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Literature summary extracted from

  • Wang, D.M.; Sun, L.; Sun, W.J.; Cui, F.J.; Gong, J.S.; Zhang, X.M.; Shi, J.S.; Xu, Z.H.
    A membrane-bound gluconate dehydrogenase from 2-keto-D-gluconic acid industrial producing strain Pseudomonas plecoglossicida JUIM01 Purification, characterization, and gene identification (2019), Appl. Biochem. Biotechnol., 188, 897-913 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.215 expression in Escherichia coli Pseudomonas plecoglossicida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.215 additional information not inhibitory: EDTA Pseudomonas plecoglossicida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.215 0.63
-
 D-gluconate pH 6, 25°C Pseudomonas plecoglossicida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.215 membrane
-
 Pseudomonas plecoglossicida 16020
-

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.215 Acetone 40% v/v, 32% residual activity Pseudomonas plecoglossicida
1.1.1.215 Ethanol 40% v/v, complete loss of activity Pseudomonas plecoglossicida
1.1.1.215 hexane 40% v/v, 39% residual activity Pseudomonas plecoglossicida
1.1.1.215 isopentanol 40% v/v, complete loss of activity Pseudomonas plecoglossicida
1.1.1.215 Methanol 40% v/v, complete loss of activity Pseudomonas plecoglossicida

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.215 Pseudomonas plecoglossicida A0A2H4PJM1 and A0A2H4PJM2 and A0A2H4PJM7 A0A2H4PJM1 i.e gamma subunit, A0A2H4PJM2 i.e. FAD containing subunit, A0A2H4PJM7 i.e. cytochrome c subunit
-
1.1.1.215 Pseudomonas plecoglossicida JUIM01 A0A2H4PJM1 and A0A2H4PJM2 and A0A2H4PJM7 A0A2H4PJM1 i.e gamma subunit, A0A2H4PJM2 i.e. FAD containing subunit, A0A2H4PJM7 i.e. cytochrome c subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.215 native enzyme, via ultrasonification and ultracentrifugation, phase separation, ammonium sulfate precipitation, and DEAE-Sepharose and hydroxyapatite chromatography Pseudomonas plecoglossicida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.215 90.71
-
 pH 6, 35°C Pseudomonas plecoglossicida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.215 D-gluconate + NADP+
-
 Pseudomonas plecoglossicida 2-dehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.215 D-gluconate + NADP+
-
 Pseudomonas plecoglossicida JUIM01 2-dehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.215 additional information no activity on other sugars and sugar acids Pseudomonas plecoglossicida ?
-
-
1.1.1.215 additional information no activity on other sugars and sugar acids Pseudomonas plecoglossicida JUIM01 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.1.1.215 More enzyme consists of a gamma subunit, a flavoprotein subunit, and a cytochrome c subunit with molecular masses of about 27, 65, and 47 kDa, respectively Pseudomonas plecoglossicida

Synonyms

EC Number Synonyms Comment Organism
1.1.1.215 GADH
-
 Pseudomonas plecoglossicida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.215 35
-
-
 Pseudomonas plecoglossicida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.215 6
-
-
 Pseudomonas plecoglossicida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.215 8
-
 no activity above Pseudomonas plecoglossicida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.215 FAD
-
 Pseudomonas plecoglossicida