-7-phosphate kinase (HddA or WcbL) and D-glycero-alpha-D-manno-heptose-1-phosphate guanylyltransferase (HddC or WcbM) instead of D-glycero-beta-D-manno-heptose-7-phosphate kinase (HldA) and D-glycero-beta-D-manno-heptose-1-phosphate adenylyltransferase (HldC), respectively. Identification of the activity of each enzyme in the GDP-6dD-heptose biosynthesis pathway by MS analysis, overview Burkholderia pseudomallei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.71 0.036
-
 D-glucose 1-phosphate recombinant enzyme, pH 7.5, 37°C Burkholderia pseudomallei
2.7.7.71 0.067
-
 GTP recombinant enzyme, pH 7.5, 37°C Burkholderia pseudomallei