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Literature summary extracted from

  • Motomura, K.; Hirota, R.; Okada, M.; Ikeda, T.; Ishida, T.; Kuroda, A.
    A new subfamily of polyphosphate kinase 2 (class III PPK2) catalyzes both nucleoside monophosphate phosphorylation and nucleoside diphosphate phosphorylation (2014), Appl. Environ. Microbiol., 80, 2602-2608 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.1 E126G about 18% of wild-type activity with substrate AMP, about 40% of wild-type activity with ADP Meiothermus ruber
2.7.4.1 E126N about 5% of wild-type activity with substrate AMP, bout 15% of wild-type activity with ADP Meiothermus ruber

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Mg2+ Mn2+ is preferred over Mg2+ Meiothermus ruber
2.7.4.1 Mn2+ Mn2+ is preferred over Mg2+ Meiothermus ruber
2.7.4.34 Mg2+ Mn2+ is preferred over Mg2+ Meiothermus ruber
2.7.4.34 Mn2+ Mn2+ is preferred over Mg2+ Meiothermus ruber

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Meiothermus ruber M9XB82 cf. EC 2.7.4.1
-
2.7.4.1 Meiothermus ruber NBRC 106122 M9XB82 cf. EC 2.7.4.1
-
2.7.4.34 Meiothermus ruber M9XB82 cf. EC 2.7.4.1
-
2.7.4.34 Meiothermus ruber NBRC 106122 M9XB82 cf. EC 2.7.4.1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.1 1.8
-
 substrate ADP, pH 7, 70°C Meiothermus ruber
2.7.4.1 99.1
-
 substrate AMP, pH 7, 70°C Meiothermus ruber

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ADP + (phosphate)n+1 enzyme prefers polyphosphate of 25 to 50 in chain length Meiothermus ruber ATP + (phosphate)n
-
 r
2.7.4.1 ADP + (phosphate)n+1 enzyme prefers polyphosphate of 25 to 50 in chain length Meiothermus ruber NBRC 106122 ATP + (phosphate)n
-
 r
2.7.4.1 AMP + (phosphate)n+2
-
 Meiothermus ruber ATP + (phosphate)n
-
 r
2.7.4.1 AMP + (phosphate)n+2
-
 Meiothermus ruber NBRC 106122 ATP + (phosphate)n
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Meiothermus ruber ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Meiothermus ruber NBRC 106122 ADP + (phosphate)n+1
-
 r
2.7.4.1 additional information enzyme is able to use nucleoside diphosphates ADP, GDP, CDP, and UDP Meiothermus ruber ?
-
-
2.7.4.1 additional information enzyme is able to use nucleoside diphosphates ADP, GDP, CDP, and UDP Meiothermus ruber NBRC 106122 ?
-
-
2.7.4.34 GDP + (phosphate)n+1 enzyme prefers polyphosphates of 25 to 50 in chain length Meiothermus ruber GTP + (phosphate)n
-
 r
2.7.4.34 GMP + (phosphate)n+2
-
 Meiothermus ruber GTP + (phosphate)n
-
 r
2.7.4.34 GTP + (phosphate)n
-
 Meiothermus ruber GDP + (phosphate)n+1
-
 r
2.7.4.34 additional information enzyme is able to use nucleoside diphosphates ADP, GDP, CDP, and UDP Meiothermus ruber ?
-
-
2.7.4.34 additional information enzyme is able to use nucleoside diphosphates ADP, GDP, CDP, and UDP Meiothermus ruber NBRC 106122 ?
-
-

Subunits

EC Number Subunits Comment Organism
2.7.4.1 ? x * 32000, SDS-PAGE, x * 31600, calculated from sequence Meiothermus ruber
2.7.4.34 ? x * 32000, SDS-PAGE, x * 31600, calculated from sequence Meiothermus ruber

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 K649_10090
-
 Meiothermus ruber
2.7.4.1 Mrub_2488
-
 Meiothermus ruber
2.7.4.34 K649_10090
-
 Meiothermus ruber
2.7.4.34 Mrub_2488
-
 Meiothermus ruber

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.1 60 70
-
 Meiothermus ruber
2.7.4.34 60 70
-
 Meiothermus ruber

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.1 70
-
 stable in the presence of both Mn2+ and polyphosphate but immediately loses its activity in the absence of either n2+ or polyphosphate Meiothermus ruber
2.7.4.34 70
-
 stable in the presence of both Mn2+ and polyphosphate but immediately loses its activity in the absence of either Mn2+ or polyphosphate Meiothermus ruber